Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study

Pujatti, Priscilla Brunelli; Simal, Carlos J. R.; Santos, Raquel Gouvêa dos

doi:10.1590/s1516-89132005000700002

Cited by 6 publications

(3 citation statements)

References 10 publications

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“…The toxic purified fractions of F3.1.6 and F3.1.9 were labeled with 99m Tc using stannous chloride and sodium borohydride as reducing agents (14)(15)(16). Preliminary studies were performed to establish the optimum conditions for obtaining the highest yield of labeled venom.…”

Section: Labeling With 99m Tcmentioning

confidence: 99%

Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom

Shirmardi¹,

Shamsaei²,

Ghanei³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:Iranian scorpions belong mainly to the Buthidae and Scorpionidae families, distributed into 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming; amongst which Mesobuthus eupeus is the most common. Its venom contains several toxin fractions that may affect the ion channel. In the present study purification, labeling and biological evaluation of M. eupeus venom are described. For separation, soluble venom was loaded on a chromatography column packed with Sephadex G-50 gel. Subsequently, the fractions were collected according to UV absorption at a wavelength of 280 nm. Toxic fraction (F3) was loaded on an anionic ion exchanger resin and then on a cationic resin. Finally, toxic subfractions F3.1.6 and F3.1.9 were labeled with 99m Tc and injected into normal mice to distinguish excretion pathway. The venom toxic fraction was successfully obtained in its purified form. Radiolabeling of toxic fractions was performed at high specific activity with radiochemical purity of more than 97 and 95% respectively for F3.1.6 and F3.1.9. Biodistribution studies in normal mice with two toxic fractions usually show rapid clearance of the compounds from blood and tissue except for kidneys. Since tissue distribution studies are very important for clinical purpose, the present findings suggest that 99m Tc labeling of venom is a useful tool for in vivo studies and comprises an excellent approach to monitoring the process of biodistribution and kinetics of toxins.

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Section: Labeling With 99m Tcmentioning

confidence: 99%

Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom

Shirmardi¹,

Shamsaei²,

Ghanei³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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“…In past decades, PK studies of animal toxins radiolabeled with Iodine-125 ( 125 I) or Technetium-99m ( 99m Tc) have yielded important findings in the toxinology field [ 13 , 14 , 15 ]. For our purpose, the physical properties of those radionuclides imposed some limitations on the performance of studies with imaging techniques or PK studies longer than 24 h. For instance, 125 I has a long half-life (59.4 days) but its gamma energy (35.4 keV) does not work with common SPECT (Single Photon Emission Computed Tomography), while 99m Tc possesses excellent gamma energy (140 keV) but has a short half-life (6 h).…”

Section: Discussionmentioning

confidence: 99%

Biodistribution and Lymphatic Tracking of the Main Neurotoxin of Micrurus fulvius Venom by Molecular Imaging

Vergara

Castillo

Romero-Piña

et al. 2016

Toxins

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The venom of the Eastern coral snake Micrurus fulvius can cause respiratory paralysis in the bitten patient, which is attributable to β-neurotoxins (β-NTx). The aim of this work was to study the biodistribution and lymphatic tracking by molecular imaging of the main β-NTx of M. fulvius venom. β-NTx was bioconjugated with the chelator diethylenetriaminepenta-acetic acid (DTPA) and radiolabeled with the radionuclide Gallium-67. Radiolabeling efficiency was 60%–78%; radiochemical purity ≥92%; and stability at 48 h ≥ 85%. The median lethal dose (LD50) and PLA2 activity of bioconjugated β-NTx decreased 3 and 2.5 times, respectively, in comparison with native β-NTx. The immune recognition by polyclonal antibodies decreased 10 times. Biodistribution of β-NTx-DTPA-67Ga in rats showed increased uptake in popliteal, lumbar nodes and kidneys that was not observed with 67Ga-free. Accumulation in organs at 24 h was less than 1%, except for kidneys, where the average was 3.7%. The inoculation site works as a depot, since 10% of the initial dose of β-NTx-DTPA-67Ga remains there for up to 48 h. This work clearly demonstrates the lymphatic system participation in the biodistribution of β-NTx-DTPA-67Ga. Our approach could be applied to analyze the role of the lymphatic system in snakebite for a better understanding of envenoming.

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“…In this study, direct labeling method was chosen, and for this, stannous chloride was used as the reducing agents (Pauwels, 1993 andPujatti et al, 2005). Preliminary researches by authors were carried out to establish the optimum conditions to obtain the highest yield from labeling the mentioned fraction.…”

Section: Labeling and Radiochemical Analysis Of Toxic Fractionmentioning

confidence: 99%

Radiolabeling of Iranian Androctonus crassicauda Scorpion Venom with Technetium-99m for Biological Studies

Yekany¹,

Mofrad²,

Shirmardi³

et al. 2021

Preprint

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Background: Androctonus crassicauda has a neurotoxin venom that can affect most of the vital organs of the body and result in death. In this study 99mTc-labeling and biological evaluation of Androctonus crassicauda scorpion venom are described.Method: In this research, Toxic fraction of this venom was labelled with 99mTc. Radiochemical purity of the labelled toxic fraction was obtained by using chromatographic system. Animal biodistribution studies were performed after injection of labelled compound into normal rats.Results: Radiochemical purity was obtained more than 90%. Biodistribution studies in normal rats showed moderate clearance of blood circulation system. The results of the study indicated that scorpion venom labeling with 99mTc can be a useful tool for the biodistribution and kinetic studies of the venoms for clinical use.

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Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study

Abstract: ABSTRACT

Cited by 6 publications

References 10 publications

Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom

Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom

Biodistribution and Lymphatic Tracking of the Main Neurotoxin of Micrurus fulvius Venom by Molecular Imaging

Radiolabeling of Iranian Androctonus crassicauda Scorpion Venom with Technetium-99m for Biological Studies

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