Curcuma zedoaria Roscoe (zedoary) is a medicinal properties-bearing Zingiberaceae from which rhizomes are commercially exploited. The objective of this work was to establish an in vitro protocol for micropropagation and callogenesis of Curcuma zedoaria Roscoe as alternative to improve plant production, turning economically feasible the exploitation of its secondary metabolites which present medicinal properties. Micropropagation by using shoot apexes produced by rhizome and from in vitro plants were carried out on Murashige & Skoog medium supplemented with 2.0 mg L -1 benzyl amino purine and 30 g L -1 sucrose. Plantlets were satisfactorily acclimated to greenhouse conditions by using plastic cover for at least 10 days. Treatment with endomycorrhiza at the ex vitro transferring time was beneficial to acclimatization, improving plant growth and development. Callus induction and growth were obtained by inoculating root segments on Murashige & Skoog medium supplemented with 1.0 mg L -1 naphtalene acetic acid and incubation in the dark at 25 ± 2ºC. Cell suspension cultures were established on liquid medium of same chemical composition and same culture conditions and a growth curve was obtained.
coordenador do Curso de Pós-graduação em "Solos e Nutrição de Plantas" pela consideração. amizade. colaboração. Augusto Tave Ao Prof. Otto Jesu Crócomo, pelo incentivo e A Dra. Maria Tereza Vitral de Carvalho, pela A Mogiana Alimentos, colaboraç::ão. na de José iii. Ao Instituto de Pesquisas Tecnológicas (IPT) na pessoa de Antonio Bonomi, pela colaboração. Ao Dr. Raymond Pacovsky, pelo auxilio na correção do texto. A Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (CAPES) pela bolsa de estudos. A equipe da Seção de Microbiologia de Solos do CENA pelo auxilio e amizade. A Seção de Radiobiologia pela colaboração. A Comissión Nacional de Energia At6nica da Argentina, na pessoa do Eng. Agr. Edgardo Brenzoni pela turfa da Patagônia. A FINEP, BOSTID/NAS e EMBRAPA pelo apoio fin�n�Pirn ao projeto.
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