Immobilization of Beauveria bassiana Lipase on Silica Gel by Physical Adsorption

Sugahara, Vanessa Hitomi; Varéa, Geni da Silva

doi:10.1590/s1516-8913201401358

Cited by 16 publications

(7 citation statements)

References 26 publications

(33 reference statements)

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“…Microbial lipases are the preferred potent source due to several industrial potentials (Hasan et al 2006;Riaz et al 2010;Veerapagu et al 2013). They are present in many bacteria, fungi, plants, and animals (Rabbani et al 2013;Sugahara et al 2014). A considerable number of lipase producing microorganisms such as Pseudomonas fragi, Staphylococcus aureus, and Burkholderia glumae have been described (Ghanem et al 2000;Gupta et al 2004;Sangeetha et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Iqbal

Rehman

2015

Braz. arch. biol. technol.

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Section: Introductionmentioning

confidence: 99%

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Iqbal

Rehman

2015

Braz. arch. biol. technol.

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“…The adsorption of pectinase on the epoxy carrier activated by polyethyleneimine was carried out with an efficiency of 12% calculated based on the activity of the enzyme [ 30 ]. The efficiency of immobilization by the adsorption method at the level of a few to several percent is a typical value of this method of immobilization [ 52 , 53 , 54 , 55 , 56 , 57 ]. Significantly higher enzyme immobilization efficiencies, including naringinase, are obtained when using other methods, e.g., gel entrapment [ 28 , 47 , 48 , 54 ].…”

Section: Resultsmentioning

confidence: 99%

Immobilization of Naringinase from Aspergillus Niger on a Magnetic Polysaccharide Carrier

Bodakowska-Boczniewicz

Garncarek

2020

Molecules

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Naringinase is an enzymatic complex used in the deglycosylation of compounds with a high application potential in the food and pharmaceutical industries. The aim of the study was to immobilize naringinase from Aspergillus niger KMS on a magnetic carrier obtained on the basis of carob gum activated by polyethyleneimine. Response surface methodology was used to optimize naringinase immobilization taking into account the following factors: pH, immobilization time, initial concentration of naringinase and immobilization temperature. The adsorption of the enzyme on a magnetic carrier was a reversible process. The binding force of naringinase was increased by crosslinking the enzyme with the carrier using dextran aldehyde. The crosslinked enzyme had better stability in an acidic environment and at a higher temperature compared to the free form. The immobilization and stabilization of naringinase by dextran aldehyde on the magnetic polysaccharide carrier lowered the activation energy, thus increasing the catalytic capacity of the investigated enzyme and increasing the activation energy of the thermal deactivation process, which confirms higher stability of the immobilized enzyme in comparison with free naringinase. The preparation of crosslinked naringinase retained over 80% of its initial activity after 10 runs of naringin hydrolysis from fresh and model grapefruit juice.

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“…Physical adsorption method is one of the simplest immobilization methods; in this method, the enzyme is adsorbed on the surface of the matrix that may be an organic or inorganic matrix (Woodward 1985;Sugahara and Varéa 2014). This adsorption method is based on weak bonds such as van der Waal's force, electrostatic and hydrophobic interactions, hydrogen bond or ionic bond between enzyme and solid matrix (Flickinger and Drew 1999;Jegannathan et al 2008).…”

Section: Adsorptionmentioning

confidence: 99%

Impact of immobilization technology in industrial and pharmaceutical applications

et al. 2019

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The current demands of the world's biotechnological industries are enhancement in enzyme productivity and development of novel techniques for increasing their shelf life. Compared to free enzymes in solution, immobilized enzymes are more robust and more resistant to environmental changes. More importantly, the heterogeneity of the immobilized enzyme systems allows an easy recovery of both enzymes and products, multiple reuse of enzymes, continuous operation of enzymatic processes, rapid termination of reactions, and greater variety of bioreactor designs. This review summarizes immobilization definition, different immobilization methods, advantages and disadvantages of each method. In addition, it covers some food industries, protein purification, human nutrition, biodiesel production, and textile industry. In these industries, the use of enzymes has become an inevitable processing strategy when a perfect end product is desired. It also can be used in many other important industries including health care and pharmaceuticals applications. One of the best uses of enzymes in the modern life is their application in diagnose and treatment of many disease especially when used in drug delivery system or when used in nanoform.

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Immobilization of Beauveria bassiana Lipase on Silica Gel by Physical Adsorption

Abstract: ABSTRACT

Cited by 16 publications

References 26 publications

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Immobilization of Naringinase from Aspergillus Niger on a Magnetic Polysaccharide Carrier

Impact of immobilization technology in industrial and pharmaceutical applications

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