Prevalence of fumonisin-producing Fusarium species in Brazilian corn grains

Lanza, F.; Zambolim, Laércio; Costa, R. V. da; Queiróz, Valéria Aparecida Vieira; Souza, André Luis Santos de; Figueiredo, J. E. F.

doi:10.1016/j.cropro.2014.08.003

Cited by 29 publications

(19 citation statements)

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“…In this research, the majority of the strains isolated from maize grains in Italy, Spain, Tunisia and Iran, analyzed in this study, produced detectable levels of fumonisins (91%, 100%, 94% and 94% respectively; Figure 2), while the remaining part showed a lack of ability to produce measurable amounts of these mycotoxins. Similar percentages of fumonisin-producing strains (> 80%) were also detected in other F. verticillioides populations isolated from maize in Croatia [68], Spain [15,69], Italy [50], Iran [22], Egypt [17], Brazil [41,44,49], Korea [70], USA [71], Argentina [55,72] and from durum wheat in Argentina [2].…”

Section: Discussionsupporting

confidence: 72%

“…For this reason, the European Commission has established maximum limits for the sum of FB 1 and FB 2 in maize for human consumption [36,37].The amount of fumonisins found in maize kernels is also dependent on the toxigenic ability of the F. verticillioides populations occurring in a certain cultivated field or in a specific geographic area [38]. In fact, within the F. verticillioides species, fumonisin production commonly varies quantitatively because of the different strain abilities to biosynthesize different levels of these mycotoxins [15,24,[39][40][41]. The amount of fumonisins produced may also vary in quantity depending on substrate [42], biotic and abiotic factors [43] as well as on the relative expression of the genes involved in the biosynthetic pathway [44].…”

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confidence: 99%

“…This can provide an important insight on the relationships, the variations and/or the similarities among strains originating from various regions as well as on the possible correlations between genetic variability and different fumonisin production [38,[51][52][53][54]. Analyses of fumonisin biosynthesis and/or molecular characterization of F. verticillioides strains have been conducted in populations from different countries such as Argentina [55], Brazil [38,41,44,49], Italy [50], Iran [22,52], Ethiopia [53] and Nigeria [54].A similar approach was adopted in the present work to characterize selected F. verticillioides strains originating from five Mediterranean countries to simultaneously compare them in a wider geographical context by evaluating their in vitro fumonisin production and genetic profile. Specifically, the main objectives of the present study were to: (i) investigate the abilities of selected F. verticillioides strains isolated from maize kernels in five Mediterranean countries to in vitro biosynthesize FB 1 , FB 2 and FB 3 ; (ii) characterize the genetic structure of these selected strains to assess for possible variability within strains originating from each of the surveyed countries and between the strains originating from different countries.…”

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In Vitro Fumonisin Biosynthesis and Genetic Structure of Fusarium verticillioides Strains from Five Mediterranean Countries

et al. 2020

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Investigating the in vitro fumonisin biosynthesis and the genetic structure of Fusarium verticillioides populations can provide important insights into the relationships between strains originating from various world regions. In this study, 90 F. verticillioides strains isolated from maize in five Mediterranean countries (Italy, Spain, Tunisia, Egypt and Iran) were analyzed to investigate their ability to in vitro biosynthesize fumonisin B 1 , fumonisin B 2 and fumonisin B 3 and to characterize their genetic profile. In general, 80% of the analyzed strains were able to biosynthesize fumonisins (range 0.03-69.84 µg/g). Populations from Italy, Spain, Tunisia and Iran showed a similar percentage of fumonisin producing strains (>90%); conversely, the Egyptian population showed a lower level of producing strains (46%). Significant differences in fumonisin biosynthesis were detected among strains isolated in the same country and among strains isolated from different countries. A portion of the divergent FUM1 gene and of intergenic regions FUM6-FUM7 and FUM7-FUM8 were sequenced to evaluate strain diversity among populations. A high level of genetic uniformity inside the populations analyzed was detected. Apparently, neither geographical origin nor fumonisin production ability were correlated to the genetic diversity of the strain set. However, four strains from Egypt differed from the remaining strains. 2 of 17 semitropical and tropical regions including European [4], Mediterranean [8], African [9] and Middle Eastern [10] maize-growing areas. For example, F. verticillioides was the species isolated more frequently from maize kernels harvested in Italy [11-13], Spain [14-16], Egypt [17-21] and Iran [22]. This is also one of the species able to biosynthesize the secondary metabolites fumonisins [23]. Specifically, F. verticillioides is considered the main fumonisin producer; therefore, this is the most important species associated with fumonisin contamination of maize grains [24]. Fumonisins occur worldwide in maize, including Mediterranean [4,8,24,25] farming areas, where this is one of the most widely cultivated crops [26,27]. Fumonisin accumulation in maize grains can occur in the field, following preharvest infections, and possibly continue during grain storage [28].Contaminations strongly impair maize grain quality because of the negative impact on animal and human health [29]. Fumonisin mycotoxins can be divided into four main groups, with the most abundant fumonisins found in nature included in the B group: fumonisin B 1 (FB 1 ), fumonisin B 2 (FB 2 ) and fumonisin B 3 (FB 3 ). Among B analogues, FB 1 is the most detected fumonisin in maize as well as the most toxicologically active [24,30]. In fact, after ingestion, fumonisins may cause a wide range of toxic effects, especially towards liver and kidneys [31][32][33][34][35]. For this reason, the European Commission has established maximum limits for the sum of FB 1 and FB 2 in maize for human consumption [36,37].The amount of fumonisins found in maize kernels is a...

