Artificial fertilization of oocytes and sperm activation in pacu: effects of the spermatozoa:oocyte ratio, water volume, and in natura semen preservation

Sanches, Eduardo Antônio; Baggio, Diego Mendes; Piana, Pitágoras Augusto; Souza, Bruno Estevão de; Bombardelli, Robie Allan

doi:10.1590/s1516-35982011000100001

Cited by 28 publications

(15 citation statements)

References 15 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, such assessment is fundamental in artificial fertilization procedures, since the amount of spermatozoa necessary for fertilization is variable among the South American fish species (Bombardelli et al, 2006(Bombardelli et al, , 2013Shimoda et al, 2007;Sanches et al, 2009;Fig. Variations in duration of motility of Steindachneridion parahybae over 173 days throughout the reproductive season (day 0 'zero '-28/10/2009; day 29 -26/11/2009 Sanches et al, 2011;Sanches, 2012). Variations in duration of motility of Steindachneridion parahybae over 173 days throughout the reproductive season (day 0 'zero '-28/10/2009; day 29 -26/11/2009 Sanches et al, 2011;Sanches, 2012).…”

Section: Discussionmentioning

confidence: 99%

Sperm production of Steidachneridion parahybae (Steindachner 1877) and the effect of hormonal induction throughout one reproductive cycle

2015

View full text Add to dashboard Cite

Summary The present study assessed the semen production and spermatozoa characteristics of Steidachneridion parahybae throughout one reproductive cycle in captivity and the effect of the induction of maturity with carp pituitary extract (CPE). Hence, six semen samples were collected from 26 specimens of S. parahybae. The samples were divided into two groups (non‐induced and induced) and tested between October 2009 and April 2010 (water temperature = 21.85 ± 0.77 and 21.85 ± 0.80°C, respectively, and density 705 and 679 g m−2 for non‐induced and induced groups, respectively). The effects of hormonal induction on the studied seminal parameters were not significant (P > 0.05). The mean values and standard error obtained for induced and non‐induced specimens were respectively: relative volume of semen (9.39 ± 1.19 and 9.87 ± 1.03 ml kg−1); sperm motility rate (79.05 ± 1.85 and 81.77 ± 1.54%); motility duration (38.76 ± 0.74 and 39.94 ± 0.80 s); sperm concentration (9.24 ± 0.88 and 8.75 × 109 ± 0.58 × 109 sptz ml−1); pH of the seminal plasma (7.23 ± 0.06 and 7.32 ± 0.05), and osmolality of the seminal plasma (263.21 ± 2.40 and 259.46 ± 3.54 mOsm kg−1). The results indicated that this species presents continuous but variable sperm production, and that hormonal induction does not significantly improve the sperm characteristics of the species.

show abstract

Section: Discussionmentioning

confidence: 99%

Sperm production of Steidachneridion parahybae (Steindachner 1877) and the effect of hormonal induction throughout one reproductive cycle

2015

View full text Add to dashboard Cite

show abstract

“…Inseminating doses are unknown to the S. parahybae and were applied successfully in other species of native South American fish, providing suitable conditions to promote maximum fertilization: Salminus brasiliensis (Sanches et al, 2009;Weingartner, 2010), Rhamdia quelen (Bombardelli et al, 2006), Brycon insignis (Shimoda et al, 2007) and Piaractus mesopotamicus (Sanches et al, 2011a). The injection of suitable inseminating doses during the procedures of artificial fertilization brings important implications, such as higher values of fertilization rates, optimization of gametes and broodstocks, and also serves as basis for cryopreservation programs, allowing the thawing of the exact amount of spermatozoa that is necessary to fertilize the desired amount of oocytes (Bombardelli et al, 2006;Chereguini et al, 1999;Fogli da Silveira et al, 1988;Rurangwa et al, 1998;Sanches et al, 2011a;Sanches et al, 2009;Yasui et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The injection of suitable inseminating doses during the procedures of artificial fertilization brings important implications, such as higher values of fertilization rates, optimization of gametes and broodstocks, and also serves as basis for cryopreservation programs, allowing the thawing of the exact amount of spermatozoa that is necessary to fertilize the desired amount of oocytes (Bombardelli et al, 2006;Chereguini et al, 1999;Fogli da Silveira et al, 1988;Rurangwa et al, 1998;Sanches et al, 2011a;Sanches et al, 2009;Yasui et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

