Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products

Santos, Édilla Ribeiro dos; Teles, Zozilene Nascimento Santos; Campos, Núria Mariana; Souza, Diogo Angeli Jacinto de; Bispo, Aline Simões da Rocha; Nascimento, Rodrigo Pires do

doi:10.1590/s1516-89132012000500020

Cited by 13 publications

(5 citation statements)

References 22 publications

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“…This strategy resulted in the increase of amylase production (335 IU/L) in 9.25 times. This amylase level is comparable to that described in other studies [ 15 , 16 ] and this demonstrates that the wheat bran is a suitable substrate. The supplementation of wheat bran infusion with peptone decreased the production of amylases.…”

Section: Discussionsupporting

confidence: 90%

Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate

et al. 2014

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The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged to Ascomycota phylum. The strain Aspergillus 6V4 was the best amylase producer in the screening assay of starch hydrolysis in petri dishes (ASHPD) and in the assay in submerged fermentation (ASbF). The strain Aspergillus 6V4 produced high amylase levels (335 UI/L) using wheat bran infusion as the exclusive substrate and the supplementation of this substrate with peptone decreased the production of this enzyme. The moisture content of 70% was the best condition for the production of Aspergillus 6V4 amylases (385 IU/g) in solid state fermentation (SSF).

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Section: Discussionsupporting

confidence: 90%

Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate

et al. 2014

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“… CS Casein (Sigma-Aldrich) 0.3; Soluble starch 10.0 (Merck); Bacteriological agar (Oxoid) 20.0; KNO 3 2.0; NaCl 2.0; KH 2 PO 4 2.0; MgSO 4 .7H 2 O 0.05; CaCO 3 0.02; FeSO 4 .7H 2 O 0.01 [ 21 ]. YS Yeast extract 1.0 (Oxoid); Soluble starch (Merck) 10.0; Bacteriological agar (Oxoid) 20.0; NaNO 3 1.2; KH 2 PO 4 3.0; K 2 HPO 4 6.0; MgSO 4 .7H 2 O 0.2; CaCl 2 .2H 2 O 0.05; MnSO 4 .7H 2 O 0.01; ZnSO 4 .7H 2 O 0.001 [ 22 ]. CYS Casein (Sigma-Aldrich) 0.3; Yeast extract 1.0 (Oxoid); Soluble starch 10.0 (Merck); Bacteriological agar (Oxoid) 20.0; KNO 3 2.0; NaCl 2.0; KH 2 PO 4 2.0; MgSO 4 .7H 2 O 0.05; CaCO 3 0.02; FeSO 4 .7H 2 O 0.01 [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Induction of amylase and protease as antibiofilm agents by starch, casein, and yeast extract in Arthrobacter sp. CW01

2021

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Background In unfavourable environment, such as nutrient limitation, some bacteria encased themselves into a three dimensional polymer matrix called biofilm. The majority of microbial infections in human are biofilm related, including chronic lung, wound, and ear infections. The matrix of biofilm which consists of extracellular polymeric substances (EPS) causes bacterial colonization on medical implanted device in patients, such as catheter and lead to patient’s death. Biofilm infections are harder to treat due to increasing antibiotic resistance compared to planktonic microbial cells and escalating the antibiotic concentration may result into in vivo toxicity for the patients. Special compounds which are non-microbicidal that could inhibit or destroy biofilm formation are called antibiofilm compounds, for example enzymes, anti-quorum sensing, and anti-adhesins. Arthrobacter sp. CW01 produced antibiofilm compound known as amylase. This time our preliminary study proved that the antibiofilm compound was not only amylase, but also protease. Therefore, this research aimed to optimize the production of antibiofilm agents using amylase and protease inducing media. The five types of production media used in this research were brain heart infusion (BHI) (Oxoid), BHI with starch (BHIS), casein with starch (CS), yeast extract with starch (YS), and casein-yeast extract with starch (CYS). Biofilm eradication and inhibition activities were assayed against Pseudomonas aeruginosa (ATCC 27,853) and Staphylococcus aureus (ATCC 25,923). Results The results showed that different production media influenced the antibiofilm activity. Addition of starch, casein and yeast extract increased the production of amylase and protease significantly. Higher amylase activity would gradually increase the antibiofilm activity until it reached the certain optimum point. It was shown that crude extracts which contained amylase only (BHI, BHIS and YS) had the optimum eradication activity against P. aeruginosa and S. aureus biofilm around 60–70 %. Meanwhile, CS and CYS crude extracts which contained both amylase and protease increased the biofilm eradication activity against both pathogens, which were around 70–90 %. Conclusions It was concluded that the combination of amylase and protease was more effective as antibiofilm agents against P. aeruginosa and S. aureus rather than amylase only.

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“…strains SO6, PM1, and PM7 presented a high biochemical activity, being positive for at least 12 of the 13 enzymes tested. Moreover, the three preselected strains were capable of fermenting sugars and showed proteolytic and amylolytic activity, with the latter related with the conversion of starch into shorter polymers of glucose units [ 48 ]. The biochemical characteristics of Streptomyces spp.…”

Section: Discussionmentioning

confidence: 99%

Bioconversion of potato solid waste into antifungals and biopigments using Streptomyces spp.

et al. 2021

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Potato waste was processed and used as a sole substrate for simultaneously producing antifungals and biopigments using Streptomyces spp. Out of three different Streptomyces isolates, strain SO6 stood out due to its ability to produce antifungals against economically important fungal phytopathogens and intracellular biopigments using potato waste powders without additional nutrients. This strain also showed the potential to secrete a broad range of enzymes for fermentation of eight sugars that could be involved in potato waste bioconversion. The results of the fermentation assay indicated that Streptomyces sp. strain SO6 degrades potato wastes during submerged fermentation, diminishing total dry weight and increasing reducing sugars from 0.3 to 3.6 mg·mL−1 and total proteins from 70.6 to 187.7 μg·mL−1. The results showed that Streptomyces strain SO6 was able to convert the potato waste into 0.96 mg·g−1 of diffusible antifungals and 1.75 mg·g−1 of reddish-purple biopigments. On the contrary, an absence of pigment production was observed during the fermentation of the commercial medium used as reference. According to our results, replacement of commercial culture media with available low-cost agroindustrial wastes for producing bioactive chemicals is a real opportunity to enhance the Streptomyces pigment production and antibiotic sustainability with cost-competitiveness. To our knowledge, this is the first report on the simultaneous production of biopigments and diffusible antifungal antibiotics produced by Streptomyces spp. using potato solid waste as the sole nutrient source.

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Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products

Abstract: ABSTRACT

Cited by 13 publications

References 22 publications

Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate

Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate

Induction of amylase and protease as antibiofilm agents by starch, casein, and yeast extract in Arthrobacter sp. CW01

Bioconversion of potato solid waste into antifungals and biopigments using Streptomyces spp.

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