Effect of plant growth regulators in the rooting of Pinus cuttings

Henrique, Andréia; Campinhos, Eduardo Nogueira; Ono, Elizabeth Orika; Pinho, Sheila Zambello de

doi:10.1590/s1516-89132006000300002

Cited by 56 publications

(37 citation statements)

References 15 publications

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“…However, the value of correlation coefficient for the relationship between the percentage of shoots that actually formed root and increasing concentration of IBA was R = 0.871, suggesting a negative relationship between the application of increasing concentrations of IBA and rooting, at least up to certain level (Figure 2). The relationship observed between auxin level and rooting potential has been recorded for other plants where high levels of auxin may have inhibitory effect over the rooting process (Pedroti and Voltolini, 2001;Erig and Schuch, 2004;Henrique et al, 2006;Maia and Botelho, 2008). Following this, the regenerated plants were acclimatized and transferred to the green house ( Figure 1F).…”

Section: Rootingsupporting

confidence: 55%

Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior

Nogueira¹,

Soares²,

Ibrahim³

et al. 2011

Afr. J. Biotechnol.

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Using cut pieces cotyledons from germinating zygotic embryos of Jatropha curcas, we monitored the series of anatomical events leading to the generation of shoot through organogenesis by histological analysis. 14 days old cotyledons that were pre-cultured in a half strength Murashige and Skoog (MS) media supplemented with 100 mg/L myoinositol and 10 mg/L thiamine HCl, were cultured in organogenic competence induction media (CIM) comprising of MS salts with 1.5 mg/L benzyl adenine (BA) and 0.05 mg/L indole-3-butyric acid (IBA) and incubated for the induction of organogenic competence. Following their sequential transfer to shoot induction media (containing MS + 1.5 mg/L BA, 0.05 mg/L IBA and 0.5 mg/L GA 3 ); shoot elongation media (containing MS + 0.3 mg/L BA) and rooting media (containing half strength MS + IBA at different concentrations), we selected individual explants and subjected them to histological analysis in order to study the morphological changes occurring during organogenesis. Our findings show that to induce organogenesis using cut pieces of cotyledons, these tissues must be harvested at least by the 14th day following germination. Optimal organogenic competence was attained after 21 days incubation period in the dark where most of the explants showed evidence of protruding shoots surrounded by calli with various morphological features. Up to 53.91% of the total explants cultured in this study produced well defined shoots with each explant producing an average number of 1.5 shoots bearing two to eight leaves. We recorded the highest percentage of root formation, which stood at 27.50% when the shoots were cultured in a rooting media containing 0.3 mg/L IBA. Histological analysis of the different events occurring during the process of organogenesis suggest that the protuberances arising from parenchymatous cells and perhaps bundle sheath forming meristematic centres acquiring organogenic competence have a multicellular origin, indicating that the regeneration process takes place through direct organogenesis.

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Section: Rootingsupporting

confidence: 55%

Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior

Nogueira¹,

Soares²,

Ibrahim³

et al. 2011

Afr. J. Biotechnol.

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“…However, such finding is in accordance with Henrique et al (2006), reported that Pbz at 100 mg/l with IBA or NAA increased total phenols of Pinus caribaea var hondurensis.…”

Section: Phenols Content In the Leavessupporting

confidence: 92%

Effect of Pinching and Paclobutrazol (Pbz) on Vegetative Growth of Russelia equisetiformis for Using as a Pot Plant

2015

NIDOC-ASRT

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HE PRESENT experiment was performed at the nursery of Horticulture Research Institute, Agriculture Research centre, Giza, Egypt during two successive seasons 2013 and 2014. It intended to dwarf vegetative growth of Russelia equisetiformis for using as a pot plant. Thus, the individual and the combined effects of different pinching treatments (after either one or two months from planting) and various levels of paclobutrazol (0, 100, 150 and 200 mg/l) were applied as a foliar spray, commencing from one month of planting and then at weekly intervals till the experiment termination in (September15 th ) in both seasons. The results emphasized that using pinching treatment for only one time produced the highest number of secondary shoots/plant and increased fresh and dry weight of shoots/plant as well as flower number/plant. Meanwhile, treating plants with pinching treatment for two times proved its superiority in reducing plant height with increasing number of main shoots, root length and number of roots/plant. Additionally, negligible and insignificant differences were observed for fresh and dry weights of roots due to using pinching treatments in both seasons.Plant height was gradually decreased by increasing paclobutrazol levels in both seasons, whereas number of roots/plant was progressively increased by increasing paclobutrazol level in the same seasons. Applying paclobutrazol at 100 mg/l revealed a clear increment in either fresh or dry weight of shoots/plant. Moreover, root length was increased due to using the same paclobutrazol level (100 mg/l). Supplying plants with paclobutrazol at 150 mg/l proved its mastery in raising number of main and second shoots/plant. Also, the same treatment proved its superiority for raising fresh and dry weight of roots. Meanwhile, paclobutrazol treatment at 200 mg/g gave rise to the utmost high values of flower number/plant.Referring to chemical constituents of vegetative growth parts, pinching treatment for two times proved its mastery in raising chlorophyll (a) and carotenoids contents, whereas it slightly increased chlorophyll (b). Meanwhile, the same treatment caused a slight increment in phenols content, but decreased indoles content in the T

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“…These auxins may not have direct effect on the development of shoots, but may be effective mostly through induction of roots (Husen and Pal, 2007). They promote rooting in the plants through changes in the biochemical systems of the plants (Henrique et al, 2006). Among various auxins, IBA is known to stimulate rooting more efficiently due to its weak toxicity and greater stability for induction of roots (Han et al, 2009).…”

Section: Rooting Percentage (%)mentioning

confidence: 99%