In vitro propagation of orchid (Dendrobium nobile) var. Emma white

Sana, Asghar; Ahmad, Touqeer; Ahmad, Irshad; Yaseen, Mehwish

doi:10.5897/ajb10.401

Cited by 44 publications

(7 citation statements)

References 17 publications

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“…In addition, the application of cytokinins have been able to increase the number of shoots in orcid (Dendrobium nobile ISSN: 2414-4592 DOI: 10.20474/jater-2.4.4 TAF Publishing var. Emma white) which represents the importance of the exogenous cytokinin to promote the multiple shoots formation [27]. Similar to this study, the used of Kn has been able to produced plantlets of Oryza sativa L. Var.…”

Section: E Fresh and Dry Weightsupporting

confidence: 71%

Effects of different plant growth hormones on in vitro regeneration of a new Malaysian rice variety (Oryza Sativa L. Var. Mria 1) from stem explants

2016

J. adv. ethnol. eng. res.

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Section: E Fresh and Dry Weightsupporting

confidence: 71%

Effects of different plant growth hormones on in vitro regeneration of a new Malaysian rice variety (Oryza Sativa L. Var. Mria 1) from stem explants

2016

J. adv. ethnol. eng. res.

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“…The highest number of roots per explant was found in the case of P. insigne cultivated in the presence of 2.5 mg•dm −3 IAA (3.3) in comparison to those grown on the medium supplemented with 5 mg•dm −3 NAA (2.2). A low number of roots after supplementation of the medium with NAA was also noted by Asghar et al [8] in Dendrobium nobile 'Emma White'. Additionally, the authors stated that the increasing concentration of the auxin seedlings produced fewer roots.…”

Section: The Paphiopedilum Insigne Rooting System In Vitro Depending supporting

confidence: 69%

“…Positive effects of IAA in a low concentration of 0.05 mg·dm −3 on the presence of roots was confirmed in the case of Geoderum purpureum [ 14 ], and in a concentration of 1 mg·dm −3 in Dendrobium transparent [ 33 ]. The reports of Asghar et al [ 8 ] proved the positive influence of IBA on the tested features. The authors demonstrated the desirability of the use of IBA in concentrations of 2 mg·dm −3 in Dendrobium nobile ‘Emma White’.…”

Section: Resultsmentioning

confidence: 94%

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Paphiopedilum insigne Morphological and Physiological Features during In Vitro Rooting and Ex Vitro Acclimatization Depending on the Types of Auxin and Substrate

Poniewozik

Parzymies

Szot

et al. 2021

Plants

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To obtain healthy and good quality plants from in vitro cultivation, it is necessary to produce plantlets with well-developed rooting systems because they must undergo acclimatization, a final and a very difficult stage of micropropagation. In the present research, the effect of auxins NAA, IAA and IBA in concentrations of 0.5; 1; 2.5 and 5 mg·dm−3 on the Paphiopediluminsigne in vitro rooting was studied, and it was noted that 1 mg·dm−3 of IAA or IBA enabled the obtaining of a lot of rooted and good quality plantlets. The subsequent influence of the two most advantageous auxins on the acclimatization of plantlets in different substrates (sphagnum moss, sphagnum moss + substrate for orchids, substrate for orchids, substrate for orchids + acid peat) was tested, in the means of morphological features of plants and their physiological parameters, i.e., chlorophyll fluorescence (FV, Fm, Fv/Fm), stress enzyme activity (catalase, ascorbate peroxidase), and water balance. Considering all the tested features, it might be stated that the best results were obtained when explants were rooted in vitro in the presence of 1 mg·dm−3 of IAA and then planted ex vitro in substrate for orchids.

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“…However, discrete decreases were observed at concentrations above 0.5 mg L -1 BA for shoot dry matter. Contrasting results were observed by Asghar, Ahmad, Hafiz, and Yaseen (2011), who verified that BA concentrations up to 2.0 mg L -1 BA promoted significant increases in shoot dry matter of Dendrobium nobile var. Emma White.…”

Section: Effects Of Growth Regulators On Seedling Multiplication and ...mentioning

confidence: 65%

Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments

Viana

Cerqueira

Alves

et al. 2022

Acta Sci. Biol. Sci.

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Oeceoclades maculata is a terrestrial orchid species that has potential for commercial purposes. Taking that into consideration, the present investigation aimed at studying its germination and initial development in vitro as well as its acclimatization. The influence of Murashige and Skoog (MS), Knudson C (KC), and Vacin and Went (VW) media in the presence and absence of 0.3% activated charcoal on in vitro germination and protocorm development were investigated. The effects of different concentrations of BA in combination with 0.5 mg L-1 NAA on seedling multiplication and growth were evaluated. The possibility of using dark-grown stem segments for micropropagation and acclimatization under laboratory and field conditions was also assessed. The results indicated that the most adequate media for germination were full-strength MS enriched with activated charcoal or KC supplemented with 1.5 mg L-1 BA in combination with 0.5 mg L-1 NAA. In terms of protocorm development, KC supplemented with 1.5 mg L-1 BA alone or in combination with 0.5 mg L-1 NAA provided the best results. The addition of 1.5 mg L-1 BA in combination with 0.5 mg L-1 NAA to KC medium favored the best results for seedling multiplication and development. The use of dark-grown stem segments is a viable alternative for the micropropagation of O. maculata. Regarding acclimatization, 100% survival of plants was observed during the initial phases and under field conditions average survival was 53.33%.

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In vitro propagation of orchid (Dendrobium nobile) var. Emma white

Abstract: Axillary buds of orchid

Cited by 44 publications

References 17 publications

Effects of different plant growth hormones on in vitro regeneration of a new Malaysian rice variety (Oryza Sativa L. Var. Mria 1) from stem explants

Effects of different plant growth hormones on in vitro regeneration of a new Malaysian rice variety (Oryza Sativa L. Var. Mria 1) from stem explants

Paphiopedilum insigne Morphological and Physiological Features during In Vitro Rooting and Ex Vitro Acclimatization Depending on the Types of Auxin and Substrate

Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments

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