Biodegradation of diesel oil by yeasts isolated from the vicinity of suape port in the state of Pernambuco - Brazil

Miranda, Rita de Cássia Mendonça de; Souza, Cynthia Silva; Gomes, Edelvio de Barros; Lovaglio, Roberta Barros; Lopes, Carlos Edison; Sousa, Maria de Fátima Vieira de Queiroz

doi:10.1590/s1516-89132007000100018

Cited by 41 publications

(17 citation statements)

References 12 publications

(8 reference statements)

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“…Los aislamientos C1C y C3RCS corresponden a la especie R. calyptogenae, (99.8 % de identidad) y el aislamiento C1NR, corresponde a R. dairenensis (99.8% de identidad). Otras especies de este género como R. mucilaginosa (Goulart et al, 2014) y R. aurantiaca (Miranda et al, 2007), poseen la capacidad de degradar queroseno, gasolina y aceite diésel, así como algunos hidrocarburos alifáticos como decano, nonano y dodecano. En el presente estudio, los aislamientos crecieron en un medio con gasolina, lo cual indicaría su adaptación a habitar tanques en donde constantemente están en contacto con dicho combustible.…”

Section: Discussionunclassified

Aislamiento e identificación de levaduras degradadoras de hidrocarburos aromáticos, presentes en tanques de gasolina de vehículos urbanos

Delgadillo-Ordoñez

Posada-Suárez

Marcelo

et al. 2017

Rev. colomb. biotecnol.

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Aislamiento e identificación de levaduras degradadoras de hidrocarburos aromáticos, presentes en tanques de gasolina de vehículos urbanosIsolation and identification of aromatic hydrocarbon degrading yeasts present in gasoline tanks of urbans vehicles DOI: 10.15446/rev.colomb.biote.v19n2.70278 RESUMENSe obtuvieron aislamientos de levaduras a partir de muestreos en tanques de combustible de vehículos urbanos, con el objeto de evaluar su potencial actividad de degradación de hidrocarburos aromáticos derivados del petróleo. Se realizaron ensayos de crecimiento en medio mínimo mineral sólido utilizando distintos hidrocarburos (benceno, tolueno, naftaleno, fenantreno, y pireno). Los aislamientos que presentaron crecimiento notorio en alguno de los hidrocarburos aromáticos policíclicos fueron identificados mediante secuenciación Sanger de los marcadores moleculares ITS1 e ITS2 del ARNr. Se obtuvieron 16 aislados de levaduras, de las cuales tres presentaron crecimiento conspicuo con hidrocarburos aromáti-cos como única fuente de carbono. Las cepas identificadas pertenecen al género Rhodotorula y corresponden a las especies Rhodotorula calyptogenae (99,8% de identidad) y Rhodotorula dairenensis (99,8% de identidad). Dichas cepas presentaron crecimiento en benceno, tolueno, naftaleno, fenantreno. En este estudio se reporta por primera vez la presencia de levaduras del género Rhodotorula que habitan ABSTRACTYeast isolates were obtained from fuel tanks of vehicles in order to assess their potential use in the degradation of aromatic hydrocarbons. Growth assays were performed in minimum mineral medium using different aromatic hydrocarbons (benzene, toluene, naphthalene, phenanthrene, and pyrene) as the sole carbon source. Isolates that showed growth in any of the tested polycyclic aromatic hydrocarbons were identified by Sanger sequencing of the ITS1 and ITS2 rDNA molecular markers. A total of 16 yeasts strains were isolated, and three showed remarkable growth in media with aromatic hydrocarbons as the sole carbon source. These strains belong to the genus Rhodotorula, and correspond to the species Rhodotorula calyptogenae (99,8% identity) and Rhodotorula dairenensis (99,8% identity). These strains grew in benzene, toluene, naphthalene, phenanthrene and pyrene. This study demonstrates for the first time that yeasts of the genus Rhodotorula inhabit pipelines and fuel tanks of vehicles and that remove aromatic hydrocarbons that are environmental pollutants. Our results suggest that these yeasts are potential candidates for aromatic hydrocarbon degradation as part of bioremediation strategies.

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Section: Discussionunclassified

Aislamiento e identificación de levaduras degradadoras de hidrocarburos aromáticos, presentes en tanques de gasolina de vehículos urbanos

Delgadillo-Ordoñez

Posada-Suárez

Marcelo

et al. 2017

Rev. colomb. biotecnol.

