Immobilization of alpha-amylase produced by Bacillus circulans GRS 313

Abstract

ABSTRACT

Production of D-myo-inositol(1,2,4,5,6)pentakisphosphate using alginate-entrapped recombinant Pantoea agglomerans glucose-1-phosphatase

“…Considering that the free enzyme lost less than 5% phosphatase activity within 2 h in 20 mM Tris-HCl buffer, pH 8.0, entrapment efficiency was determined to be 82.3 ± 1.2% (n=3) for phytase activity and 81.7 ± 0.9% (n=3) for glucose-1-phosphatase activity. Similar entrapment efficiencies have been reported for papain (Sankalia et al, 2004) and α-amylase (Dey et al, 2003).…”

Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains

“…strain KSM-K38 (Hagihara et al, 2001). These results suggested that the effect of Hg 2+ could vary among the microbial amylases, as observed for other heavy metal ions such as Cd 2+ , Co 2+ , Fe 2+ and Ni 2+ (Pandey et al, 2000;Lo et al, 2001;Dey et al, 2003). In addition, the presence of 5 mM Zn 2+ had a slight stimulatory effect on amylase activity ( (1 mM) showed a slight inhibitory effect on enzyme activity.…”

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

“…A higher decolorization efficiency was observed in the immobilized laccase at all temperatures compared with the free enzyme. This result might be due to the entrapment effect of the beads that protected the enzyme against heat and alkaline denaturation (Dey et al 2003).…”