Caracterização da ativação plaquetária nos concentrados de plaquetas por citometria de fluxo

Landi, Evaldo Pasquini; Júnior, José F. C. Marques

doi:10.1590/s1516-84842003000100007

Cited by 9 publications

(5 citation statements)

References 55 publications

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“…Além disso, sabe-se que o estresse mecânico causado pela centrifugação durante o preparo do PRP também pode ativar algumas plaquetas (Gonshor 2002, Landi & Marques Júnior 2003. Nesse sentido, várias pesquisas realizadas em equinos (Smith et al 2006, Schnabel et al 2007, McCarrel & Fortier 2009, Bosch et al 2011, Zandim et al 2013) vem utilizando o PRP sem prévia adição desses agentes ativadores.…”

Section: Discussionunclassified

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

et al. 2014

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ABSTRACT.-Souza M.V., Pinto J.O., Costa M.M., Santos E.C., Garcia S.L.R. & Oliveira L.L. 2014.[Quantification of growth factors in horse skin treated with platelet-rich plasma.] Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas. Pesquisa Veterinária Brasileira 34(6):599-612. Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário s/n, Viçosa, MG 36570-000, Brazil. E-mail: msouza@ufv.br Platelet-rich plasma (PRP) is a product derived from total blood centrifugation, rich in bioactive factors, such as growth factors. Despite largely used in healing processes, there is a controversy whether the therapy is effective in promoting skin healing. The objective of this study was to quantify and compare the concentrations of the factors TGF-β1 and PDGF-BB in PRP, blood plasma and skin, at different phases of the healing process of skin treated or not with PRP. Seven healthy crossbred 16 to 17-year-old geldings (16.14±0.63) were used. Three quadrangular-shaped lesions (6.25cm 2 ) were surgically induced into the right and left gluteal regions of all animals. Twelve hours after induction of the wounds, 0.5mL of the PRP was administered in each of the four edges of the wound in one of the gluteal regions (Treated group = TG) randomly chosen. The contralateral region was used as control (CG). The wounds were submitted to daily cleaning with Milli-Q water, and samples were obtained with a 6mm diameter biopsy Punch. Six skin biopsies were obtained, the first carried out immediately after the production of the wound (T0), and the others 1 (T1), 2 (T2), 7 (T3), and 14 (T4) days after the lesion was induced. The sixth biopsy (T5) was obtained after complete healing of the skin, which occurred at about day 37 (36.85±7.45, CG; 38.85±6.46, TG). EDTA blood samples were also obtained, at all the times mentioned. Quantification of TGF-β1 and PDGF-BB growth factors on the skin, PRP, and blood plasma was carried out by the ELISA technique. Data were statistically analyzed by the t test, Pearson correlation and regression, at a significance level of 5%. No difference was found between the groups in the values of the two growth factors measured on the skin, at the different times. Also, no correlation was found between the amount of growth factors present in the skin and plasma. On the other hand, a positive correlation was observed between PRP and skin in the treated group, for the growth factors TGF-β1 (r=0.31) and PDGF-BB (r=0.38), as well as between both growth factors present in PRP (r=0.81). Considering the growth factor concentrations at T0, the highest skin values (p<0.05) of TGF-β1, in both groups, occurred at T3 and T5. Higher values (p<0.05) of PDGF-BB occurred at T4 (TG) and T5 (CG). No plasma changes occurred at the concentration of these factors in relation to T0, suggest-1 Recebido em 5 de outubro de 2013.Aceito para publicação em 5 de junho de 2014.

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Section: Discussionunclassified

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

et al. 2014

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“…Despite the importance of using the activating agents, it is known that the mechanical stress caused by centrifugation during preparation of the platelet-rich plasma can activate some platelets (Gonshor 2002, Landi & Marques Júnior 2003, which explains the presence of this morphological type in pure PRP evaluated in the present study. In this sense, several experiments have already used the pure PRP, that is, without the previous addition of pharmacological activating agents, in researches conducted both in horses (Smith et al 2006, Schnabel et al 2007, Bosch et al 2009, McCarrel & Fortier 2009) and humans (Mishra & Pavelko 2006, Kajikawa et al 2008.…”

Section: Discussionmentioning

confidence: 77%

Platelet activation: ultrastructure and morphometry in platelet-rich plasma of horses

Zandim¹,

Souza²,

Magalhães

et al. 2012

Pesq. Vet. Bras.

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This study was conducted to investigate the activation ability of the platelet-rich plasma (PRP) by pharmacological agents, as well as to verify the need or not of this activation for therapeutic use. The PRP was obtained from four healthy crossbred geldings aged 13 to 16 years (15±1years), and was processed for observation and quantification of the platelet morphology by using the transmission electron microscopy. All PRP samples were activated with 10% calcium chloride (CaCl2) solution, pure bovine thrombin or associated with CaCl2. The control (pure PRP) was not pharmacologically activated. In the pure PRP samples, 49% of the platelets were classified as state of activation uncertain, 41% as resting, 9% as fully activated and 1% as irreversibly damaged. Treatment with 10% CaCl2 provided a distribution of 54% platelets in state of activation uncertain, 24% as fully activated, 20% as resting, and 2% as irreversibly damaged. The platelet morphology of the bovine thrombin treated samples did not fit into classification adopted, as showing irregular shape with emission of large filamentous pseudopods, appearance of ruptured and whole granules in the remaining cytoplasm and extracellular environment. There was effect of the treatment on the platelet morphology (P=0.03). The 10% CaCl2 is an adequate platelet-activating agent. However, in cases the use of PRP under its liquid form is necessary, the use of pure PRP is recommended, since besides presenting an adequate percentage of fully activated platelets it also has significant amount of the resting type, which can be activated by substances found in the injured tissue.

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“…Hauschild et al (2017), comparing different protocols of PRP, found that depending on the method, it was possible to use PRP up until six hours after production, which is within the limits suggested by Barroso et al (2007), who states that blood derivatives processed in an open circuit should be used within a maximum of six hours. It is therefore suggested that the same recommendation should be followed for PRP, since during its storage, platelets may be activated, what would compromise the biologic function of the product (Landi and Marques Júnior, 2003). According to Weiser (2015), platelet concentration and their aggregation capacity are significantly decreased after 24 hours of storage, and it is therefore not advisable to use platelets after that.…”

Section: Resultsmentioning

confidence: 99%

Pre or perioperative period: when is the best time to produce platelet-rich plasma in dogs?

et al. 2022

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When evaluating published studies on the production of platelet-rich plasma (PRP), it is possible to observe that there is no consensus on when blood should be collected and processed. Some perform blood collection before surgery, prior to the administration of drugs, fluids, or any surgical trauma, while others collect blood during the perioperative period. The present objective was to determine the best moment for PRP production based on the platelet count. Blood samples were collected from 20 healthy female dogs at two distinct moments: before surgery, prior to anesthesia and fluid therapy (M0), and later, in the operation room, after administration of the anesthetics and intravenous fluids (M1). To obtain PRP, blood was centrifuged at 1200 rpm for ten minutes, then at 1600 rpm for ten minutes. Results showed that the blood collected at M1 had a 29% decrease in platelet count compared to M0 (highly significant statistical difference). It is recommended that blood collection, which is an essential step for producing platelet-rich plasma, shall be done during the pre-operative period.

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Caracterização da ativação plaquetária nos concentrados de plaquetas por citometria de fluxo

Cited by 9 publications

References 55 publications

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

Platelet activation: ultrastructure and morphometry in platelet-rich plasma of horses

Pre or perioperative period: when is the best time to produce platelet-rich plasma in dogs?

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