Optimization of Polyethylene Glycol-Mediated Transformation of the Pepper Anthracnose Pathogen Colletotrichum scovillei to Develop an Applied Genomics Approach

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

“…Here, the regeneration capacity depends on the biomolecule delivery method employed and whether a stable transformation is desired (i.e., constitutive or transient; Cunningham et al, 2018). Various biological, chemical, and physical methods are available to deliver genetic materials into plant cells, including the aforementioned AMT, viral-mediated transformation, polymer-mediated delivery (e.g., polyethylene glycol [PEG)]), particle bombardment (gene gun-mediated or biolistic transformation), and electroporation (Ahmed et al, 2018;Mohammed et al, 2019;Shin et al, 2019;Tian et al, 2019;Imai et al, 2020;Ozyigit, 2020). The features of the conventional transformation methods are summarized in Table 1.…”

Homeobox Transcription Factors Are Required for Fungal Development and the Suppression of Host Defense Mechanisms in the Colletotrichum scovillei -Pepper Pathosystem

“…Based on the socioeconomic impact and difficulty involved in disease management of fruit anthracnose in a wide range of crops, we previously elucidate the whole genome of C . scovillei to better understand the molecular mechanisms involved in fruit anthracnose, which have been relatively uncharacterized at the molecular level ( 8 , 31 , 32 ).…”

Antagonistic and Plant Growth-Promoting Effects of Bacillus velezensis BS1 Isolated from Rhizosphere Soil in a Pepper Field

“…C. acutatum , C. coccodes , C. dematium , and C. gloeosporioides are known to cause pepper anthracnose in Korea, and C. acutatum is considered to be the major pathogen of the disease ( Kim et al, 2008 ). However, C. scovillei , a member of the C. acutatum species complex, has recently been identified as the dominant pathogen causing pepper anthracnose in Korea ( Oo et al, 2017 ; Shin et al, 2019 ). As it is a newly identified species in Korea, control strategies for this pathogen are limited.…”

“…This revealed that BS1-mediated growth of lettuce seedlings was not significant (data not shown), which suggests that BS1 may not be associated with modulation of auxins. Because C. scovillei has only recently been identified as a Colletotrichum species causing anthracnose on pepper in Korea ( Oo et al, 2017 ; Shin et al, 2019 ), there are few studies on biological or chemical controls against the pathogen. Our results provide basic information on biological control of C. scovillei causing pepper anthracnose.…”

“…Drops (20 μl) of BS1 suspension were added to the paper disks and incubated in the dark at 25°C for 4 days (B. cinerea, R. solani, and S. sclerotiorum) or 7 days (C. scovillei and C. gloeosporioides). (Shin et al, 2019), C. gloeosporioides (Han et al, 2015), B. cinerea (Kim et al, 2016), R. solani (KACC 40108), and S. sclerotiorum (KACC 40457) grown on potato dextrose agar (PDA; MBcell, Seoul, Korea) were transferred to the center of fresh PDA plates, and paper disks were placed at the edge of the PDA plates. Isolated bacterial strains were incubated in LB broth overnight (16 h) at 25°C with shaking at 150 rpm, and 10 μl of bacterial culture was dropped on the paper disks.…”