Production and regeneration of protoplasts from orchid Mycorrhizal Fungi Epulorhiza repens and Ceratorhiza sp.

Coelho, Irene da Silva; Queiroz, Marisa Vieira de; Costa, Maurício Dutra; Kasuya, Maria Catarina Megumi; Araújo, Elza Fernandes de

doi:10.1590/s1516-89132010000100019

Cited by 10 publications

(9 citation statements)

References 26 publications

(32 reference statements)

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“…Mycelium age is regarded as one of the main factors influencing the release of protoplasts (Coelho et al 2010 ; Wei et al 2010 ). The structure of the fungal cell wall is highly dynamic, and may vary during the cell division, growth of fungi and hyphal branching (Li et al 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…Osmotic pressure stabilizers can balance the internal and external osmotic pressure of protoplasts that have lost cell wall protection and prevent the destruction of protoplasts (Harris 1982 ). When obtaining fungal protoplasts, inorganic salts (KCl, MgSO 4 , NaCl or NH 4 Cl) or sugars (sucrose, glucose or sorbitol) are often used as osmotic stabilizers (Zhou et al 2008 ; Coelho et al 2010 ).…”

Section: Discussionmentioning

confidence: 99%

“…The use of protoplasts,—single mononuclear cells devoid of a cell wall and surrounded only by a cytoplasmic membrane, can facilitate genetic manipulation and mutagenesis of filamentous fungi and often plays a significant role in strain improvement (Roth and Chilvers 2019 ; Zhou et al 2008 ). In turn, effective protoplast production, release and mutagenesis depend on many factors including fungal species, mycelium state and protoplasting conditions (osmotic stabilizer, temperature, pH, digestion time, lytic enzyme concentration and others) whether a protoplast is mononucleate, binucleate or multinucleate (Kumari and Panda, 1992 ; Coelho et al 2010 ; Ren et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Ursodeoxycholic acid production by Gibberella zeae mutants

Kollerov

Donova

2022

AMB Expr

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Ursodeoxycholic acid (UDCA) is a highly demanded pharmaceutical steroid widely used in medicine. An ascomycete Gibberella zeae VKM F-2600 is capable of producing UDCA by 7β-hydroxylation of lithocholic acid (LCA). The present study is aimed at the improvement of the fungus productivity. The original procedures for the protoplast obtaining followed by UV mutagenesis and screening of ketoconazole-resistant mutant clones have been applied. The highest yield of G. zeae protoplasts was obtained when using the mycelium in the active growth phase, ammonium chloride as an osmotic stabilizer and treatment of the fungal cells by the lytic enzymes cocktail from Trichoderma hurzanium. The conditions for effective protoplast regeneration and the UV-mutagenesis were found to provide 6–12% survival rate of the protoplasts with superior number of possible mutations. Three of 27 ketoconazole-resistant mutant clones obtained have been selected due to their increased biocatalytic activity towards LCA. The mutant G. zeae M23 produced 26% more UDCA even at relatively high LCA concentration (4 g/L) as compared with parent fungal strain, and the conversion reached 88% (w/w). The yield of UDCA reached in this study prefers those ever reported. The results contribute to the knowledge on ascomycete mutagenesis, and are of importance for biotechnological production of value added cholic acids. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ursodeoxycholic acid production by Gibberella zeae mutants

Kollerov

Donova

2022

AMB Expr

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“…The key factors affecting protoplast release of filamentous fungi include the type of enzymes, enzyme combinations, age of mycelia, and incubation time (da Silva Coelho et al, 2010; Xiao et al, 2013). Rehman et al (2016) optimized the protoplast isolation, regeneration, and transformation efficiency of Verticillium dahlia , the causal agent of Verticillium wilt.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Polyethylene Glycol-Mediated Transformation of the Pepper Anthracnose Pathogen Colletotrichum scovillei to Develop an Applied Genomics Approach

et al. 2019

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“…During germination, the seeds cannot grow without support from a different living organism. In nature, they rely on a suitable symbiotic association with mycorrhizal fungi [20,[27][28][29][30]. For this reason, out of the large number of seeds that the orchids scatter around, perhaps one millionth of them have a chance to germinate [31].…”

Section: Introductionmentioning

confidence: 99%

Morphological Developments of Orchis purpurea Huds. Salep Orchids

Çalışkan

Kurt

Odabas

2020

Braz. arch. biol. technol.

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This article aims to monitor the development of Orchis purpurea Huds., salep orchids, of different sizes over a period of two years, and to investigate the relationship between the parameters studied. In the first step, the measurements taken at the time of planting and harvesting of tubers divided into eight different groups according to their size were subjected to variance and Duncan's test. In the second step, the relationship between the parameters was investigated by ignoring seedling groups. The relationship between the two variables was determined by correlation analysis. The significance of the relationships between planting and harvest data sets, and variable contributions were determined by canonical correlation analysis. Finally, leaf area prediction modeling was performed by applying multiple regression analysis. In variance analysis all parameters were significant. The canonical correlation between the first pair of canonical variables was 0.988 (p<0.01). The data obtained from the tubers made the greatest contribution to the explanatory power of the canonical variables. The leaf area model was formulized as LA (mm 2) =-1237.0204 + 57.7912 × LW + 16.6211 × LL where LA is leaf area, LW is leaf width, LL is leaf length and a, b, and c are coefficients.

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Production and regeneration of protoplasts from orchid Mycorrhizal Fungi Epulorhiza repens and Ceratorhiza sp.

Abstract: ABSTRACT

Cited by 10 publications

References 26 publications

Ursodeoxycholic acid production by Gibberella zeae mutants

Ursodeoxycholic acid production by Gibberella zeae mutants

Optimization of Polyethylene Glycol-Mediated Transformation of the Pepper Anthracnose Pathogen Colletotrichum scovillei to Develop an Applied Genomics Approach

Morphological Developments of Orchis purpurea Huds. Salep Orchids

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