Cloning, expression, purification and assay of sorbitol dehydrogenase from "Feicheng" peach fruit (Prunus persica)

Sun, Zhen; Ma, Chi; Zhou, Jie; Zhu, Shuhua

doi:10.1590/s1516-89132013000400002

Cited by 3 publications

(1 citation statement)

References 25 publications

(28 reference statements)

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“…Among all the analytes present in the reaction mixture, only SDH gives the emission peak at 461 nm. But in the emission spectra of reaction mixture, in the absence of caffeine this peak is absent, because SDH is consumed up during the reaction [36]. Again, when the reaction mixture is treated with caffeine, the fluorescence emission peak intensity of NADH (at 434 nm) undergoes an appreciable change, that is, it decreases immediately as the formation of NADH is hindered.…”

Section: Resultsmentioning

confidence: 99%

Free serum sorbitol and its interaction with caffeine: A suggestive approach for plausible remediation of diabetic neuropathy

Srivastava

Sen

2020

Biotech and App Biochem

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The measure of sorbitol in serum can act as a good indicator in the monitoring of the diabetic complications. To analyze the sorbitol level in serum medium, fluorometric enzymatic assay was performed. To remove the excess sorbitol from the body, proposed binding of sorbitol with caffeine was investigated. Their interaction in serum medium was studied and established by UV-Vis, fluorescence spectrophotometry, and time-correlated single photon counting (TCSPC). The linear calibration of sorbitol (in the range 10-50 mM) was done using UV-Vis spectrophotometry. Time scan experiments furnished the reaction rate of sorbitol assayed solution as well as sorbitol-caffeine complex as 0.021 min −1 and 0.018 min −1 , respectively. A sudden drop was observed in the fluorescence lifetime of reduced nicotinamide adenine dinucleotide (NADH) present in sorbitol assayed solution upon complexation with caffeine, that is, from 1.774 × 10 −09 to 1.23 × 10 −10 Sec, which indicates the hindrance in the formation of NADH and the probable formation of some other species. Isothermal titration calorimetric experiments clearly indicate the number of binding sites (i.e., 3.89, 1.40, and 2.07) that exist between sorbitol and caffeine at the complexation ratio of 1:1.2, 1:1.5, and 1:3. The present method can be helpful in pharmacological and therapeutic studies of sorbitol using caffeine for treating diabetic neuropathy.

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Section: Resultsmentioning

confidence: 99%

Free serum sorbitol and its interaction with caffeine: A suggestive approach for plausible remediation of diabetic neuropathy

Srivastava

Sen

2020

Biotech and App Biochem

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Response of carbohydrate metabolism-mediated sink strength to auxin in shoot tips of apple plants

Zhu

et al. 2022

Journal of Integrative Agriculture

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Effects of Spraying Calcium Fertilizer on Photosynthesis, Mineral Content, Sugar–Acid Metabolism and Fruit Quality of Fuji Apples

Wang

Han

et al. 2022

Agronomy

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This study was conducted at the Taidong Base of the Shandong Institute of Pomology, Tai’an City, Shandong Province, China, from May to October 2020. The effects of spraying calcium fertilizer on the photosynthetic characteristics, mineral element content, sugar and acid metabolism, and quality of apples were assessed. Fuji Apple Tianhong.2/SH/Malus robusta (M. robusta) was treated with the calcium fertilizers Niucui (Ca ≥ 100 g/L, B: 1–10 g/L) and Naipu 9 (Ca: 90 g/L, Mg: 21 g/L, N: 110 g/L) at the young fruit stage (25 May), the expansion stage (5 July), and the coloring stage (25 September). Water was sprayed as the control application. The results revealed that spraying calcium fertilizer increased the chloroplast pigment content and photosynthetic capacity of the apple leaves. Compared with the control, the net photosynthetic rate of apple leaves sprayed with Niucui increased by 4.3–34.6%, and that of leaves sprayed with Naipu 9 increased by 15.0–57.4%. Spraying calcium fertilizer promoted the accumulation of Ca, Mg, and B mineral elements in leaves and fruits while inhibiting the accumulation of Cu. Spraying calcium fertilizer improved the quality of apple fruit. Compared to the control fruit, the single fruit weight, fruit hardness, soluble solid content, and Vitamin C content of fruit sprayed with Niucui and Naipu 9 increased by 6.5 and 12.1%, 3.6 and 16.1%, 6.3 and 12.0%, and 30.5 and 29.4%, respectively. Spraying calcium fertilizer increased the sugar content and decreased the acid content of the apples. Naipu 9 was more effective at increasing fruit sugar content, especially sucrose and sorbitol, while Niucui was more effective at reducing the acid content, especially malic acid. This was observed because Naipu 9 increased the sugar metabolism enzyme activity, while Niucui increased the acid decomposition-related enzyme activity. In conclusion, according to the nutrition quality and flavor of the fruit, Naipu 9 application was determined to be the best fertilizer for the Fuji apple.

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Cloning, expression, purification and assay of sorbitol dehydrogenase from "Feicheng" peach fruit (Prunus persica)

Abstract: ABSTRACT

Cited by 3 publications

References 25 publications

Free serum sorbitol and its interaction with caffeine: A suggestive approach for plausible remediation of diabetic neuropathy

Free serum sorbitol and its interaction with caffeine: A suggestive approach for plausible remediation of diabetic neuropathy

Response of carbohydrate metabolism-mediated sink strength to auxin in shoot tips of apple plants

Effects of Spraying Calcium Fertilizer on Photosynthesis, Mineral Content, Sugar–Acid Metabolism and Fruit Quality of Fuji Apples

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