Analysis of the growth pattern of Vero cells cultured on dense and porous poly (L-Lactic Acid) scaffolds

Santos, Arnaldo Rodrigues; Barbanti, Samuel Hilsdorf; Duek, Eliana Aparecida de Rezende; Wada, Maria Lúcia Furlan

doi:10.1590/s1516-14392009000300002

Cited by 14 publications

(9 citation statements)

References 20 publications

(19 reference statements)

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“…The initial cell adhesion to the untreated PLGA membrane was poor. The cells were small, rounded and separated from each other (Figure 2a), which is in accordance with other studies using Vero cells seeded on untreated scaffolds 1,16,[29][30][31] . In contrast, it was noticed that Vero cells cultivated for 2 hours on plasma-treated PLGA presented higher density of adhered cells in close contact with each other, spread on the membrane surface.…”

Section: Scanning Electron Microscopy (Sem)supporting

confidence: 92%

Benefits of oxygen and nitrogen plasma treatment in Vero cell affinity to poly(lactide-co-glycolide acid)

et al. 2013

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Cell adhesion on materials surface is critical because this phenomenon occurs before other events, as cell spreading, cell migration and cell differentiation. It is commonly accepted that the adhesion of cells on solid substrate is influenced by several substratum surface properties, such as wettability, surface charge, roughness and topography. Plasma technique is a convenient method for modifying surface properties of materials without affecting physical properties. In this study, poly(lactide-co-glycolide), PLGA, membranes were modified by oxygen and nitrogen plasma to improve polymer hydrophilicity and verify their effect on Vero cells culture. The PLGA membranes, which were characterized by SEM and contact angle, showed increased surface rugosity and narrower contact angles. Cell adhesion, cytotoxicity assay, SEM and cytochemistry analysis showed that plasma treatment was beneficial to cell growth by improving cell-polymer interaction.

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Section: Scanning Electron Microscopy (Sem)supporting

confidence: 92%

Benefits of oxygen and nitrogen plasma treatment in Vero cell affinity to poly(lactide-co-glycolide acid)

et al. 2013

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“…The cells were cultured in Medium 199 (Lonza Group Ltd, USA) and supplemented with 10% fetal calf serum (FCS, by Nutricell Nutrientes Celulares, Campinas, SP, Brazil), at a temperature of about 37ºC. Vero cells are recommended for cytotoxicity and cellsubstratum interactions studies, with biomaterial researches 6,7 . The concentration of 1x105cells/ml was inoculated on 24-well culture plates (Corning) on the different biomaterial compositions.…”

Section: Characterizationsmentioning

confidence: 99%

Fibrous PCL/PLLA Scaffolds Obtained by Rotary Jet Spinning and Electrospinning

et al. 2018

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Rotary jet spinning (RJS) and electrospinning are techniques to obtain fibrous scaffolds. RJS is a simple method, which fabricates three-dimensional fibers by exploiting a high-speed rotating nozzle, creating a polymer jet which stretches until solidification, and does not require high voltage. In opposite, electrospinning technique needs the presence of an external electric field to create fiber from the polymeric jet solution. This article investigates both processes using two different biocompatible polymers: Poly(L-lactic acid) (PLLA) and Poly(ε-caprolactone) (PCL). Samples were characterized by scanning electron microscopy, thermogravimetric analysis, differential scanning calorimeter, and Fourier-transform infrared spectroscopy. Morphological observations showed the efficiency of both techniques in obtaining nanofibers. Thermal analyses of data indicate immiscible property of different blends and the total solvent evaporation. In vitro cytocompatibility test showed that RJS and electrospinning samples exhibited good cytocompatibility. Based on these results, it may be concluded that the fibers obtained with both technologies are non-cytotoxicity and with good biocompatibility, and might be suitable for applications as scaffold for cell growth.

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“…In particular, poly(lactic acid) (PLA) is a popular polyester material that is used in tissue engineering because of its excellent biocompatibility and mechanical properties. Good adherence and differentiation properties have been observed for osteoblasts cultured on PLA membranes, Santos et al (2009), Liu et al (2004).…”

Section: Introductionmentioning

confidence: 90%

Lactone-layered double hydroxide networks: Towards self-assembled bioscaffolds

Zhou

McCarthy

Soutis

et al. 2018

Applied Clay Science

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This paper describes the conversion of a layered anionic initiator (carbonate-intercalated layered double hydroxide, (LDH-carbonate)) into a self-assembled resin-embedded network during the insitu polymerisation of one or more lactone monomers using the LDH-carbonate as the sole initiator. Uniquely in this paper, no long-chain acid intercalant is present in the LDH-carbonate to act as an additional initiator species, and this is the first known report of a copolymerisation of X-ray diffraction (XRD) is used to verify the morphology of the insoluble phases. This demonstrates that the molecular sheets of the LDH-carbonate are fully dismantled during the polymerization. Porous, network morphology is established for some of the insoluble phase structures using scanning electron microscopy (SEM). This indicates potential suitability of these self-assembled insoluble phase materials as bioscaffolds for artificial cell growth. Nuclear magnetic resonance spectrometry (NMR) was used to determine the ratio of ester to acidic carbonyls in the insoluble phase. Energy dispersive X-ray spectroscopy was also used to determine the ratio of magnesium to aluminium in the insoluble phases.

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Analysis of the growth pattern of Vero cells cultured on dense and porous poly (L-Lactic Acid) scaffolds

Cited by 14 publications

References 20 publications

Benefits of oxygen and nitrogen plasma treatment in Vero cell affinity to poly(lactide-co-glycolide acid)

Benefits of oxygen and nitrogen plasma treatment in Vero cell affinity to poly(lactide-co-glycolide acid)

Fibrous PCL/PLLA Scaffolds Obtained by Rotary Jet Spinning and Electrospinning

Lactone-layered double hydroxide networks: Towards self-assembled bioscaffolds

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