Effects of flask configuration on biofilm growth and metabolites of intertidal Cyanobacteria isolated from a mangrove forest

Maruthanayagam, Veerabadhran; Chakraborty, Sandeep; Mitra, Sudip; Karmakar, Subrata; Mukherjee, Joydeep

doi:10.1111/jam.13761

Cited by 14 publications

(8 citation statements)

References 78 publications

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“…While it is accepted that the true ecological role of many microbial SMs has not been experimentally validated, antibiotic production has long been believed to be involved in competitive inhibition of the growth of proximal microbes or possibly in intercellular signaling. The Mukherjee group reported several findings connecting vessel types, lifestyles, and SM production in various microorganisms [ 79 , 80 , 81 ]. For example, Mitra and colleagues designed a conico-cylindrical flask (CCF) with an ultra-low-speed rotating disk bioreactor [ 79 ].…”

Section: Change In Physical Parametersmentioning

confidence: 99%

“…AP3b, and Chroococcus sp. AP3U [ 81 ]. Upon comparing the metabolite profiles of these strains, grown in biofilms in the CCF-PMMA, to those of the same strains grown as plankton in a standard EF; they found that biofilms formed in the CCF but not in the EF and that cell-bound polysaccharide and released polysaccharide production was higher in CCF than in EF.…”

Section: Change In Physical Parametersmentioning

confidence: 99%

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Extending the “One Strain Many Compounds” (OSMAC) Principle to Marine Microorganisms

et al. 2018

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Genomic data often highlights an inconsistency between the number of gene clusters identified using bioinformatic approaches as potentially producing secondary metabolites and the actual number of chemically characterized secondary metabolites produced by any given microorganism. Such gene clusters are generally considered as “silent”, meaning that they are not expressed under laboratory conditions. Triggering expression of these “silent” clusters could result in unlocking the chemical diversity they control, allowing the discovery of novel molecules of both medical and biotechnological interest. Therefore, both genetic and cultivation-based techniques have been developed aimed at stimulating expression of these “silent” genes. The principles behind the cultivation based approaches have been conceptualized in the “one strain many compounds” (OSMAC) framework, which underlines how a single strain can produce different molecules when grown under different environmental conditions. Parameters such as, nutrient content, temperature, and rate of aeration can be easily changed, altering the global physiology of a microbial strain and in turn significantly affecting its secondary metabolism. As a direct extension of such approaches, co-cultivation strategies and the addition of chemical elicitors have also been used as cues to activate “silent” clusters. In this review, we aim to provide a focused and comprehensive overview of these strategies as they pertain to marine microbes. Moreover, we underline how changes in some parameters which have provided important results in terrestrial microbes, but which have rarely been considered in marine microorganisms, may represent additional strategies to awaken “silent” gene clusters in marine microbes. Unfortunately, the empirical nature of the OSMAC approach forces scientists to perform extensive laboratory experiments. Nevertheless, we believe that some computation and experimental based techniques which are used in other disciplines, and which we discuss; could be effectively employed to help streamline the OSMAC based approaches. We believe that natural products discovery in marine microorganisms would be greatly aided through the integration of basic microbiological approaches, computational methods, and technological innovations, thereby helping unearth much of the as yet untapped potential of these microorganisms.

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Section: Change In Physical Parametersmentioning

confidence: 99%

Section: Change In Physical Parametersmentioning

confidence: 99%

Extending the “One Strain Many Compounds” (OSMAC) Principle to Marine Microorganisms

et al. 2018

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“…Benthic biofilms are highly adapted to changing environmental conditions (dramatic changes in environmental parameters and nutrient concentrations) and are absorbers of excess nutrients from the environment [ 77 ]. The trichomes of the Baikal strain tightly intertwine and form a biofilm, characteristic of many species of Oscillatoriales cyanobacteria, in which the bacteria are held together by a polysaccharide mucus that is able to withstand harsh environmental conditions that might involve nutrient deficiencies and physical stress [ 78 ]. Various types of microscopy have been used to study the morphology of biofilms.…”

