Identification of turkey astrovirus and turkey coronavirus in an outbreak of Poult Enteritis and Mortality Syndrome

Villarreal, Lyb; Assayag,; Brandão, P. E.; Chacón, Jlv; Bunger, And; Astolfi-Ferreira, Claudete S.; Gomes, CR; Jones, R. C.; Ferreira, Ana Paula Garcia

doi:10.1590/s1516-635x2006000200010

Cited by 19 publications

(22 citation statements)

References 20 publications

(21 reference statements)

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“…TAstV2 appears to be the most common among the identified turkey astroviruses, while TAstV1 and avian nephritis virus* an astrovirus first described as responsible for nephritis in chicken (Imada et al, 2000)*have only been detected sporadically in turkey flocks (Pantin-Jackwood et al, 2007, 2008aDomanska-Blicharz et al, 2011). In field surveys, astrovirus is a very common finding in commercial turkey farms with prevalence ranging from 84 to 100% (Pantin-Jackwood et al, 2007, 2008aJindal et al, 2010b) and from 41 to 44% (Villarreal et al, 2006;Domanska-Blicharz et al, 2011). Despite the distribution and relevance of astrovirus infection in turkeys, very few data on pathogenesis and factors influencing the pathogenicity of astrovirus are available (Thouvenelle et al, 1995a, b;Behling-Kelly et al, 2002;Tang et al, 2006;Pantin-Jackwood et al, 2008a).…”

Section: Introductionmentioning

confidence: 99%

Experimental infection of poults and guinea fowl with genetically distinct avian astroviruses

et al. 2012

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Section: Introductionmentioning

confidence: 99%

Experimental infection of poults and guinea fowl with genetically distinct avian astroviruses

et al. 2012

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“…Moreover, astroviruses are linked with enteric disease in humans and young animals such as calves, lambs, pigs, dogs, cats and minks (15,21). The most important infectious disease caused by TAstV are the enteric diseases affecting the digestive tract of commercial poults proposed to result in more economic loss than those affecting any other system reported worldwide, including Brazil (16,17,26,27,28,30,34,35). In addition, no vaccine currently exists for the enteric disease caused by astroviruses, which leads the disease to the bio-security manners to control virus infection and spread (15).…”

Section: Introductionmentioning

confidence: 99%

“…Turkey Coronavirus (TCoV) was described affecting commercial poults in Brazil, suspected of suffer from PEMS- Poult Enteric Mortality Syndrome (34,35). In addition, coronaviruses had often been described as being fastidious.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological aspects of astrovirus and coronavirus in poults in the South Eastern Region of Brazil

Silva¹,

Bonetti²,

Petrocelli³

et al. 2009

Braz. J. Microbiol.

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A survey of Turkey Coronavirus (TCoV) and Astrovirus (TAstV-2) prevalence was carried out from February to December during 2006 year in semiarid region of Brazil, from a turkey producer area, localized in South Eastern of Brazil. To asses the risk factor related to clinical material, climatic condition and type of RT-PCR applied, cloacal swabs (CS), faeces, sera, bursa of Fabricius (BF), thymus (TH) and spleen (SP) and ileum-caeca region were collected from 30-day-old poults suffering of enteritis episode characterized as poult enteritis mortality syndrome (PEMS). The PEMS clinical features were characterized by watery to foamy faeces, light brown-yellow in colour and low mortality rate. Meteorological data (rainfall and relative humidity) observed during along the study presented monthly average temperature ranging from 39.3 and 31.2ºC, precipitation in rainy season from 40 to 270.3 mm/month, and no rain during dry season. Simplex RT-PCR gave odds ratio (OR) values suggesting that ileum-caeca region is at higher chance (OR=1.9; p=0.9741) to have both viral RNA than faeces (OR=1.5; p=0.7319). However, multiplex RT-PCR showed 3.98 (p=0.89982) more chance to give positive results in faeces than CS at dry season. The major risk factors seem to be low rate of humidity and high temperatures at winter, probably responsible for spread, easily, the TCoV and TAstv-2 among the flocks. The positive results of both virus suggested that they can play an important role in enteric disorders, associated to low humidity and high temperatures frequently found in tropical countries.

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“…The fact that the investigation of rotavirus and reovirus in PEMS-associated turkeys was beyond the scope of the current study can be interpreted as a deficiency. On the other hand, the presence of TCoV and HEV has been reported at different rates in both PEMSassociated and clinically healthy turkey flocks in various studies (Carver et al, 2001, Culver et al, 2006Villarreal et al, 2006;Palya et al, 2007;Teixeira et al, 2007;DomanskaBlicharz et al, 2010;Lojkic et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Detection of Astrovirus, Coronavirus and Haemorrhagic Enteritis Virus in Turkeys with Poult Enteritis Mortality Syndrome in Turkey

et al. 2015

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This study was carried out to investigate the presence of turkey astrovirus 2 (TAstV-2), turkey coronavirus (TCoV) and haemorrhagic enteritis virus (HEV) by molecular methods in cloacal swabs collected from both clinically healthy turkey flocks and those associated with poult enteritis mortality syndrome (PEMS) in Turkey. In the reverse transcriptase polymerase chain reaction (RT-PCR) examination of 230 cloacal swabs collected from 23 turkey flocks associated with PEMS, TAstV-2 was detected in 13.4% (31/230) of the animals and in 43.4% (10/23) of the flocks. In addition, this virus was found in two turkeys originating from one of the four clinically healthy flocks. On the other hand, neither TCoV nor HEV were detected in any of the turkey samples examined in this study. In the partial sequence analysis of four randomly selected DNA samples, 96% nucleotide identity was observed between our strains and reference Turkey astrovirus isolated from turkeys in Italy between 2000 and 2004 (sequence accession number DQ381378.1).

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Identification of turkey astrovirus and turkey coronavirus in an outbreak of Poult Enteritis and Mortality Syndrome

Cited by 19 publications

References 20 publications

Experimental infection of poults and guinea fowl with genetically distinct avian astroviruses

Experimental infection of poults and guinea fowl with genetically distinct avian astroviruses

Epidemiological aspects of astrovirus and coronavirus in poults in the South Eastern Region of Brazil

Detection of Astrovirus, Coronavirus and Haemorrhagic Enteritis Virus in Turkeys with Poult Enteritis Mortality Syndrome in Turkey

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