Morphological markers for microspore developmental stage in maize

Moraes, Ana Paula de; Bered, Fernanda; Carvalho, Fernando Irajâ Félix de; Kaltchuk-Santos, Eliane

doi:10.1590/s1516-89132008000500006

Cited by 5 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is consistent with the previous cytological study results, i.e., the premature microspore abortion and tapetum degradation at MS stage is one of the prominent characteristics of CMS-C in maize ( Chen & Duan, 1988 ). Moraes, Bered & Kaltchuk-Santos (2008) suggested spikelet lengths are reliable parameters for predicting the specific microspore developmental stage of maize under the same planting condition. As shown in Fig.…”

Section: Resultsmentioning

confidence: 96%

Comparative transcriptome analysis of isonuclear-alloplasmic lines unmask key transcription factor genes and metabolic pathways involved in sterility of maize CMS-C

Zhao

Liu

et al. 2017

PeerJ

View full text Add to dashboard Cite

Although C-type cytoplasmic male sterility (CMS-C) is one of the most attractive tools for maize hybrid seed production, the detailed regulation network of the male sterility remains unclear. In order to identify the CMS-C sterility associated genes and/or pathways, the comparison of the transcriptomes between the CMS-C line C48-2 and its isonuclear-alloplasmic maintainer line N48-2 at pollen mother cell stage (PS), an early development stage of microspore, and mononuclear stage (MS), an abortive stage of microspore, were analyzed. 2,069 differentially expressed genes (DEGs) between the two stages were detected and thought to be essential for the spikelet development of N48-2. 453 of the 2,069 DEGs were differentially expressed at MS stage between the two lines and thought to be participated in the process or the causes of microspore abortion. Among the 453 DEGs, 385 (84.99%) genes were down-regulated and only 68 (15.01%) genes were up-regulated in C48-2 at MS stage. The dramatic decreased expression of the four DEGs encoding MYB transcription factors and the DEGs involved in “polyamine metabolic process”, “Cutin, suberine and wax biosynthesis”, “Fatty acid elongation”, “Biosynthesis of unsaturated fatty acids” and “Proline metabolism” might play an important role in the sterility of C48-2. This study will point out some directions for detailed molecular analysis and better understanding of sterility of CMS-C in maize.

show abstract

Section: Resultsmentioning

confidence: 96%

Comparative transcriptome analysis of isonuclear-alloplasmic lines unmask key transcription factor genes and metabolic pathways involved in sterility of maize CMS-C

Zhao

Liu

et al. 2017

PeerJ

View full text Add to dashboard Cite

show abstract

“…According to the literature data, the most suitable for androgenesis induction in crop plants are microspores in the late phase or early-bicellular pollen grain (Kiszczak et al 2005, Dubas et al 2010, Soriano et al, 2013. Many authors indicate, that the bud length is a good morphological indicator of microspore development phases and thus it can be considered as a valid marker of anther usability for anther cultures (Moraes et al, 2008, Parra -Vega et al, 2013. Julião et al (2015) with the use of flow cytometer confirmed a correlation between the microspre development phases in tomato anthers and the length of the bud, in which they were located.…”

Section: Discussionmentioning

confidence: 99%

Research on in vitro haploidization of heterozygotic breeding lines of tomato (Solanum lycopersicum L.)

Kiszczak¹,

Kowalska²,

Burian³

2021

Biul. IHAR

View full text Add to dashboard Cite

Experiments were made in order to examin the influence of various factors on the induction of androgenesis in heterozygous breeding material of tomato. The factors like: length of buds the manner of sterilization, type and the composition of induction media, genotype and thermal shock were included in conducted experiments. Most of all sterile cultures with the highest number of anther-derived callus were obtain by applying 2,5% calcium hypochlorite for 5 minutes. Anther-derived calli was obtained in 9 breeding lines from 18 used genotypes. The induction medium with the composition based on B5 medium with the addition of 750 mg L-1 calcium chloride and 100 g L-1 sucrose proved to be the best for inducing androgenesis. The addition of thidiazuron and NAA to this medium in the following season and silver nitrate in the other experiminet improved the efficiency of this process, which was depended on the genotype. Despite the lack of statistically significant differences, the highest number of anther-derived calli was obtained when anthers were cooled for 2 days in +4oC in the refrigerating chamber.

show abstract

“…Bandera. In the study of morphological markers for the development of microspores in maize, Moraes et al (2008) stated that anther length was the most reflected cytological stage of microsporogenesis. In wheat, it is also found that the length of the anther is considered an appropriate morphological marker for the assessment of the specific development of microspores (Immonean and Antila, 1998).…”

Section: Developmentalmentioning

confidence: 99%

Induction of Microspore Embryogenesis of Eggplant (<i>Solanum melongena</i> L.) ‘Gelatik’

Pagalla

Indrianto

Maryani

et al. 2020

J.Tropical Biodiversity Biotechnology

View full text Add to dashboard Cite

The haploid or double haploid plant of eggplants could be produced from microspore culture (embryogenesis of microspores). In the breeding programs, microspore can be developed into an embryo directly after exposure to stress treatment during cultured. Stress (temperature and starvation medium) is an important factor in the induction of embryogenesis microspore. This study aims to induced embryogenic microspores from eggplant CV. Gelatik. The stage late-uninucleate microspore (Vacuolate Microspore/VM) and early binucleate (Young Bicellular Pollen/YBP) are the suitable stages to induce multinucleate structure. There are 3 methods used in this research; 1) Determination of the stage development of microspore based on flower buds length and anther length. 2) Induction of embryogenic microspore on the pre-treatment and starvation medium. 3) After giving pre-treatment for 4 days, micropores were transferred to culture medium A2 at 28oC in dark conditions to induce the multicellular structures. This study reported that 50-68.51% of the VM+YBP stage obtained in the range of flower bud lengths of 10-17 mm, and 5.0-6.9 mm, the range of anther length containing VM+YBP of 50-77.48%. The pre-treatment heat shock at 33oC in the medium B for 2 days, produced embryogenic microspores with a high percentage, that is about 50.19%, while microspores at 25oC and 4oC respectively 46.17% and 49.28%. Pre-treatment for 4 days at 4 oC, 25 oC, and 33oC with the percentage of embryogenic microspores apiece 32.87%, 27.45%, and 37.34%. The multicellular (starlike) structure begins forming on the fifth day of incubation in culture medium (A2) after pre-treatment in B medium at 33oC.

show abstract

Morphological markers for microspore developmental stage in maize

Abstract: ABSTRACT

Cited by 5 publications

References 12 publications

Comparative transcriptome analysis of isonuclear-alloplasmic lines unmask key transcription factor genes and metabolic pathways involved in sterility of maize CMS-C

Comparative transcriptome analysis of isonuclear-alloplasmic lines unmask key transcription factor genes and metabolic pathways involved in sterility of maize CMS-C

Research on in vitro haploidization of heterozygotic breeding lines of tomato (Solanum lycopersicum L.)

Induction of Microspore Embryogenesis of Eggplant (<i>Solanum melongena</i> L.) ‘Gelatik’

Contact Info

Product

Resources

About