Antioxidant and Antimicrobial Activities of Exopolysaccharides from Yoghurt Starter

Ghalem, Bachir Raho

doi:10.11648/j.ab.20170505.13

Cited by 13 publications

(11 citation statements)

References 19 publications

(24 reference statements)

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“…This result is in agreement with Zhang et al [26] in which it was reported that EPS produced by Lactobacillus plantarum showed a DPPH scavenging activity of 52.23% at 1000 µg/mL. Similarly, Ghalem [27] documented a DPPH radical scavenging of 24.25% from EPS produced by yoghurt starter culture. In contrast, Xu et al [28] reported that the DPPH radical scavenging activity of the EPS isolated from Bifidobacterium animalis could increase with the increasing EPS concentration and reach to a similar activity with ascorbic acid.…”

Section: Dpph Free Radical Scavenging Activity Of the Epssupporting

confidence: 92%

Antioxidant Activities of Exopolysaccharides Produced by Lactic Acid Bacteria Isolated from Commercial Yoghurt Samples

Adelekan¹,

Olurin²,

Ezeani³

2020

AiM

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An antioxidant is a substance that inhibits the oxidation of other molecules caused by free radicals. The inbuilt antioxidant systems possessed by living organisms are generally not enough to prevent them from oxidative damages and the uses of synthetic antioxidants also have some harmful effects. This study was aimed at evaluating the antioxidant activities of exopolysaccharides produced by lactic acid bacteria isolated from yoghurt. Lactic acid bacteria (LAB) were isolated from six different brands of commercially available yoghurt using deMan Rogosa Sharpe (MRS) agar. The LAB isolates were identified based on morphological and biochemical analyses and were screened for exopolysaccharide (EPS) production. The LAB isolates screened positive were used for EPS production in a liquid medium and the EPS produced were purified and quantified using standard methods. Antioxidant activities of the EPS were evaluated by determining the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferric ion reducing power, and total phenolic contents. Data obtained were analysed using Analysis of Variance. Total lactic acid bacterial count obtained from the yoghurt samples ranged from 0-3.9 × 10 4 CFU/mL with sample A (Fan Yoghurt) having the highest LAB count (3.9 × 10 4 CFU/mL). The isolated LAB and their incidence rate were Lactobacillus plantarum (25.49%), L. delbrueckii (19.61%), L. fermentum (15.69%), L. acidophilus (13.73%), Leuconostoc mesenteroides (11.76%), Lactococcus lactis (7.84%), and Lactobacillus casei (5.88%). Fifty-one out of the 64 LAB isolates were screened positive for EPS production and only six were able to produce substantial quantity of EPS ranging from 127.4-208.5 mg/L. The exopolysaccharides produced by L. fermentum had the highest DPPH radical scavenging activity (62.90%) while that of L. plantarum had the lowest (23.10%) at a concentration of 1000 µg/mL. Also, the EPS produced by L. fermentum recorded the highest ferric ion reducing power (12.89 mg AAE/mL) at 1000 µg/mL while that of L. plantarum had the lowest (5.62 mg AAE/mL). At 1000 How to cite this paper: Adelekan, A.O., Olurin, T.O. and Ezeani, A.O. (2020) Antioxidant Activities of Exopolysaccharides Produced by Lactic Acid Bacteria Isolated from Commercial Yoghurt Samples. Advances in Microbiology,

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Section: Dpph Free Radical Scavenging Activity Of the Epssupporting

confidence: 92%

Antioxidant Activities of Exopolysaccharides Produced by Lactic Acid Bacteria Isolated from Commercial Yoghurt Samples

Adelekan¹,

Olurin²,

Ezeani³

2020

AiM

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“…Findings from this study also support observation by Nyanzi, Shuping, Jooste, and Eloff (2015) contributed by exopolysaccharides (EPS) (Ghalem, 2017;Zhang et al, 2012).…”

Section: Discussionsupporting

confidence: 87%

“…The antioxidative activity of Lactobacillus spp. is mostly contributed by exopolysaccharides (EPS) (Ghalem, ; Zhang et al., ).…”

Section: Resultsmentioning

confidence: 99%

Probiotic potentials of Lactobacillus plantarum isolated from fermented durian (Tempoyak), a Malaysian traditional condiment

