Free serum sorbitol and its interaction with caffeine: A suggestive approach for plausible remediation of diabetic neuropathy

Abstract

The measure of sorbitol in serum can act as a good indicator in the monitoring of the diabetic complications. To analyze the sorbitol level in serum medium, fluorometric enzymatic assay was performed. To remove the excess sorbitol from the body, proposed binding of sorbitol with caffeine was investigated. Their interaction in serum medium was studied and established by UV-Vis, fluorescence spectrophotometry, and time-correlated single photon counting (TCSPC). The linear calibration of sorbitol (in the range 10-50 mM) was done using UV-Vis spectrophotometry. Time scan experiments furnished the reaction rate of sorbitol assayed solution as well as sorbitol-caffeine complex as 0.021 min −1 and 0.018 min −1 , respectively. A sudden drop was observed in the fluorescence lifetime of reduced nicotinamide adenine dinucleotide (NADH) present in sorbitol assayed solution upon complexation with caffeine, that is, from 1.774 × 10 −09 to 1.23 × 10 −10 Sec, which indicates the hindrance in the formation of NADH and the probable formation of some other species. Isothermal titration calorimetric experiments clearly indicate the number of binding sites (i.e., 3.89, 1.40, and 2.07) that exist between sorbitol and caffeine at the complexation ratio of 1:1.2, 1:1.5, and 1:3. The present method can be helpful in pharmacological and therapeutic studies of sorbitol using caffeine for treating diabetic neuropathy.

Biochanin‐A has antidiabetic, antihyperlipidemic, antioxidant, and protective effects on diabetic nephropathy via suppression of TGF‐β1 and PAR‐2 genes expression in kidney tissues of STZ‐induced diabetic rats

“…These disorders eventually lead to micro‐ and macrovascular complications. Diabetic nephropathy (DN) is a serious microvascular complication of diabetes mellitus that affects more than 50% of people 2 . Treatment of DN with dialysis or kidney transplantation is life‐threatening that imposes a heavy cost on patients and society.…”

“…Diabetic nephropathy (DN) is a serious microvascular complication of diabetes mellitus that affects more than 50% of people. 2 Treatment of DN with dialysis or kidney transplantation is life-threatening that imposes a heavy cost on patients and society. The progression of the DN to end-stage renal disease can be blocked by controlling blood glucose and suppressing its progression signaling pathways.…”

Fabrication of In(III)-alizarin red S complex trap for efficient detection of fluoride ion in aqueous environs

“…The interaction between In with ARS and In-ARS complex with F¯ ion was studied using this technique. The thermodynamic parameters like ΔH (change in enthalpy), ΔS (change in entropy) and other parameters like the association constant (K a ) and stoichiometry for the interaction between two or more substances can be obtained from this analysis (Srivastava et al 2020). The instrumentation consists of two cells, viz.…”