Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation

Benjamin, Sailas; Pandey, Ashok

doi:10.1590/s1516-89132001000200016

Cited by 64 publications

(33 citation statements)

References 22 publications

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“…According to Iizumi et al, (1990) lipase has a catalytic activity at pH = 7.0 and is stable in the pH range 5.0 to 8.0, directly influencing the activity, solubility and stability of the enzyme. Benjamin and Pandey (2001) also affirm that neutral pH is generally defined as optimal for lipolytic activity.…”

Section: Identification Of Microorganism Strainssupporting

confidence: 56%

Isolation of Lipase-Producing Yeasts from Industrial Oily Residues in Different Culture Media

Paludo¹,

Oliveira²,

Trevisol³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Section: Identification Of Microorganism Strainssupporting

confidence: 56%

Isolation of Lipase-Producing Yeasts from Industrial Oily Residues in Different Culture Media

Paludo¹,

Oliveira²,

Trevisol³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…Action of these two compounds could be attributed to their effect in creating the imbalance of ions in the reaction mixture by absorption or release, respectively. Benjamin and Pandey (2000) reported that the chelator, EDTA and anionic detergent SDS effect 75% of lipase activity and the effect of EDTA is higher than the latter. In this study 80% of activity inhibited by SDS and no lipase activity was observed in the presence of EDTA.…”

Section: Enzyme and Substrate Kineticsmentioning

confidence: 98%

Purification and Characterization of the Lipase from Marine Vibrio fischeri

Ranjitha¹,

Karthy²,

Mohankumar³

2009

IJB

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Lipolytic enzymes from marine microbes have been the focus of intense and growing research. The bioluminescence bacterium Vibrio fischeri was produced lipase enzyme when the medium contained specific substrate. The lipase was purified from the concentrated culture supernatant. The most active fractions were obtained using the technique of precipitation with ammonium sulphate. The precipitated fraction was purified by desalting and ion exchange chromatography. The purified active fraction exhibiting final specific activity of 121U/mg and characterized; the optimum pH was likely between 7 to 8, the optimum temperature was 30°C and about 80 % of activity at 5°C. The enzyme was very stable at the pH 8, at the temperature 30°C. The enzyme was monomeric protein having molecular mass of 57 KDa estimated by native PAGE assay.

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“…However, as shown in this work, it can also be used for obtaining bio-products with high added value such as enzymes. The average cost of the disposal is of $ 0.60/ L, 80% cheaper than olive oil, mostly used substrate for lipase production from microorganisms (Benjamin and Pandey, 2001;Hatzinikolaou et al, 1996;Long et al, 1996) and 50% cheaper than corn oil, which is frequently being cited as an inducer of Fusarium lipases (Maia et al, 2001;Rifaat et al, 2010;"USDA Economic Research Service," 2013). To date, no other work in the literature has reported the use chicken fat to induce production of lipases.…”

Section: Introductionmentioning

confidence: 99%

Chicken fat and inorganic nitrogen source for lipase production by Fusarium sp. (Gibberella fujikuroi complex)

Oliveira¹,

Lima

2014

Afr. J. Biotechnol.

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In this work, we report the production of lipases by a Fusarium sp. isolate (FCLA-MA41) from Atlantic Forest, using chicken fat and association of organic and inorganic nitrogen sources in submerged fermentation to seek economically attractive bioprocess. A 2-level, 4-factor Central Composite Design (CCD) and response surface methodology (RSM) were used to study the influence of the concentrations of chicken fat, (NH 4 ) 2 SO 4 , Triton X-100 and yeast extract as a nutrient supplement in Vogel minimum salt medium. RSM defined the region with the best response as consisting of the following combination of variables: chicken fat 15.0 mL/L, Triton X-100 15.0 g/L, ammonium sulfate 4.5 g/L and yeast extract 1.0 g/L. A validation study was performed according to the described concentrations and produced an enzymatic activity of 4.22 ± 0.35 U/mL. Considering the cost estimates of the nutrient medium optimized for lipase production, the production cost was $US 518.00/million Units of lipase.

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Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation

Abstract: Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC-3

Cited by 64 publications

References 22 publications

Isolation of Lipase-Producing Yeasts from Industrial Oily Residues in Different Culture Media

Isolation of Lipase-Producing Yeasts from Industrial Oily Residues in Different Culture Media

Purification and Characterization of the Lipase from Marine Vibrio fischeri

Chicken fat and inorganic nitrogen source for lipase production by Fusarium sp. (Gibberella fujikuroi complex)

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