The entomopathogenic fungus Beauveria bassiana CG432 produced an extracellular lipase on the 7 th day of fermentation in Alves broth containing 0.25% Triton X-100 and Tween 80 surfactants, and 3.0% olive oil at 28°C and 200rpm. Optimal temperature and pH conditions for lipase activity were found to be range from 40 to 60°C and pH 7.5 and 8.0 using 50 mM Tris-HCl buffer, verified by a hydrolysis p-nitrophenol palmitate assay. Under these conditions, lipase was 50% stable for approximately 10h, however it was stable for up to 10 days (-18°C), refrigerated (4°C) or kept at room temperature (25°C).
Keywords
RESUMOO fungo entomopatogênico Beauveria bassiana CG432 produziu uma lipase extracelular no sétimo dia de cultivo em meio líquido de Alves contendo 0,25% dos surfactantes Triton X-100 e Tween 80 e 3,0% de óleo de oliva e 200 rpm. A temperatura e o pH ótimos de atividade na faixa de temperatura na faixa de 40 a 60ºC, e pH entre 7,5 e 8,0 em tampão TRIS-HCl 5mM utilizando a reação de hidrólise do substrato palmitato de p-nitrofenol. Sob tais condições, a lipase permaneceu ativa em 50% de sua atividade total durante 10 horas, e foi estável por mais de 10 dias quando armazenada sob congelamento (-18C, refrigeração (4ºC) e a 25C.Palavras-chave: Beauveria bassiana; produção de lipase, óleos vegetais, resíduos oleosos, desodorização
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