Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

Pereira, Luciane Aparecida; Raboni, Sônia Mara; Nogueira, Meri Bordignon; Vidal, Luíne Rosele Renaud; Almeida, Sérgio Monteiro de; Debur, Maria C.; Cruz, Cristina Rodrigues

doi:10.1016/s1413-8670(11)70178-7

Cited by 18 publications

(18 citation statements)

References 37 publications

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“…in such cases, RV antigens are routinely investigated and the detection rate of this virus has been approximately 28%. 31 this is a retrospective study aimed to identify the frequency of co-infections of RV and other viruses, like the enteric adenovirus, astrovirus, and norovirus groups, using PCR and Rt-PCR. Furthermore, the clinical and epidemiological characteristics of these patients, and the impact of viral co-infections on their outcome were assessed.…”

Section: Introductionmentioning

confidence: 99%

Viral acute gastroenteritis: clinical and epidemiological features of co-infected patients

Ferreira¹,

Raboni²,

Pereira³

et al. 2012

Braz J Infect Dis

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Section: Introductionmentioning

confidence: 99%

Viral acute gastroenteritis: clinical and epidemiological features of co-infected patients

Ferreira¹,

Raboni²,

Pereira³

et al. 2012

Braz J Infect Dis

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“…Before the introduction of the group A RV (RVA) vaccine in Brazil, the frequency for this virus in the population with AG in our institution was 30%. The number of RVA positive cases has decreased substantially since then (Pereira et al 2011), while other pathogens are now reported more frequently.…”

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confidence: 99%

Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes

Raboni

Damasio

Ferreira

et al. 2014

Mem. Inst. Oswaldo Cruz

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Viral acute gastroenteritis (AG) is a significant cause of hospitalisation in children younger than five years. Group A rotavirus (RVA) is responsible for 30% of these cases. Following the introduction of RVA immunisation in Brazil in 2006, a decreased circulation of this virus has been observed. However, AG remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. We conducted a prospective study of paediatric patients hospitalised for AG. Stool samples were collected to investigate human adenovirus (HAdV), RVA, norovirus (NoV) and astrovirus (AstV). NoV typing was performed by nucleotide sequencing and phylogenetic analysis. From the 225 samples tested, 60 (26%) were positive for at least one viral agent. HAdV, NoV, RVA and AstV were detected in 16%, 8%, 6% and 0% of the samples, respectively. Mixed infections were found in nine patients: HAdV/RVA (5), HAdV/NoV (3) and HAdV/NoV/RVA (1). The frequency of fever and lymphocytosis was significantly higher in virus-infected patients. Phylogenetic analysis of NoV indicated that all of these viruses belonged to genotype GII.4. The significant frequency of these pathogens in patients with AG highlights the need to routinely implement laboratory investigations.

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“…Some diagnostic tools for the confirmation of rotavirus infection in children with gastroenteritis have been used routinely in diagnostic laboratories include enzyme linked immunosorbent assay (ELISA), latex agglutination assay (LA), polyacrylamide gel electrophoresis (PAGE), electron microscopy (EM) and real-time reverse transcriptionpolymerase chain reaction (RT-PCR). [23][24][25][26] However, these diagnostic tools are not always applicable in hospitals with limited laboratory facilities. Moreover, some of these diagnostic tools are expensive and time consuming.…”

Section: Discussionmentioning

confidence: 99%

Severity and treatment level of acute gastroenteritis with rotavirus in children under 5 years in INDONESIA

2018

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Rotavirus diarrhea causing gastroenteritis in children under five years is an important issue that urgently needs to be addressed globally. Delay in management of rotavirus diarrhea can be fatal. Diagnostic tool for detecting rotavirus is, therefore, needed. However, until now the gold standard diagnostic tools are expensive, often not available and affordable in health care settings. The aim of the study was to compare the Vesikari clinical severity score of rotavirus-positive with rotavirus-negative in hospitalized children with acute gastroenteritis. Furthermore, the difference of the level of treatment between rotavirus-positive with rotavirus-negative was also evaluated. This was a cross sectional study that using secondary data from medical records of five general teaching hospital in Indonesia. Subjects were children aged <5 years with acute watery diarrhea admitted to the hospital. Statistical analysis used was chi square test, U-Mann Whitney, and Kruskal Wallis. The results showed that the patient with rotavirus positive have higher dehydration (80.2%) compared to rotavirus negative (70%). The severity level of clinical feature was higher in diarrhea due to rotavirus positive than non rotavirus (11.47± 2.89 vs 10.41 ± 2.70; p<0.000). The level of treatment was higher in rotavirus positive. The majority had treatment plan C (47.7%) higher than plan B and A (45.6% and 30.9%; p<0.050). This was opposite with patient with rotavirus negative that majority had treatment in plan A (69.1%) higher than plan B and C (54.4% and 52.3%) (p<0.001). In conclusion, the severity of gastroentrities in children under 5 years using vesikari score are higher in diarrhea due to rotavirus positive than non rotavirus. The treatment level plan C is higher than plan B and A in diarrhea due to rotavirus. This is opposite with non rotavirus majority have treatment in plan A higher than plan B and C.

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Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

Cited by 18 publications

References 37 publications

Viral acute gastroenteritis: clinical and epidemiological features of co-infected patients

Viral acute gastroenteritis: clinical and epidemiological features of co-infected patients

Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes

Severity and treatment level of acute gastroenteritis with rotavirus in children under 5 years in INDONESIA

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