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“…However, one should take into account that all of the isolates have a potential to degrade hydrocarbons because they were isolated from a medium containing oil as the sole carbon source, although their activity may have been less accelerated at the onset of biological oxidation compared with the selected isolates [11] [14] [21] [22].…”

Section: Selection No 2: Oxidation Test With Acclimatizationmentioning

confidence: 99%

“…This technique follows the principle, and the indicator is the verification of the occurrence of biological oxidation of hydrocarbons, in which the DCPIP in the culture medium acts as the electron acceptor in the oxidation process [14].…”

mentioning

confidence: 99%

Isolation and Selection of Fungi for Degrading Saturated Hydrocarbons, Aromatic Hydrocarbons and NSO Compounds

Lima¹,

Oliveira²,

Geris³

et al. 2017

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Petroleum hydrocarbons are recalcitrant contaminants that have various impacts on the natural ecosystem. Microorganisms have been widely used in recent years to remove the hydrocarbons that are present in crude oil. The objective of this research was to isolate and select microorganisms that have the potential to degrade hydrocarbons present in mangrove sediments. In present study, we have isolated fungi from clean sediment contaminated with different fractions (i.e., saturated hydrocarbons, aromatic, and no hydrocarbon compounds (NSO)) of two types of oil. There were two types of oxidation tests were performed: one in multi well plates and the other in stirring and temperature control. Screening tests were conducted to detect the biodegradation of petroleum fractions with 72 fungal isolates over a period between 12 and 48 hours. With the two oxidation tests, the 2,6-dichlorophenol-indo phenol (DCPIP) indicator made it possible to select fungi with the potential to degrade the three main fractions of oil in the Reconcavo and Campos Basins. It was observed that the fungal isolates in the cleaned sediment were able to oxidize the three fractions of both types of oil. It was also concluded that some isolated strains oxidize oil faster and more efficiently than others. The formation of a consortium with the isolated consortium was a potential for the increase of the degradation of oil in the environment.

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“…Sood and Lal ( 2009 ) isolated a Candida digboiensis from soil samples contaminated with acidic oily sludge (pH 1-3) which could degrade 73% of the total petroleum hydrocarbons present in the medium at pH 3 in a week. Miranda et al ( 2007 ) investigated the potential of the diesel degrading yeasts, Rhodotorula aurantiaca and Candida ernobii. They reported complete degradation of tetradecane, 5 methyl-octane and octadecane by C. ernobii and 93% for decane, 38.4% for nonane and 22.9% for dodecane with R. aurantiaca .…”

Section: Bioaugmentationmentioning

confidence: 99%

Bioremediation of Petroleum Hydrocarbons in Soils

Kulkarni

Palande

Deshpande

2011

Microorganisms in Environmental Management

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Crude oil is a complex mixture of hydrocarbons, basically composed of aliphatic, aromatic and asphaltene fractions along with nitrogen, sulfur and oxygen containing compounds. Major causes of oil contaminated soils include: leakage of storage tanks and pipelines, land disposal of petroleum wastes and accidental spills. Apart from damaging crops and affecting the fertility of soil, crude oil on the fi elds also affects livestock. The loss of soil fertility and toxicity of petroleum hydrocarbon at higher concentrations have been linked to displacement of nutrients and nutrient linkage, reduction in phosphorus and nitrogen availability, and anoxic conditions. Moreover, it can cause microbial community structure changes and a decrease in microbial diversity. The aromatics in crude oils such as a -pinene, limonene, camphene, and isobornyl acetate were observed to be inhibitory to the microorganisms. In recent years, there has been increasing interest in developing on site and in situ techniques for remediation of oil-contaminated soils. Bioremediation can be achieved by natural attenuation, biopiling, bioaugmentation, phytoremediation or rhizoremediation, singly or in combination. Numerous genera of bacteria are known as good hydrocarbon degraders. Most of them belong to Aeromonas, Alcaligenes, Acinetobacter, Arthobacter, Bacillus, Brevibacterium, Mycobacterium, Pseudomonas, Rhodococcus, Sphingomonas,

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Biodegradation of diesel oil by yeasts isolated from the vicinity of suape port in the state of Pernambuco - Brazil

Abstract: ABSTRACT

Cited by 41 publications

References 12 publications

Aislamiento e identificación de levaduras degradadoras de hidrocarburos aromáticos, presentes en tanques de gasolina de vehículos urbanos

Aislamiento e identificación de levaduras degradadoras de hidrocarburos aromáticos, presentes en tanques de gasolina de vehículos urbanos

Isolation and Selection of Fungi for Degrading Saturated Hydrocarbons, Aromatic Hydrocarbons and NSO Compounds

Bioremediation of Petroleum Hydrocarbons in Soils

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