Ahmad

Yap

Kofli

et al. 2018

Food Science & Nutrition

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Lactic acid bacterium isolated from fermented durian (tempoyak) was investigated for its potentials as a probiotic strain. Bacterial tolerance toward gastrointestinal environment, adhesion, and cytotoxic activity in human colon adenocarcinoma cell line HT‐29 was evaluated. 16S rRNA sequencing identified the lactic acid bacterium as Lactobacillus plantarum. The bacterium demonstrated good tolerance toward gastrointestinal pH 2.0 and 0.3% bile salts. It showed strong adhesive capacity in human intestinal cell line, HT‐29, with an adhesion index of 159 ± 10. Cytotoxicity of L. plantarum was investigated using both live bacterial cells (BC) and cell‐free supernatant (CFS). Findings showed that both BC and CFS of L. plantarum reduced proliferation of HT‐29 colon adenocarcinoma cells using MTT assay. The results imply potential probiotic properties of L. plantarum isolated from tempoyak.

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“…In this work, microorganisms isolated from seawater of Mauritius were screened for their ability to produce polysaccharides which exhibit antibacterial properties. Several authors have reported on the potential of exopolysaccharides as antimicrobial agents (Wilson et al 2011;Karlapudi et al 2016;Wu et al 2016) and this property was demonstrated for EPS obtained from different types of microorganisms (Ghalem 2017;Jeong et al 2017). Similarly, in this study, eight selected marine isolates were shown to produce EPS which could successfully inhibit the growth of human pathogenic bacteria.…”

Section: Discussionsupporting

confidence: 61%

“…More recently, biological activities of exopolysaccharides have been reported, with a number of recent studies documenting their immunostimulatory, antiviral, anti-oxidative, anti-tumour and antibacterial properties (Balzaretti et al 2017;Zhou et al 2017;Reichert et al 2017;El-Deeb et al 2018;Wen et al 2018). The latter represents an interesting prospect in view of the increasing resistance of pathogenic microorganisms to existing antibiotics (Ghalem 2017). Indeed, drug-resistant microorganisms have become an increasing concern and the potential of microbial exopolysaccharides in fighting microbial resistance lies both in their intrinsic biological activities and their ability to be used in drug delivery systems (El-Naggar et al 2016;Sezer et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetics and antibacterial properties of exopolysaccharides from marine bacteria isolated from Mauritius seawater

et al. 2019

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Purpose The marine environment harbours diverse bacterial species which can be exploited for the production of valuable compounds such as exopolysaccharides (EPS) which hold promises for biotechnological applications. The coastal waters of Mauritius is a relatively underexplored marine environment and in this study, isolated bacterial species were tested for the production of EPS exhibiting antibacterial properties against human bacterial pathogens from the genera Acinetobacter, Bacillus, Campylobacter, Enterobacter, Enterococcus, Escherichia, Proteus, Pseudomonas, Salmonella, Streptococcus and Staphylococcus. Methods Bacteria were first isolated from seawater samples. Using the disc diffusion method, their EPS were tested for antibacterial effects through two screenings, with each involving a different set of arbitrarily chosen group of pathogens. The microorganisms producing antibacterial EPS were subsequently identified by morphological, biochemical and 16S rRNAbased phylogenetic analyses. Those EPS exhibiting broadest antibacterial activities were eventually characterised by Fouriertransform infrared spectroscopy (FTIR) and thin-layer chromatography (TLC). Results Eight EPS were found to display antibacterial effects against more than half of the pathogens and the microorganisms producing them were identified as Bacillus, Halomonas, Psychrobacter and Alcaligenes species. However, only two of these EPS were found to be the most active, with their MIC values ranging between 62.5 and 500 μg/ml. FTIR and TLC analyses revealed the presence of carboxyl, hydroxyl and amide as well as sulphate for the EPS, with glucose or fructose being the main sugar. Conclusion The results suggest that Mauritius seawater can be a source of biotechnologically useful microorganisms, producing EPS having potential as antimicrobial agents. DNA sequence data also suggest possible novel bacterial species.

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Antioxidant and Antimicrobial Activities of Exopolysaccharides from Yoghurt Starter

Cited by 13 publications

References 19 publications

Antioxidant Activities of Exopolysaccharides Produced by Lactic Acid Bacteria Isolated from Commercial Yoghurt Samples

Antioxidant Activities of Exopolysaccharides Produced by Lactic Acid Bacteria Isolated from Commercial Yoghurt Samples

Probiotic potentials of Lactobacillus plantarum isolated from fermented durian (Tempoyak), a Malaysian traditional condiment

Phylogenetics and antibacterial properties of exopolysaccharides from marine bacteria isolated from Mauritius seawater

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