et al. 2016

Self Cite

View full text Add to dashboard Cite

The Steindachneridion parahybae is an endangered catfish from Brazil and strategies applied for gametes optimization are necessary. The aim of this study was to assess inseminating doses and water volume upon the fertilization, hatching rates and percentage of normal larvae in S. parahybae. Was used a randomized design in factorial scheme (4×4) with four inseminating doses: 1.0×10 4 , 1.0×10 5 , 1.0×10 6 , 1.0×10 7 spermatozoa oocyte -1 and four volumes of water: 1, 35, 65 and 95mL of water g -1 of oocytes. The combination of doses and volumes were performed in triplicates (n=48). Each incubator (1.5L of useful volume) with 1g of oocytes was considered as an experimental unit. Significant interaction between inseminating doses and volumes of water to the values of the fertilization rates and quadratic effect of doses and volume for the values of hatching rates were observed. The doses and volumes did not influence the percentage of normal larvae (87.70±5.06%). It is recommended the use of 5.5×10 6 spermatozoa oocyte -1 and 1mL of water g -1 of oocytes during in vitro fertilization procedure. These results allowed us to develop new biotechnological strategies applied to the conservation of S. parahybae.O Steindachneridion parahybae é um bagre ameaçado de extinção no Brasil e estratégias aplicadas para a otimização de gametas são necessárias. O objetivo deste estudo foi avaliar doses inseminantes e volume de água sobre os valores das taxas de fertilização, eclosão e larvas normais em S. parahybae. Utilizando-se um delineamento experimental casualizado em esquema fatorial (4×4), com quatro doses inseminantes: 1,0×10 4 ; 1,0×10 5 ; 1,0×10 6 ; 1,0×10 7 espermatozóides ovócito -1 e quatro volumes de água: 1; 35; 65 e 95mL de água g -1 de ovócitos. As combinações de doses e volumes foram realizadas em triplicatas (n=48). Cada incubadora (1,5L de volume útil) contendo 1g de ovócitos foi considerada como uma unidade experimental. Interações significativas entre doses inseminantes e volumes de água para os valores das taxas de fertilização e efeito quadrático das doses e do volume para os valores das taxas de eclosão foram verificadas. As dosagens e os volumes aplicados não influenciaram no percentual de larvas normais (87,70±5,06). Recomenda-se a aplicação de 5,5×10 6 espermatozoides ovócito -1 e a utilização de 1mL de água.g -1 de ovócitos no procedimento de fertilização artificial in vitro. Estes resultados permitiram desenvolver novas estratégias biotecnológicas aplicadas na conservação do S. parahybae.

show abstract

“…For the male silver catfish, a single dose of 3.0mg CPE kg -1 was applied, and the semen was removed by means of abdominal massage in the cephalocaudal direction after 240 degree-hours. The semen was collected in test tubes and kept cool (12°C) for analysis of sperm and seminal parameters (SANCHES et al, 2011).…”

mentioning

confidence: 99%

Garlic essential oil increases rates of eggs fertilization and hatching of Rhamdia quelen larvae in an artificial incubation system

et al. 2017

Self Cite

View full text Add to dashboard Cite

The objective of this study was to evaluate the effects of garlic (Allium sativum) essential oil on the rates of eggs fertilization and hatching and on the normal development of larvae of artificially incubated silver catfish (Rhamdia quelen). The experiment was carried out using a completely randomized experimental design with four treatments and five replicates. The treatments consisted of introducing garlic essential oil into the incubators, which used a closed system of water recirculation, at concentrations of 0, 1, 3, and 5mg L-1. Rates of eggs fertilization and larval hatching were determined at 10 and 24h after the beginning of eggs hydration. Morphology of larvae was assessed with the aid of a stereo microscope to determine the rate of normal development. The concentration of 5mg L-1 promoted better rates of eggs fertilization and larval hatching. Concentrations of garlic essential oil had a linear effect on eggs fertilization rates and larval hatching. Garlic oil did not influence the morphology of the larvae (P>0.05). It is recommended to use 5mg L-1 of garlic essential oil to promote better rates of eggs fertilization and larval hatching of artificially incubated silver catfish.

show abstract

Artificial fertilization of oocytes and sperm activation in pacu: effects of the spermatozoa:oocyte ratio, water volume, and in natura semen preservation

Cited by 28 publications

References 15 publications

Sperm production of Steidachneridion parahybae (Steindachner 1877) and the effect of hormonal induction throughout one reproductive cycle

Sperm production of Steidachneridion parahybae (Steindachner 1877) and the effect of hormonal induction throughout one reproductive cycle

Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

Garlic essential oil increases rates of eggs fertilization and hatching of Rhamdia quelen larvae in an artificial incubation system

Contact Info

Product

Resources

About