Chicken fat and inorganic nitrogen source for lipase production by Fusarium sp. (Gibberella fujikuroi complex)

Abstract

In this work, we report the production of lipases by a Fusarium sp. isolate (FCLA-MA41) from Atlantic Forest, using chicken fat and association of organic and inorganic nitrogen sources in submerged fermentation to seek economically attractive bioprocess. A 2-level, 4-factor Central Composite Design (CCD) and response surface methodology (RSM) were used to study the influence of the concentrations of chicken fat, (NH 4 ) 2 SO 4 , Triton X-100 and yeast extract as a nutrient supplement in Vogel minimum salt medium. RSM defined the region with the best response as consisting of the following combination of variables: chicken fat 15.0 mL/L, Triton X-100 15.0 g/L, ammonium sulfate 4.5 g/L and yeast extract 1.0 g/L. A validation study was performed according to the described concentrations and produced an enzymatic activity of 4.22 ± 0.35 U/mL. Considering the cost estimates of the nutrient medium optimized for lipase production, the production cost was $US 518.00/million Units of lipase.

Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat

“…In cultures of B. lata LBBIO-BL02 in this work there was obtained high activity in comparison with the literature, using as a carbon source chicken fat from disposals of the poultry industry. Although of an animal source, chicken fat has a high content of unsaturated long chain fatty acids (>57 %), 43 % of it is oleic acid; justifying it as good source for lipase production, as observed in other studies conducted by our group for lipase production by Fusarium sp (GFC) (Oliveira and Lima 2014). Besides our work there is no report in the literature of lipase production using chicken oil.…”