Glucose administration inhibits the hepatic activation of gluconeogenesis promoted by insulin-induced hypoglycemia

Abstract

The activation of hepatic gluconeogenesis in male Wistar adult 6 h fasted rats during insulin-induced hypoglycemia (IIH) was previously demonstrated. In this study, the effects of intraperitoneal (ip) glucose (100 mg/kg) on the activation of liver gluconeogenesis during IIH was investigated. Thus, 6 h fasted rats that received ip regular insulin (1 U/kg) and 30 min later ip saline (Control group) or glucose (Experimental group) were compared. All the experiments were executed 60 min after insulin injection. The glycemia of Control and Experimental groups were not different (P > 0.05). To investigate gluconeogenesis, liver perfusion experiments were performed. The results demonstrated that excepting glycerol, livers from rats which received ip glucose showed lower (Therefore, the absence of glucose recovery after the glucose administration was mediated, at least in part, by an inhibition of hepatic gluconeogenesis.

Antidiabetic activities of ethanol extract of dry matters of culture broth of Coriolus versiolor in submerged culture

“…control the blood glucose level only when they are regularly administered, but these treatments are tedious and have several disadvantages (such as hypoglycemia, obesity, etc.) (Bhatnagar, 1998;May et al, 2002;Galende et al, 2009). The management of hyperglycemia or hyperlipidemia with minimal side effects in clinical experience and relatively low costs is still a challenge to the medical system.…”

Infliximab treatment prevents hyperglycemia and the intensification of hepatic gluconeogenesis in an animal model of high fat diet-induced liver glucose overproduction

“…Since in situ perfused livers reflected the in vivo conditions of the animal immediately before the liver isolation (Galende et al 2009;OliveiraYamashita et al, 2009) and considering that mice treated with a HFD were a suitable experimental model to induce the liver glucose overproduction (Tomas et al 2010;Wu et al 2010), this experimental approach was used. As showed by Table 1, the treatment with infliximab prevented the glucose overproduction during the infusion of L-alanine in the liver from HFD mice (p<0.05: HFD + Infliximab vs. HFD + Saline).…”

“…After laparotomy, blood was collected from the cava vein for glucose determination (Bergmeyer and Bernt 1974) and the liver perfusion was started immediately. The in situ liver perfusion technique was done as previously described (Galende et al 2009;Oliveira-Yamashita et al 2009). Figure 1 showed a demonstrative experiment in which after a preinfusion period (10 min), L-alanine was infused during 60 min, followed by a post-infusion period (10 min without L-alanine) to allow the return of basal glucose production values of the pre-infusion period.…”

Investigation of the Acute Effects of Dry Extract of Glycine Max on Postprandial Glycemia in Rats

“…Considering that in Brazil there is not another antidiabetic drug with ability to slow intestinal absorption, the possibility of using new compounds instead acarbose has been investigated. In fact there are many in vitro studies showing inhibition of α-glucosidase by isoflavones from plants [20][21][22] . However, there is absence of in vivo studies demonstrating antihyperglycemic properties of these compounds.…”