Isolation and screening of microorganisms with potential for biotransformation of terpenic substrates

Bier, Mário César Jucoski; Poletto, Sabrina; Soccol, Vanete Thomaz; Soccol, Carlos Ricardo; Medeiros, Adriane Bianchi Pedroni

doi:10.1590/s1516-89132011000500020

Cited by 12 publications

(4 citation statements)

References 24 publications

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“…The mycelia were washed with saline water and then re-suspended into the same volume of M9 medium. Limonene (1%, v/v ) was added and cultures were incubated for 6 d. The biomass was filtered and quantified by dry weight to verify the growth of the microorganisms [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…Recently, many researches have reported that many strains (fungi, bacteria and yeasts) could yield aroma compounds using limonene as the precursor [ 9 , 14 , 15 , 16 ]. However, the limonene biotransformation process is still limited because of the high volatility and low solubility of this precursor, the low transformation rates, high cytotoxicity of both precursor and product, and so on [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Screening a Strain of Klebsiella sp. O852 and the Optimization of Fermentation Conditions for Trans-Dihydrocarvone Production

et al. 2021

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Flavors and fragrances have high commercial value in the food, cosmetic, chemical and pharmaceutical industries. It is interesting to investigate the isolation and characterization of new microorganisms with the ability to produce flavor compounds. In this study, a new strain of Klebsiella sp. O852 (accession number CCTCC M2020509) was isolated from decayed navel orange (Citrus sinensis (L.) Osbeck), which was proved to be capable of converting limonene to trans-dihydrocarvone. Besides, the optimization of various reaction parameters to enhance the trans-dihydrocarvone production in shake flask was performed for Klebsiella sp. O852. The results showed that the yield of trans-dihydrocarvone reached up to 1 058 mg/L when Klebsiella sp. O852 was incubated using LB-M medium for 4 h at 36 °C and 150 rpm, and the biotransformation process was monitored for 36 h after adding 1680 mg/L limonene/ethanol (final ethanol concentration of 0.8% (v/v)). The content of trans-dihydrocarvone increased 16 times after optimization. This study provided a basis and reference for producing trans-dihydrocarvone by biotransformation.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Screening a Strain of Klebsiella sp. O852 and the Optimization of Fermentation Conditions for Trans-Dihydrocarvone Production

et al. 2021

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“…Substrate toxicity was performed to screen monoterpene-resistant microorganisms in accordance with previous methods. 12,13 The culture plates were prepared by displacing 30 ml sterilized NAM in pre-sterilized Petri dishes. Each 1 ml (1.0 x 10 5 CFU/ml) inoculum is evenly distributed to the agar medium with a sterile glass rod.…”

Section: Substrate-toxicity Testmentioning

confidence: 99%

Screening of Microorganisms Capable of Biotransforming Certain Monoterpenes Using Substrate Toxicity Test

Mittal,

Srivastava,

Ganjewala

2024

J. Pure Appl. Microbiol.

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Monoterpenes, such as Geraniol (G), Geranyl acetate (GA), Citral (CT), Limonene (LN), and Linalool (LL), are the most widely used phytochemicals in the aroma, food, and pharmaceutical industries. Here, we screened several bacteria and fungi to assess their potential to biotransform the selected monoterpenes (G, GA, CT, LN, and LL) through the substrate toxicity test. Three bacteria Pseudomonas fluorescens MTCC2421, Streptococcus mutans MTCC497, and Escherichia coli were found to be resistant to G, GA, and LN while two P. aeruginosa, and S. epidermidis MTTC 435 to GA and LN. In general, all fungal strains did not show resistance to any of the monoterpenes used, except Candida albicans and Fusarium oxysporum, which were slightly resistant to lower concentrations (0.05-0.1%) of GA. Interestingly, none of the bacteria and fungi showed any resistance to CT. The maximum concentrations of monoterpenes to which bacteria exhibited resistance ranged from 0.05-0.2%. The growth and biomass profiles of bacteria revealed that P. fluorescens and S. mutans grew well in the presence of monoterpenes GA and LN. Based on this, Pseudomonas fluorescens was capable of biotransforming GA and LN, while S. mutans only LN. The biotransformation of GA by P. fluorescens produced G and LL on the day 5th and 7th of the incubation. Hence, the study revealed the three potential bacteria, which may be useful in producing new aromatic derivatives from selected monoterpenes through biotransformation.

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“…To this end, researchers have engineered organisms for its production as well as utilized limonene as a substrate for biotransformation to generate products such as limonene-1,2-diol and α-terpineol (Bier et al., 2017 ; Sales et al., 2018 ; Tai et al., 2016 ). Ongoing studies into the mechanism of limonene toxicity have identified organisms with natural tolerance to limonene (Lennartsson et al., 2012 ) and even some capable of utilizing limonene as a sole carbon source (Bier et al., 2011 ; Sales et al., 2018 ). Likewise, enhanced tolerance to limonene is possible via adaptive laboratory evolution, and rationally engineering genes identified via mutant screening, transporter libraries, and transcriptomics (Chubukov et al., 2015 ; Dunlop et al., 2011 ; Li et al., 2021 ; Ren et al., 2020 ).…”

Section: Carbohydrate-rich Food Wastesmentioning

confidence: 99%

Microbial valorization of underutilized and nonconventional waste streams

Lad

Coleman

Alper

2021

Journal of Industrial Microbiology and Biotechnology

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The growing burden of waste disposal coupled with natural resource scarcity has renewed interest in the remediation, valorization and/or repurposing of waste. Traditional approaches such as composting, anaerobic digestion, use in fertilizers or animal feed, or incineration for energy production extract very little value out of these waste streams. In contrast, waste valorization into fuels and other biochemicals via microbial fermentation is an area of growing interest. In this review, we discuss microbial valorization of nonconventional, aqueous waste streams such as food processing effluents, wastewater streams, and other industrial wastes. We categorize these waste streams as carbohydrate-rich food wastes, lipid-rich wastes, and other industrial wastes. Recent advances in microbial valorization of these nonconventional waste streams are highlighted, along with a discussion of the specific challenges and opportunities associated with impurities, nitrogen content, toxicity, and low productivity.

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Isolation and screening of microorganisms with potential for biotransformation of terpenic substrates

Abstract: ABSTRACT

Cited by 12 publications

References 24 publications

Screening a Strain of Klebsiella sp. O852 and the Optimization of Fermentation Conditions for Trans-Dihydrocarvone Production

Screening a Strain of Klebsiella sp. O852 and the Optimization of Fermentation Conditions for Trans-Dihydrocarvone Production

Screening of Microorganisms Capable of Biotransforming Certain Monoterpenes Using Substrate Toxicity Test

Microbial valorization of underutilized and nonconventional waste streams

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