Esterase polymorphism marking cultivars of Manihot esculenta, Crantz

Resende, Adriana Gazoli; Filho, Pedro Soares Vidigal; Machado, Maria de Fátima Pires da Silva

doi:10.1590/s1516-89132004000300003

Cited by 7 publications

(6 citation statements)

References 18 publications

(13 reference statements)

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“…The number of IRAP/REMAP markers obtained in the current study (431) using only two samples each of Olho Junto, Fecula Branca, IPR União, and Tamboara was higher than the number of molecular markers used in other studies of the cassava genome. Molecular markers, such as isozymes (22) (Chavarriaga-Aguirre et al, 1999); (82) (Resende et al, 2004), RAPD markers (311) (Zacarias et al, 2004), SSR markers (43) (Chavarriaga-Aguirre et al, 1999); (46) (Siqueira et al, 2009), and EST-SSR markers (425) (Kunkaew et al 2011) produced the lowest number of reproducible bands. Although S-SAP is a co-dominant marker retrotransposon, the enzymatic steps may increase the possibility of generating impurities and incorrect digestion (Kalendar and Schulman, 2006).…”

Section: Genetic Diversity Among Cassava Cultivars Using Irap/remap Mmentioning

confidence: 99%

“…Several molecular markers, such as isozymes (Resende et al, 2004), randomly amplified polymorphic DNA (RAPD) (Zacarias et al, 2004), amplified fragment length polymorphisms (AFLP) (Chavarriaga-Aguirre et al, 1999), simple sequence repeats (SSR) (Chavarriaga-Aguirre et al, 1999;Siqueira et al, 2009;Lekha et al, 2010), and simple sequence repeats from expressed sequence tags (EST-SSR) (Kunkaew et al, 2011;Sraphet et al, 2011), have been used to analyze the genetic diversity and relationships among cassava cultivars. However, retrotransposon markers, which are highly promising for phylogenetic studies, gene mapping, and genetic diversity (Kalendar et al, 1999), have not yet been analyzed in cassava cultivars.…”

Section: Introductionmentioning

confidence: 99%

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Development of retrotransposon-based markers IRAP and REMAP for cassava (Manihot esculenta)

et al. 2016

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ABSTRACT. Retrotransposons are abundant in the genomes of plants.In the present study, inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) markers were developed for the cassava genome (Manihot esculenta Crantz). Four cassava cultivars (Fécula Branca, IPR-União, Olho Junto, and Tamboara, two samples per cultivar) were used to obtain IRAP and REMAP fingerprints. Twelve designed primers were amplified alone and in combinations. The 42 IRAP/REMAP primer combinations amplified 431 DNA segments (bands; markers) of which 36 (8.36%) were polymorphic. The largest number of informative markers (16) was detected using the primers AYF2 and AYF2xAYF4. The number of bands for each primer varied from 3 to 16, with an average of 10.26 amplified segments per primer. The size of the amplified products ranged between 100 and 7000 bp. The AYF2 primer generated the highest number of amplified segments and showed the highest number of polymorphic bands (68.75%). Two samples of each cassava cultivar were used to illustrate the usefulness and the polymorphism of IRAP/REMAP markers. IRAP and REMAP markers produced a high number of reproducible bands, and might be informative and reliable for investigation of genetic diversity and relationships among cassava cultivars.

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Section: Genetic Diversity Among Cassava Cultivars Using Irap/remap Mmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of retrotransposon-based markers IRAP and REMAP for cassava (Manihot esculenta)

et al. 2016

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“…This result indicated that esterase isozymes exhibited higher polymorphic patterns than peroxidase among the studied genotypes. Esterases in plants have been intensively explored as biochemical markers due its high allele polymorphism (Carvalho et al, 2003;Resende et al, 2004) and its high number of loci by using the polyacrilamide gel electrophoresis system (Carvalho and Machado, 2004). On the other hand, the electrophoretic bands; for both peroxidase and esterase isozymes, showed wide variation in their intensities ranging from faint to dark, reflecting different activities in the seedling tissues of these genotypes.…”

Section: Genetic Polymorphism Among the Studied Genotypes A Isozymesmentioning

confidence: 99%

Genetic Variation and Phylogenetic Relationships Among Maize Types and Teosinte as Revealed by Isozymes and Rapd Markers

Galal¹,

Motawei²,

Farid³

2013

Egyptian Journal of Genetics and Cytology

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aize or corn (Zea mays L., 2n = 20) is one of the most diverse grain crops found in nature. Selection pressure by both humans and nature has resulted in many different types of corn that vary in physical and chemical characteristics. The most common types of corn include dent, flint, flour, sweet, pop, and waxy corn (Knott et al., 1995). The physical appearance of each kernel type is determined by its pattern of endosperm composition as well as quantity and quality of endosperm (Zilic et al., 2011).

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“…In recent literature addressing the detection of polymorphic loci to estimate the genetic diversity in plants through biochemical markers, the esterase system has been usually and primarily adopted as a genetic-biochemical marker. Many studies (Mangolin et al, 1997;Resende et al, 2004;Souza et al, 2004;Pereira et al, 2001;Orasmo et al, 2007) have demonstrated that the -and -esterases isozymes are produced by several and different loci, that mostly show co-dominant inheritance, being, therefore, an enzyme system suitable to estimate the genetic diversity and to analyze the genetic structure of plant populations.…”

Section: Introductionmentioning

confidence: 99%