Section: Discussionmentioning

confidence: 99%

Tychonema sp. BBK16 Characterisation: Lifestyle, Phylogeny and Related Phages

Evseev

Тихонова

Krasnopeev

et al. 2023

Viruses

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Cyanobacterial expansion is harmful to the environment, the ecology of Lake Baikal and the economy of nearby regions and can be dangerous to people and animals. Since 2011, the process of colonisation of the lake with potentially toxic cyanobacteria belonging to the genus Tychonema has continued. An understanding of the mechanism of successful expansion of Tychonema requires scrutiny of biological and genomic features. Tychonema sp. BBK16 was isolated from the coastal zone of Lake Baikal. The morphology of BBK16 biofilm was studied with light, scanning electron and confocal microscopy. The biofilm is based on filaments of cyanobacteria, which are intertwined like felt; there are also dense fascicles of rope-like twisted filaments that impart heterogeneity to the surface of the biofilm. Genome sequencing, intergenomic comparisons and phylogenetic analyses indicated that Tychonema sp. BBK16 represent a new species related to planktic cyanobacterium Tychonema bourrellyi, isolated from Alpine lentic freshwater. Genome investigation revealed the genes possibly responsible for the mixotrophic lifestyle. The presence of CRISPR-Cas and restriction modification defence mechanisms allowed to suggest the existence of phages infecting Tychonema sp. BBK16. Analysis of CRISPR spacers and prophage-derived regions allowed to suggest related cyanophages. Genomic analysis supported the assumption that mobile elements and horizontal transfer participate in shaping the Tychonema sp. BBK16 genome. The findings of the current research suggest that the aptitude of Tychonema sp. BBK16 for biofilm formation and, possibly, its mixotrophic lifestyle provide adaptation advantages that lead to the successful expansion of this cyanobacterium in the Baikal’s conditions of freshwater lake environments.

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“…In a previous study, increased levels of capsular/ bound polysaccharides were observed when fermentation of Leptolyngbya sp. was carried out in biofilm culture in conicocylindrical flasks compared to culture in planktonic mode in Erlenmeyer flasks (Veerabadhran et al 2018). Metabolomics can also be used to analyze the behavior of cyanobacterial strains subjected to stress conditions.…”

Section: Introductionmentioning

confidence: 99%

Heat shock and iron limitation modulate the metabolic profile of the cyanobacterium Microcystis aeruginosa NIES‐88

Balloo

Mehjabin

Phan

et al. 2023

Phycological Research

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The freshwater cyanobacterium Microcystis aeruginosa NIES‐88, which can produce microcystins, micropeptins, and argicyclamides, was subjected to a one strain many compounds (OSMAC) analysis. We report its response to two environmental stressors, temperature and iron limitation, by means of untargeted and targeted metabolomics. The results demonstrated a slower specific growth rate of 0.20 per day and 0.16 per day in adverse conditions of 37°C and iron limitation, respectively. The metabolic signature of M. aeruginosa was highly dependent on incubation temperatures. Production of microcystins LR and RR was severely downregulated while that of argicyclamide B was significantly upregulated, with a highest 10‐fold increase on day 14 of heat shock treatment. M. aeruginosa NIES‐88 was found to produce a new compound, argicyclamide D (1), in iron limited medium, which has the same macrocyclic structure as the previously reported analogs. Hence, it is proposed that acclimation of M. aeruginosa to environmental stressors might be mediated by a change in the metabolic pathways as well as modulation of the levels of their expressed metabolites.

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Effects of flask configuration on biofilm growth and metabolites of intertidal Cyanobacteria isolated from a mangrove forest

Abstract: The present study augmented the information which can enhance wider exploration of the biofilm mode of cultivation of Cyanobacteria.

Cited by 14 publications

References 78 publications

Extending the “One Strain Many Compounds” (OSMAC) Principle to Marine Microorganisms

Extending the “One Strain Many Compounds” (OSMAC) Principle to Marine Microorganisms

Tychonema sp. BBK16 Characterisation: Lifestyle, Phylogeny and Related Phages

Heat shock and iron limitation modulate the metabolic profile of the cyanobacterium Microcystis aeruginosa NIES‐88

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