Synthesis and analysis of separation networks for the recovery of intracellular chemicals generated from microbial-based conversions

Yenkie, Kirti M.; Wu, Wenzhao; Maravelias, Christos T.

doi:10.1186/s13068-017-0804-2

Cited by 18 publications

(32 citation statements)

References 126 publications

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“…The feasibility of these intracellular metabolite production processes eventually depends on the efficiency of the down-streaming for complete product recovery. Existing methodologies for intracellular oil recovery are based on energy-intense cell-disruptive techniques which account for about 70% of total production costs [7]. While, others suggest direct hydro-thermal conversions of the oleaginous cells into liquefied high energy drop-in fuel precursors [8].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica

Pawar

Odaneth

Vadgama

et al. 2019

Biotechnol Biofuels

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Background Recent trends in bioprocessing have underlined the significance of lignocellulosic biomass conversions for biofuel production. These conversions demand at least 90% energy upgradation of cellulosic sugars to generate renewable drop-in biofuel precursors (Heff/C ~ 2). Chemical methods fail to achieve this without substantial loss of carbon; whereas, oleaginous biological systems propose a greener upgradation route by producing oil from sugars with 30% theoretical yields. However, these oleaginous systems cannot compete with the commercial volumes of vegetable oils in terms of overall oil yields and productivities. One of the significant challenges in the commercial exploitation of these microbial oils lies in the inefficient recovery of the produced oil. This issue has been addressed using highly selective oil capturing agents (OCA), which allow a concomitant microbial oil production and in situ oil recovery process. Results Adsorbent-based oil capturing agents were employed for simultaneous in situ oil recovery in the fermentative production broths. Yarrowia lipolytica, a model oleaginous yeast, was milked incessantly for oil production over 380 h in a media comprising of glucose as a sole carbon and nutrient source. This was achieved by continuous online capture of extracellular oil from the aqueous media and also the cell surface, by fluidizing the fermentation broth over an adsorbent bed of oil capturing agents (OCA). A consistent oil yield of 0.33 g per g of glucose consumed, corresponding to theoretical oil yield over glucose, was achieved using this approach. While the incorporation of the OCA increased the oil content up to 89% with complete substrate consumptions, it also caused an overall process integration. Conclusion The nondisruptive oil capture mediated by an OCA helped in accomplishing a trade-off between microbial oil production and its recovery. This strategy helped in realizing theoretically efficient sugar-to-oil bioconversions in a continuous production process. The process, therefore, endorses a sustainable production of molecular drop-in equivalents through oleaginous yeasts, representing as an absolute microbial oil factory.

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Section: Introductionmentioning

confidence: 99%

Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica

Pawar

Odaneth

Vadgama

et al. 2019

Biotechnol Biofuels

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“…We will use the abbreviations when referring to the specific technologies hereafter. For discussions regarding the three stage scheme and the specific separation technologies, the readers are referred to [42,50,51]. We would like to point out that we have chosen the most basic configurations for the technology models.…”

Section: Stage-wise Separation Schemementioning

confidence: 99%

“…The active streams are shown by bold red lines and selected technologies are highlighted in different colors corresponding to each stage: red for stage I, green for stage II, and blue for stage III. Cost distribution is shown by the numbers on the left bar the previous study on intracellular products [51], the major cost driver was Stage I irrespective of the other properties of the product, however, for extracellular products, Stage II costs take precedence and have a major impact on the overall downstream separation network selection.…”

Section: Alternative Concentration Options In Stage IImentioning

confidence: 99%

“…For a volatile (VOL) product, the product will be obtained at the top instead of at the bottom in Dst. Also, direct Dst is typically cheaper than Ext or Atpe when the relative volatility is greater than 1.05 [51,113]. For a specialty (SPC) product, the same argument about additional purification and stringent requirements in Stage III technologies will be valid due to high purity requirements.…”

Section: Extension To Other Classes Of Ex Sol Productsmentioning

confidence: 99%

“…However, these studies were mostly focused on either general methodological discussions or analysis for specific products on a case-by-case basis. The recent studies by Maravelias group [42,50,51] have presented a systematic approach for generating and modeling bio-separation superstructures for different classes of products defined in terms of a set of attributes including product localization, solubility, density, volatility, physical state, and intended use. This work builds on those recent studies to look at microbial products released extracellularly in more detail through carefully selected case studies, appropriate parameter values, typical range of variation and additional insights derived from solving multiple optimization problems.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions

Yenkie

Maravelias

2019

BMC Chem Eng

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Recent advances in metabolic engineering have enabled the production of chemicals via bio-conversion using microbes. However, downstream separation accounts for 60-80% of the total production cost in many cases. Previous work on microbial production of extracellular chemicals has been mainly restricted to microbiology, biochemistry, metabolomics, or techno-economic analysis for specific product examples such as succinic acid, xanthan gum, lycopene, etc. In these studies, microbial production and separation technologies were selected apriori without considering any competing alternatives. However, technology selection in downstream separation and purification processes can have a major impact on the overall costs, product recovery, and purity. To this end, we apply a superstructure optimization based framework that enables the identification of critical technologies and their associated parameters in the synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions. We divide extracellular chemicals into three categories based on their physical properties, such as water solubility, physical state, relative density, volatility, etc. We analyze three major extracellular product categories (insoluble light, insoluble heavy and soluble) in detail and provide suggestions for additional product categories through extension of our analysis framework. The proposed analysis and results provide significant insights for technology selection and enable streamlined decision making when faced with any microbial product that is released extracellularly. The parameter variability analysis for the product as well as the associated technologies and comparison with novel alternatives is a key feature which forms the basis for designing better bioseparation strategies that have potential for commercial scalability and can compete with traditional chemical production methods.

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Evaluation of isoflavone extraction options at commercial scale

Chea

Lehr

Stanzione

et al. 2022

Biofuels Bioprod Bioref

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Resource sustainability concerns keep increasing owing to the continuous use of natural materials without reclamation. Efforts are ongoing to find sustainable feedstocks for naturally derived products with competitive economics and properties. However, there is no established methodology for commercializing the extraction of chemicals from renewable sources. This work examined soybeans as a potential bio‐based feedstock to generate isoflavones at a commercial scale. The isoflavone content in soybeans has proven beneficial in the nutraceuticals industry for its anti‐inflammatory and cancer‐inhibition properties and in the materials sector owing to their inherent chemical functionality and thermal resistance. Currently, isoflavone extraction is only optimized at lab scale. Thus, we present a superstructure‐based framework to screen isoflavone extraction methods and assess their viability at a commercial scale. This allows the simultaneous comparison of alternative technologies using mixed‐integer nonlinear programming optimization to determine the most economical pathway. Internal material recovery greatly enhances the viability at a commercial scale by reducing the annual operating cost and environmental footprint. Therefore, this work presents a powerful tool for systematically comparing extraction pathways and assessing their commercial feasibility. The successful implementation of commercial‐scale extraction of isoflavone has tremendous potential for generating additional revenue for US soy farmers. Isoflavones can produce bio‐based polymers and composites and are used in products such as dietary supplements, cosmetics and nutraceuticals. Similar works can be applied to extract other natural materials from renewable feedstocks and increase the production of bio‐based products. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.

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Synthesis and analysis of separation networks for the recovery of intracellular chemicals generated from microbial-based conversions

Cited by 18 publications

References 126 publications

Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica

Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica

Synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions

Evaluation of isoflavone extraction options at commercial scale

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