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confidence: 72%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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In Vitro Fumonisin Biosynthesis and Genetic Structure of Fusarium verticillioides Strains from Five Mediterranean Countries

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“…Fumonisins are the most important group of mycotoxins found in Brazilian corn and are known to be harmful to animals and to cause certain types of cancer in humans (Munkvold and Desjardins 1997;Gelderblom et al 1988;Jackson and Jablonski 2004). Although fumonisins are produced by several Fusarium species, Fusarium verticillioides is the most commonly fungi infecting corn kernels and causing ear and kernel rot (Munkvold and Desjardins 1997;Lanza et al 2014).…”

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Symptomatological aspects associated with fungal incidence and fumonisin levels in corn kernels

Lanza

Zambolim

Costa

et al. 2017

Trop. plant pathol.

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Toxigenic fungi that infect corn grains are among the major problems in Brazil as they are associated with toxicological implications for both humans and animals. Mechanical-physical methods have been used to separate contaminated grains in lots of corn kernels based on the symptoms caused by fungi. The objective of this study was to assess whether the presence or the type of symptoms were associated with fungal incidence and total fumonisin levels in corn kernel samples from the field. Kernels of two hybrids were separated visually into three groups: 1) asymptomatic or symptomatic exhibiting 2) kernel rot or 3) kernel streak symptoms. A high frequency of Fusarium spp. was observed in asymptomatic (69.5%) and kernel rot (69.9%). Stenocarpella sp. and Penicillium sp. were found in kernel rot (16.0%) and kernel streak (44.1%), respectively. High fumonisin levels (7.24 μg.g-1), above the maximum tolerated limit, were detected only in kernel rot. No association between fumonisin levels and the frequency of Fusarium spp. in kernels was found.

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Association mapping and genomic prediction for ear rot disease caused by Fusarium verticillioides in a tropical maize germplasm

et al. 2020

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Fusarium ear rot (FER), caused by Fusarium verticillioides (Sacc.) Nirenberg, is one of the major ear diseases that affect both yield and grain quality in maize (Zea mays L.), especially in tropical environments. Fusarium genetic resistance is a complex trait, controlled by several small‐effect genes and strongly influenced by the environment. We applied a comprehensive genome‐wide association study and genomic prediction for ear rot and starburst symptoms, using 291,633 high‐quality single nucleotide polymorphism (SNPs) markers in 320 tropical maize inbred lines, in two distinct locations in Brazil's southern region. Three SNPs were significantly associated with starburst symptoms, each associated with 6–8% of the phenotypic variance, and with gene models that have expression levels in ears, pericarp, and kernels, corresponding to disease infection period and suggesting some role in FER resistance. No significant SNP was associated with FER, which is an indication that the genetic architecture for this trait is highly polygenic, with potentially many variants having small effects that are not detectable in the association mapping analysis. We observed genomic prediction accuracies ranging from 0.34 to 0.4 for FER and starburst, respectively. Further research is required to validate these significant SNPs and their relationship to genes affecting FER resistance, and also to improve genomic prediction accuracies across different genetic backgrounds.

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Prevalence of fumonisin-producing Fusarium species in Brazilian corn grains

Cited by 29 publications

References 36 publications

In Vitro Fumonisin Biosynthesis and Genetic Structure of Fusarium verticillioides Strains from Five Mediterranean Countries

In Vitro Fumonisin Biosynthesis and Genetic Structure of Fusarium verticillioides Strains from Five Mediterranean Countries

Symptomatological aspects associated with fungal incidence and fumonisin levels in corn kernels

Association mapping and genomic prediction for ear rot disease caused by Fusarium verticillioides in a tropical maize germplasm

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