Fusarium verticillioides strains isolated from corn feed: characterization by fumonisin production and RAPD fingerprinting

Ono, Elisabete Yurie Sataque; Fungaro, Maria Helena Pelegrinelli; Sofia, Silvia Helena; Miguel, Tatiana de Ávila; Sugiura, Yoshitsugu; Hirooka, Elisa Yoko

doi:10.1590/s1516-89132010000400026

Cited by 10 publications

(10 citation statements)

References 30 publications

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“…In the present work, variation in fumonisin levels produced by different Fusarium isolates is consistent with other reports (Covarelli et al, 2012;Rocha et al, 2011;Ono et al, 2010;Picot et al, 2010;Nelson et al, 1991).…”

Section: Discussionsupporting

confidence: 93%

“…However, in the present study the period of incubation was 14 days, according to the methodology proposed by Ono et al (2010), whereas in the work carried out by Nelson et al (1991) and Rocha et al (2011) the period of incubation was 30 days.…”

Section: Discussionmentioning

confidence: 49%

“…Thereafter, four mycelial discs (0.5 cm diameter) of each culture were inoculated in 40 g of ground corn previously sterilized and moistened with 20 ml of deionized water, and incubated at 27 C for 14 days under 12 h photoperiod as described by Ono et al (2010). Then, the cultures were transferred to paper bags and placed in a forced-air ventilation oven at 65 C for 48 h to dry.…”

Section: Methodsmentioning

confidence: 99%

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Prevalence of fumonisin-producing Fusarium species in Brazilian corn grains

et al. 2014

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 49%

Section: Methodsmentioning

confidence: 99%

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Prevalence of fumonisin-producing Fusarium species in Brazilian corn grains

et al. 2014

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“…Para ahli taksonomi menyatakan bahwa spesies F. verticillioides merupakan sinonim F. moniloforme dan dominan menginfeksi jagung (Oren et al 2003;Ono et al 2010;Stumpf et al 20013). Di Indonesia, penelitian cemaran mikotoksin dari F. verticillioides dan tingkat toleransinya dalam pakan ternak belum banyak dilaporkan.…”

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Cemaran Mikotoksin, Bioekologi Patogen Fusarium veriticillioides dan Upaya Pengendaliannya pada Jagung

Pakki

2016

J. Litbang Pert.

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ABSTRAKFusarium sp. merupakan salah satu patogen penting pada tanaman jagung di Indonesia, yang menginfeksi batang, tongkol, dan biji jagung di lapangan maupun pada tempat penyimpanan. Cemaran F. verticillioides perlu diwaspadai karena patogen tersebut menghasilkan toksin fumonisin (FB1, FB2, dan FB3). Fusarium sp. terdiri atas enam spesies, dan spesies yang dominan menginfeksi jagung ialah F. verticillioides. Infeksi patogen tersebut pada biji jagung dapat menimbulkan gejala maupun tanpa gejala (symptomless). Pengendalian hayati pada tanaman di lapangan dengan Bacillus amyloliquefaciens, B. mojavensis, dan bahan kimia berbahan aktif asam amonia dan propionat efektif menekan infeksi F. verticillioides dan selanjutnya mengurangi kontaminasi fumonisin di tempat penyimpanan. Pengendalian patogen di tempat penyimpanan dapat dilakukan melalui sanitasi dan aplikasi propionat, menyimpan biji jagung dengan kadar air 1213%, serta menjaga suhu dan kelembapan tetap rendah.

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“…RAPD-genetic characterisation of Fusarium species from animal feed have already been carried out based on fumonisin production. (Ono et al, 2010;Yazeed et al, 2011;Daie-Ghazvini et al, 2011). Considering the importance of rice regarding food security in India genetic diversity of Fusarium isolates need to be explored from the view point of fumonisin production for setting up management strategies.…”

mentioning

confidence: 99%

Molecular detection and genetic characterisation of fumonisin producingfusariumisolates from rice cultivars

Nayak¹,

Dhua²,

Samanta³

et al. 2014

Intern. Jour. of Agricul., Environ. and Biotech.

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Fusarium species mainly produce fumonisins group of mycotoxins which are classified as Group 2B human carcinogen by International Agency for Research on Cancer (IARC). In poor storage conditions, Fusarium species producing fumonisins can infect rice or paddy (Oryza sativa L.) which is the highest produced and consumed staple food in India. A rapid molecular method using primer Fum5F and Fum6R detected 85% fumonisin producers among 28 Fusarium isolates from Indian rice cultivars. Genetic variability of the isolates was studied by PCR based RAPD assay using 13 random primers. A total of 169 polymorphic bands were obtained by 13 markers with an average polymorphism information content (PIC) of 0.665 and overall polymorphism of 88%. Primer 3B showed a polymorphism of 96% with PIC value of 0.66 and it amplified 26 scorable fragments hence may be useful for the analysis of genetic variation among Fusarium isolates. Four strains (F47, F90, F92 and F96) in which fum gene wasn't amplified by Fum5F and Fum6R and supposed to be non producer of fumonisin have been consistently placed in one separate group by RAPD primers. Genetic variation of toxic Fusarium in rice from India is less studied. RAPD proved to be a suitable tool for depicting Polymorphism among the isolates. The high genetic variability among the Fusarium isolates used in the current study is a matter of concern considering the importance of Rice in India. Highlights• Fumonisins are Group 2B human carcinogen produced by Fusarium species.• Fum5F and Fum6R primer pair detected fumonisin producers.• RAPD primers deciphered 88% polymorphism among 26 Fusarium isolates.

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Fusarium verticillioides strains isolated from corn feed: characterization by fumonisin production and RAPD fingerprinting

Abstract: In this study a total of 16 Fusarium verticillioides strains isolated from corn

Cited by 10 publications

References 30 publications

Prevalence of fumonisin-producing Fusarium species in Brazilian corn grains

Prevalence of fumonisin-producing Fusarium species in Brazilian corn grains

Cemaran Mikotoksin, Bioekologi Patogen Fusarium veriticillioides dan Upaya Pengendaliannya pada Jagung

Molecular detection and genetic characterisation of fumonisin producingfusariumisolates from rice cultivars

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