Incidence of group A rotavirus in urban and rural areas of the city of Londrina-Brazil, from 1995 to 1997

Meneghetti, Ângelo Cesar; Bolognini, Andrea; Lauretti, Flávio; Linhares, Rosa Elisa Carvalho; Santos, Norma; Nozawa, Carlos

doi:10.1590/s1516-89132001000300006

Cited by 3 publications

(4 citation statements)

References 20 publications

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“…The virus is classified into seven serogroups, A to G, based on the VP6 protein which contains the antigenic determinants and is located on the viral inner capsid. Group A which is endemic worldwide is the most common agent of childhood diarrhea, accounting for more than 90% of rotavirus gastroenteritis in humans [9]. Group B, also called adult diarrhea rotavirus (or ADRV), has caused major epidemics of severe diarrhea affecting thousands of people of all ages in China [10], while group C has been associated with rare and sporadic cases of diarrhea in children in many countries, with outbreaks reported in Japan and England [11].…”

Section: Rotavirus (Rv) Is a Non-enveloped Virus With Icosahedral Symmentioning

confidence: 99%

“…Both the VP4 and the VP7 strip contain universal probes for the detection of VP4 and VP7 sequences and probes to distinguish between wild-type G1 and G1 vaccine strain sequences, irrespective of the G or P genotype. While The VP4 strip contains type-specific probes for P [4], P [6], P [8], P [9], and P [10], the VP7 strip contains type-specific probes for G1, G2, G3, G4, G5, G6, G8, and G9.…”

Section: Evaluation Of Rotavirus Diagnostic Techniquesmentioning

confidence: 99%

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Evaluation of Techniques for the Diagnosis of Rotavirus Gastroenteritis in Paediatric Patients

R.K¹,

Oke²,

Anjorin³

et al. 2015

Genes Review

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Rotavirus is the major etiology of acute gastroenteritis in infants and young children worldwide. It has been estimated that about 39% of childhood diarrhoea hospitalizations are caused by the virus and nearly half a million children die from the infections each year especially in developing countries on the Asian subcontinent, Africa, and Latin America where health care facilities are in short supply. Stool specimens collected from the first to fourth days of illness are optimal for detection of the infection. Diagnosis was originally performed using electron microscopy, which is still occasionally used in centers where it is available. Routine diagnosis is however, now performed by antigen detection on feces using commercially available, simple, rapid immunochromatographic dipstick style kits which have superseded the earlier latex agglutination and enzyme immunoassay methods whose usefulness in diagnosis is still relevant. Reverse transcription polymerase chain reaction of feces is also available in some reference and research centers, and is particularly useful for identification of outbreaks due to serogroups other than group A. Similarly viral culture and serology are also available but do not play much roles in diagnosis of acute disease. Rotavirus infection is indeed a public health problem and early, rapid, accurate and reliable diagnostic techniques are essential for effective patient management and infection control.

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Section: Rotavirus (Rv) Is a Non-enveloped Virus With Icosahedral Symmentioning

confidence: 99%

Section: Evaluation Of Rotavirus Diagnostic Techniquesmentioning

confidence: 99%

Evaluation of Techniques for the Diagnosis of Rotavirus Gastroenteritis in Paediatric Patients

R.K¹,

Oke²,

Anjorin³

et al. 2015

Genes Review

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“…2 In Brazil, several studies were conducted to demonstrate the importance of RV in children morbidity and mortality. [3][4][5][6][7][8][9][10] The rotavirus belongs to the Reoviridae family, exhibits icosahedral symmetry, it is not enveloped and was first identified by electron microscopy by Bishop et al 11 The viral particle consists of three layers of protein and the viral genome consists of 11 segments of double-stranded RNA (dsRNA), which encode six structural proteins, VP1-4, VP6 and VP7 and six non-structural proteins, NSP1-6. 12, 13 The VP6 protein, located in the inner capsid of the virus contains the antigenic determinants, which allow their classification into seven serogroups of A to G, with group A being the most common agent of childhood diarrhea.…”

Section: Introductionmentioning

confidence: 99%

“…12, 13 The VP6 protein, located in the inner capsid of the virus contains the antigenic determinants, which allow their classification into seven serogroups of A to G, with group A being the most common agent of childhood diarrhea. 2,6,13,14 RV infection can range from mild diarrhea, with limited duration, to a severe case, with fever, vomiting and dehydration. [15][16][17] Laboratory diagnosis of rotavirus infection is usually performed by antigen detection, using enzyme immunoassay 15 or latex agglutination (LA) techniques, which have a sensitivity and specificity above 90%.…”

Section: Introductionmentioning

confidence: 99%

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

Pereira¹,

Raboni²,

Nogueira³

et al. 2011

Braz J Infect Dis

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Background and objectives: Rotavirus (RV) is the main etiological agent of diarrhea in childhood; its laboratory diagnosis is crucial to guide the clinical management and prevention of its spread. RV immunization was introduced in Brazilian 6-month-old children in 2006. The present study was aimed to evaluate three methodologies used for human RV detection in stool samples obtained from patients hospitalized due to gastroenteritis in a teaching hospital and report the impact of RV immunization in hospitalization by diarrhea. Methods: 293 stool samples collected in the 2001-2008 period were analyzed by enzyme immunoassay (EIA), latex agglutination (LA) and polyacrylamide gel electrophoresis (PAGE). Results: Rotavirus was detected in 34.8% of samples by LA assay, 28.3% of samples by EIA assay and in 25.6% of samples by PAGE assay. Considering the PAGE method as gold standard, the sensitivity, specificity and accuracy of EIA were 94.6%, 94.4% and 94.5%, and to LA were 82.6%, 81.6% and 81.9%, respectively. Conclusions: These results indicate that antigen detection by EIA is a rapid, sensitive and specific method, and could be used in large-scale applications for screening stool samples suspected of RV infection. This study showed decreased incidence of RV infection in hospitalized children prior to the implementation of the national immunization program against RV.

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Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

Pereira

Raboni

Nogueira

et al. 2011

The Brazilian Journal of Infectious Diseases

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Incidence of group A rotavirus in urban and rural areas of the city of Londrina-Brazil, from 1995 to 1997

Abstract: ABSTRACT

Cited by 3 publications

References 20 publications

Evaluation of Techniques for the Diagnosis of Rotavirus Gastroenteritis in Paediatric Patients

Evaluation of Techniques for the Diagnosis of Rotavirus Gastroenteritis in Paediatric Patients

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

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