Comparison of two lipid extraction methods produced by yeast in cheese whey

Castanha, Rodrigo Fernandes; Morais, L. A. S. de; Mariano, Adriano Pinto; Monteiro, Regina Teresa Rosim

doi:10.1590/s1516-89132013000400013

Cited by 19 publications

(8 citation statements)

References 40 publications

(42 reference statements)

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“…The use of DCW as a substrate for SCO production was properly investigated and some encouraging results have been obtained. For instance, Taskin et al (2015) reported lipid content of 58% (wt/wt) during growth of Yarrowia lipolytica on lactose enriched deproteinized whey-based medium in batch shake flask cultures, while Castanha et al (2013) obtained SCO production of 13.1% (wt/wt) when Cryptococcus laurentii was cultivated on DCW.…”

Section: Resultsmentioning

confidence: 99%

A sustainable use of low-cost raw substrates for biodiesel production by the oleaginous yeast Wickerhamomyces anomalus

2017

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Over the past decade, the increasing demand of vegetable oils for biodiesel production has highlighted the need for alternative oil feedstocks that do not compete with food production. In this context, the combined use of agro-industrial wastes and oleaginous microorganisms could be a promising strategy for sustainable biodiesel production. The present investigation involves the performance of the oleaginous yeast strain EC28 to produce lipids from different agro-industrial wastewaters (i.e., deproteinized cheese whey, olive mill wastewater, and wastewaters from confectionary industries) and waste frying oils (i.e., waste oil from frying fish, waste oil from frying potato and waste oil from frying meat). Results indicated that this strain can adequately grow on agro-industrial wastewater-based media and produce substantial amounts of lipids [up to 24%, wt/wt in deproteinized cheese whey-based medium and olive mill wastewater-based medium (75%, v/v in water)] of similar fatty acid composition to that of the most commonly used vegetable oils in the biodiesel industry. However, the addition of frying oils to the culture media resulted in a significant decrease in total lipid content, probably due to excess of available nitrogen released from meat, fish, and potato into the frying oil. The estimated properties of the resulting biodiesels, such as SV (190.69-203.13), IV (61.77-88.32), CN (53.45-59.32), and CFPP (-0.54 to 10.4), are reported, for the first time, for and correlate well with specified standards. In conclusion, strain EC28, for which there is very limited amount of available information, might be regarded as a promising candidate for biodiesel production and additional efforts for process improvement should be envisaged.

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Section: Resultsmentioning

confidence: 99%

A sustainable use of low-cost raw substrates for biodiesel production by the oleaginous yeast Wickerhamomyces anomalus

2017

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“…Additionally, the Bligh and Dyer method has a significantly higher lipid yield than the Folch extraction method (1.44‐fold larger). Higher lipid yields using the Bligh and Dyer extraction method compared to the Folch method were also observed in microalgae and yeast (Ryckebosch et al ; Castanha et al ). Iverson et al () found that Folch achieved superior lipid extraction compared to Bligh and Dyer in a variety of marine animal tissues that were greater than two percent lipid by weight (Iverson et al ).…”

Section: Discussionmentioning

confidence: 92%

Methodological biases in estimates of macroalgal macromolecular composition

Fiset

Liefer

Irwin

et al. 2017

Limnology & Ocean Methods

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Interest surrounding the use of macroalgae macromolecules for food products, biofuels, or other industrial applications is growing. As researchers search for macroalgae with especially high protein, lipid, carbohydrate or fibre content, the demand for a suite of standardized and unbiased methods for quantifying macroalgae macromolecules increases. Using data from available scientific literature, we evaluated the biases of the major methods used to determine macroalgal macromolecular content, as well as the sample drying methods employed. We found that drying at room temperature prior to analysis resulted in the highest estimates of protein and carbohydrate, and that freeze‐drying provided the highest estimates of lipid. Using nitrogen content and the standard conversion factor to calculate protein in macroalgae (N × 6.25 method) overestimates protein content compared to protein assays such as the Bradford () or Lowry () assays. The Bligh and Dyer () lipid extraction method was found to have a yield nearly two‐fold higher than other standard methods. For carbohydrates, the By Difference and Prosky et al. () methods provide estimates up to five‐fold higher than other common methods used to determine carbohydrate and fibre. Based on these results we recommend using protein assays as opposed to nitrogen content assays to determine protein content, the Bligh and Dyer lipid extraction method for lipids, and the By Difference and Prosky method for carbohydrate and fibre, respectively.

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“…We vary the biomass titer in the range of 0.5–200 g/L as it includes the range for intracellular insoluble products in photoautotrophic (0.5–10 g/L) [ 118 , 120 ] as well as heterotrophic (10–200 g/L) [ 53 , 119 ] conditions.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and analysis of separation networks for the recovery of intracellular chemicals generated from microbial-based conversions

Yenkie

Maravelias

2017

Biotechnol Biofuels

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BackgroundBioseparations can contribute to more than 70% in the total production cost of a bio-based chemical, and if the desired chemical is localized intracellularly, there can be additional challenges associated with its recovery. Based on the properties of the desired chemical and other components in the stream, there can be multiple feasible options for product recovery. These options are composed of several alternative technologies, performing similar tasks. The suitability of a technology for a particular chemical depends on (1) its performance parameters, such as separation efficiency; (2) cost or amount of added separating agent; (3) properties of the bioreactor effluent (e.g., biomass titer, product content); and (4) final product specifications. Our goal is to first synthesize alternative separation options and then analyze how technology selection affects the overall process economics. To achieve this, we propose an optimization-based framework that helps in identifying the critical technologies and parameters.ResultsWe study the separation networks for two representative classes of chemicals based on their properties. The separation network is divided into three stages: cell and product isolation (stage I), product concentration (II), and product purification and refining (III). Each stage exploits differences in specific product properties for achieving the desired product quality. The cost contribution analysis for the two cases (intracellular insoluble and intracellular soluble) reveals that stage I is the key cost contributor (>70% of the overall cost). Further analysis suggests that changes in input conditions and technology performance parameters lead to new designs primarily in stage I.ConclusionsThe proposed framework provides significant insights for technology selection and assists in making informed decisions regarding technologies that should be used in combination for a given set of stream/product properties and final output specifications. Additionally, the parametric sensitivity provides an opportunity to make crucial design and selection decisions in a comprehensive and rational manner. This will prove valuable in the selection of chemicals to be produced using bioconversions (bioproducts) as well as in creating better bioseparation flow sheets for detailed economic assessment and process implementation on the commercial scale.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0804-2) contains supplementary material, which is available to authorized users.

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Comparison of two lipid extraction methods produced by yeast in cheese whey

Abstract: ABSTRACT

Cited by 19 publications

References 40 publications

A sustainable use of low-cost raw substrates for biodiesel production by the oleaginous yeast Wickerhamomyces anomalus

A sustainable use of low-cost raw substrates for biodiesel production by the oleaginous yeast Wickerhamomyces anomalus

Methodological biases in estimates of macroalgal macromolecular composition

Synthesis and analysis of separation networks for the recovery of intracellular chemicals generated from microbial-based conversions

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