Evaluation of cytotoxicity and biological activities in extracellular polysaccharides released by cyanobacterium Arthrospira platensis

Abstract

The aim of this work was to evaluate the cytotoxicity of Arthrospira platensis Extracellular Polymeric Substances (EPS) for colon cancer and kidney cell lines. Results showed that EPS were free from cytotoxic effects. A variety of solvents were assessed for their ability to extract the bioactive ingredients from EPS. Methanol gave the highest yield (75.75%) than other solvents. The extracts were tested for activities against a collection of Gram+/- bacteria. The methanol extract exhibited a more potent activity than the other organic extracts, whereas the aqueous extract was active against Staphylococcus epidermis (Gram+) and Salmonella typhimurium (Gram-). Finally, The extracts were also tested for the antioxidant activity, using the Trolox Equivalent Antioxidant Activity assay. The methanol extract displayed a moderate antioxidant activity (TEAC = 0.027 mg/ml). The HPLC analysis of this extract revealed two distinct peaks: 8.1 kDa (8.31 min) and 4.1 kDa (8.54 min)

Antioxidant and Antimicrobial Activities of Exopolysaccharides from Yoghurt Starter

“…For [11] also screened the antibacterial activities of different extracts of the EPS (Methanol, Hexane, Petroleum ether, Ethanol, Acetone, and Water) of Cyanobacterium Arthrospira platensis against a collection of Gram+/-bacteria by the paper disk agar diffusion method, the determination of Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC). The methanol extract exhibited a more potent activity than the other organic extracts, whereas the aqueous extract was active against Staphylococcus epidermis (Gram+) and Salmonella typhimurium (Gram-).…”

“…(2007) [23] observed the antioxidant and free radical scavenging activities of exopolysaccharide isolated from Rhizobium meliloti. A moderate antioxidant activity of methanol extract of exopolysaccharide from Cyanobacterium Arthrospira platensis using the Trolox Equivalent Antioxidant Activity assay was determined by Challouf et al (2011) [11]. A strong scavenging ability of exopolysaccharide from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315 was observed against DPPH and superoxide radicals at high concentration [15].…”

Value added by Spirulina platensis in two different diets on growth performance, gut microbiota, and meat quality of Japanese quails

“…Spirulina can be considered as feed supplement that has many health benefits for humans, and a feed additive for animals having economic benefits [17]. SP is one of the most important micro-alga showing antimicrobial activity against many pathogenic bacteria and fungi [19] and it contains active ingredients such as tocopherols, C-phycocyanin, and extracellular polysaccharide [20-22] which have antimicrobial activities against Escherichia coli , Pseudomonas sp., Enterobacter sp., Salmonella Typhi ; Klebsiella pneumoniae , and Proteus vulgaris [23-26]. Staphylococcus aureus ; Staphylococcus epidermis and Aeromonas liquefaciens [27, 28].…”

Effects of flask configuration on biofilm growth and metabolites of intertidal Cyanobacteria isolated from a mangrove forest

“…The isocratic chromatographic separation parameters were-detection: PDA 254 nm; mobile phase: mixture of acetonitrile 60%, water 40%, flow rate of 1 ml min À1 . The total run time was 45 min (Challouf et al 2011;Afreen et al 2017).…”