Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe

Mello, Marcia O.; Melo, Murilo; Appezzato‐da‐Glória, Beatriz

doi:10.1590/s1516-89132001000200014

Cited by 13 publications

(11 citation statements)

References 23 publications

(28 reference statements)

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“…11). Unlike the reported for P. cincinnata, in Curcuma zedoaria (Melo et al 2001), indirect organogenesis was observed in root segments, although the cellular divisions for callus formation occurred in the cortical parenchyma. Likewise, Peres et al (2001) observed in anatomical analysis of in vitro wild tomato (Lycopersicon peruvianum, L. chilense and L. hirsutum) the formation of buds in the roots, which can also occur direct or indirectly, depending on the genotype used.…”

Section: Root Segments and Seedlingscontrasting

confidence: 83%

In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.

Lombardi

Passos

Nogueira³

et al. 2007

Braz. arch. biol. technol.

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Section: Root Segments and Seedlingscontrasting

confidence: 83%

In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.

Lombardi

Passos

Nogueira³

et al. 2007

Braz. arch. biol. technol.

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“…The calli were yellowish and nodular with morphogenic aspects. Plant can be regenerated from these calli by indirect organogenesis (Mello et al, 2001). According to these authors, histological analysis revealed that callus was formed from hypertrophied cortical parenchyma cells of the explant.…”

Section: Callus Inductionmentioning

confidence: 99%

“…The key to technical and economic feasibility rests on the ability to induce and select genetically stable whole plants or cell cultures that overproduce specific chemicals and the development of scale-up technology that exploits the biological capabilities of plant cells and promotes efficient production (Whitaker & Evans, 1987). To reach these goals, several reports of in vitro culture of species from the Zingiberaceae family have been published (Illg & Faria, 1995;Sharma & Singh, 1997;Borthakur et al, 1998, Shirin et al, 2000Mello et al, 2001;Salvi et al, 2002). This work reports the feasibility of the utilization of tissue culture techniques to establish a protocol for micropropagation and cell suspension culture of Curcuma zedoaria Roscoe, which can be a source for secondary metabolites production.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation and callogenesis of Curcuma zedoaria Roscoe

Miachir¹,

Romani²,

Amaral³

et al. 2004

Sci. agric. (Piracicaba, Braz.)

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Curcuma zedoaria Roscoe (zedoary) is a medicinal properties-bearing Zingiberaceae from which rhizomes are commercially exploited. The objective of this work was to establish an in vitro protocol for micropropagation and callogenesis of Curcuma zedoaria Roscoe as alternative to improve plant production, turning economically feasible the exploitation of its secondary metabolites which present medicinal properties. Micropropagation by using shoot apexes produced by rhizome and from in vitro plants were carried out on Murashige & Skoog medium supplemented with 2.0 mg L -1 benzyl amino purine and 30 g L -1 sucrose. Plantlets were satisfactorily acclimated to greenhouse conditions by using plastic cover for at least 10 days. Treatment with endomycorrhiza at the ex vitro transferring time was beneficial to acclimatization, improving plant growth and development. Callus induction and growth were obtained by inoculating root segments on Murashige & Skoog medium supplemented with 1.0 mg L -1 naphtalene acetic acid and incubation in the dark at 25 ± 2ºC. Cell suspension cultures were established on liquid medium of same chemical composition and same culture conditions and a growth curve was obtained.

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“…Furthermore, it is known that high concentrations of BA usually suppress plant regeneration. 27) The fact that the use of apical double node microshoots resulted in root absence suggests that the addition of NAA was not able to reverse the negative effects of BA on root formation, as in other species. 27,28) The control was the only treatment that promoted the elongation and rooting of the explants.…”

Section: Resultsmentioning

confidence: 98%

In Vitro Propagation of "Jarilla" (Larrea divaricata CAV.) and Secondary Metabolite Production

Palacio

Baeza

Cantero

et al. 2008

Biological & Pharmaceutical Bulletin

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Medicinal value has been attributed to Larrea divaricata CAV. (Zygophylaceae family) by the Indian tribes from South America. 1,2) In Argentina, Hieronymus 3) mentioned the medicinal properties of L. divaricata for a variety of diseases such as venereal disease, tuberculosis, colds, bowel cramps, rheumatism, and also as an antiseptic, expectorant, emetic and diuretic. 2-4) L. divaricata presents abundant flavonoids, 5) mainly quercetine and kaempferol, which are considered to be anticancerigenic agents. [6][7][8] The lignane nordihydroguaiaretic acid (NDGA), isolated from L. divatricata in 1945 by Waller and Gisvold,9) is considered to be a powerful inhibitor of lipoxygenase enzymes, which play an important role in cardiac diseases, asthma, arteriosclerosis, viral infections and cancer. [10][11][12][13][14][15][16] Plant cell culture represents a useful production alternative to direct extraction of valuable secondary metabolites from wild plants. The major advantages of tissue culture techniques over traditional cultivation of wild plants are: (a) useful compounds can be produced under controlled conditions, (b) plants are free of microbes and insects, (c) plants can easily be multiplied to yield specific metabolites, (d) a stable and uniform year-round supply of selected medicinal plants is guaranteed, independent of seasonal variations. [17][18][19][20][21] The aim of this work was to evaluate the conditions for in vitro regeneration of L. divaricata plants as an alternative technique for active compound production. MATERIALS AND METHODSPlant Material L. divaricata seed collection was carried out at Santa María de Punilla, "Sierras de Córdoba," located at 45 km north of Córdoba city, Argentina. This region has a temperate Mediterranean climate, with warm summers and dry winters (not excessively hard). The annual mean temperature is 16°C (the mean maximum and minimum temperatures being 34°C in January and 6°C in July, respectively), with an average annual rainfall of 550 mm. The location's average altitude is 700 m above sea level. 22) A voucher specimen was deposited in the International Herbarium of the National University of Río Cuarto, Argentina (RIOC).Germination Germination rates were assessed in different conditions: 1) control, 2) washing seeds overnight in running tap water, 3) scarification, 4) washing overnight in running tap waterϩscarification, 5) soaking overnight with 0.3 mM gibberellic acid (GA 3 ), 6) soaking overnight with 3 mM GA 3 , 7) soaking overnight with 15 mM GA 3 , 8) soaking overnight with 0.3 mM GA 3 ϩscarification, 9) soaking overnight with 3 mM GA 3 ϩscarification, 10) soaking overnight with 15 mM GA 3 ϩscarification.Seed surfaces were disinfected with ethanol 70% (v/v) for 10 min, followed by sodium hypochlorite 1.5% (v/v) for 15 min, and finally washed three times with sterile distilled water. Sterilized seeds were transferred to culture tubes containing 15 ml of agarized (0.8% w/v) half-strength MS 23) major and minor salt medium with 3% (w/v) sucrose, without plant growth regu...

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Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe

Abstract: Callus was induced from root segments taken from in vitro grown plants of

Cited by 13 publications

References 23 publications

In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.

In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.

Micropropagation and callogenesis of Curcuma zedoaria Roscoe

In Vitro Propagation of "Jarilla" (Larrea divaricata CAV.) and Secondary Metabolite Production

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