In vitro evaluation of adhesion and aggregation abilities of four potential probiotic strains isolated from guppy (Poecilia reticulata)

Balakrishna, Aparna

doi:10.1590/s1516-89132013000500010

Cited by 36 publications

(28 citation statements)

References 34 publications

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“…The obtained results were in agreement with those reported by Blakrishma (36); as he showed that there is no correlation between these parameters. In another study, Lp9 isolate has surface hydrophobicity of 37-38%, suggesting its adhesiveness (37).…”

Section: Discussionsupporting

confidence: 92%

Technological and Probiotic Traits of the Lactobacilli Isolated From Vaginal Tract of the Healthy Women for Probiotic Use

Bouridane

Sifour

Idoui

et al. 2016

Iran. J. Biotechnol.

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Background For biotechnological application, selected lactic acid bacteria strains belonging to the genera Lactobacillus (Lb) are proposed as an alternative to the antibiotics for the prevention and treatment of urogenital tract infections. Objectives Isolating and selecting vaginal lactobacilli strains for probiotic use based on their technological and probiotic aptitudes. Materials and Methods The vaginal isolates were examined for their essential characteristics as the potential probiotic such as low pH tolerance, bile-salt and simulated human intestinal fluid (SIF) resistance, adhesion to the vaginal epithelial cells (VECs), aggregation and coaggregation, surface hydrophobicity, antimicrobial activity, acid production, antibiotic resistance, and resistance to spermicides. The best strain was identified by PCR. Results From 70 lactobacilli isolates and according to the 16 rDNA sequences, isolates B6 and B10 showed the closest homology (99%) to the Lb. gasseri and Lb. plantarum respectively. They produced hydrogen peroxide (H2O2), tolerant to acid, bile, simulated human intestinal fluid, present a strong adhesion, highest percentages of aggregation, and antibacterial activity. These strains are resistant to the spermicide and actively acidify the growth medium. Conclusions Strains Lb. plantarum B10 and Lb. gasseri B6 have a strong potential probiotic confirming their value as a tool for prevention against urinary and vaginal infections.

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Section: Discussionsupporting

confidence: 92%

Technological and Probiotic Traits of the Lactobacilli Isolated From Vaginal Tract of the Healthy Women for Probiotic Use

Bouridane

Sifour

Idoui

et al. 2016

Iran. J. Biotechnol.

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“…Furthermore, the coaggregation and AAg were found to be directly related properties. The results are in agreement with earlier reports [6,36]. Nature of cell surface components of probiotics/pathogens may account for differences in coaggregation and AAg [1,2].…”

Section: Discussionsupporting

confidence: 93%

“…Coaggregation ability of probiotics with pathogens is considered as a desired property as it may play an important role in eliminating the pathogens from the GIT by preventing their attachment/lodging to the host tissue [35]. Varying level of coaggregation ability of the LAB isolates with different pathogens indicated that coaggregation potential is specific attribute that depends upon type of probiotic strain and the specific pathogens [36]. Furthermore, the coaggregation and AAg were found to be directly related properties.…”

Section: Discussionmentioning

confidence: 99%

Phytase-Producing Potential and Other Functional Attributes of Lactic Acid Bacteria Isolates for Prospective Probiotic Applications

Andrabi

Bhat

Gupta

et al. 2016

Probiotics & Antimicro. Prot.

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Wide variations among multifaceted-health benefitting attributes of probiotics fueled investigations on targeting efficacious probiotics. In the current study, lactic acid bacteria (LAB) isolated from poultry gut, feces of rat, chicken, human infants, and fermented foods were characterized for desired probiotic functional properties including the phytase-producing ability which is one of the wanted characteristics for probiotics for potential applications for upgrading animal nutrition, enhancing feed conversion, and minimizing anti-nutritional properties. Among 62 LAB isolates Weissella kimchii R-3 an isolate from poultry gut exhibited substantial phytase-producing ability (1.77 U/ml) in addition to other functional probiotic characteristics viz. hydrophobicity, autoaggregation, coaggregation with bacterial pathogens, and antimicrobial activity against pathogens. Survival of W. kimchii R-3 cells (in free and calcium alginate encapsulated state) was examined sequentially in simulated gastric and intestinal juices. Encapsulated cells exhibited better survival under simulated gut conditions indicating that encapsulation conferred considerable protection against adverse gut conditions. Furthermore, simulated gastric and intestinal juices with pepsin and pancreatin showed higher survival of cells than the juices without pepsin and pancreatin. W. kimchii R-3 due to its significant functional probiotic attributes may have prospective for commercial applications in human/animal nutrition.

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“…The results revealed that aggregation, hydrophobicity and adhesion of LAB strains are strain‐specific and the correlations between autoaggregation and hydrophobicity, and autoaggregation and adhesion are positive among the nine L. lactis strains rather than the 18 strains belonging to different species tested. Therefore, bacterial aggregation and hydrophobicity can be used as a preliminary screening to assess their adhesion properties (Balakrishna ). In addition, porcine ileal epithelial cells were used in in vitro models to investigate aggregation and adhesiveness of Lactobacillus acidophilus M92 (Kos et al .…”

Section: In Vitro and Ex Vivo Studiesmentioning

confidence: 99%

The adherence and colonization of microorganisms in fish gastrointestinal tract

Ringø

Hoseinifar

et al. 2018

Reviews in Aquaculture

104

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Eggs, gills, skin and intestinal tracts of fish harbour bacterial communities that are thought to impact fish health through their interaction with the tissues. In particular, the gastrointestinal (GI) tract possesses numerous microbes and is considered an infection route. During the infections caused by pathogenic bacteria, mucosal adhesion is a critical early phase and plays an important role in fish development. This review focused on knowledge regarding the adherence and colonization of microorganisms in fish GI tract mucus, including various methods such as in vivo, in vitro, and ex vivo, host specificity, lactic acid bacteria nonfish origin using the adherence of pathogenic bacteria, gnotobiotic approaches and molecular methods. However, probiotics might not be possible to truly colonize the digestive tract of fish. The term 'temporal colonization' might be a better phrase to use when discussing probiotic persistence and presence within the mucosal layer. Recent and promising results in this field were presented based on new perspectives and future research directions of the adherence and colonization of microorganisms in fish GI tracts.

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In vitro evaluation of adhesion and aggregation abilities of four potential probiotic strains isolated from guppy (Poecilia reticulata)

Cited by 36 publications

References 34 publications

Technological and Probiotic Traits of the Lactobacilli Isolated From Vaginal Tract of the Healthy Women for Probiotic Use

Technological and Probiotic Traits of the Lactobacilli Isolated From Vaginal Tract of the Healthy Women for Probiotic Use

Phytase-Producing Potential and Other Functional Attributes of Lactic Acid Bacteria Isolates for Prospective Probiotic Applications

The adherence and colonization of microorganisms in fish gastrointestinal tract

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