Benefits of oxygen and nitrogen plasma treatment in Vero cell affinity to poly(lactide-co-glycolide acid)

Abstract

Cell adhesion on materials surface is critical because this phenomenon occurs before other events, as cell spreading, cell migration and cell differentiation. It is commonly accepted that the adhesion of cells on solid substrate is influenced by several substratum surface properties, such as wettability, surface charge, roughness and topography. Plasma technique is a convenient method for modifying surface properties of materials without affecting physical properties. In this study, poly(lactide-co-glycolide), PLGA, membranes were modified by oxygen and nitrogen plasma to improve polymer hydrophilicity and verify their effect on Vero cells culture. The PLGA membranes, which were characterized by SEM and contact angle, showed increased surface rugosity and narrower contact angles. Cell adhesion, cytotoxicity assay, SEM and cytochemistry analysis showed that plasma treatment was beneficial to cell growth by improving cell-polymer interaction.

Comparative Effects of Non-Thermal Atmospheric Pressure Plasma on Migration and Invasion in Oral Squamous Cell Cancer, by Gas Type

“…MMP-2/9 activity was examined using gelatin zymography as described previously. 17 The cells were treated with He, Ar, or N 2 plasma for 5 min and incubated for an additional 24 h. The supernatant (100 µL) from each sample was mixed with 1 µL of 100 mM APMA (4-aminophenylmercuric acetate, Sigma-Aldrich, St. Louis, MO, USA) and the samples were activated for 1 h at 37℃ incubator. Next, each sample was placed in sample buffer (without mercaptoethanol) for 10 min and electrophoresed in sodium dodecyl sulfate (SDS) page gelatin gels at 125 V for 120 min at room temperature using a Novex Xcell II system (Thermo Scientific, Waltham, MA, USA).…”

“…Many studies showed that biological effects of NTP vary by their gas types, their combinations, treatment conditions, and treated cell types. 13 14 15 16 17 Different plasma gas sources were used depending on various research groups and indications including anti-infection, 18 19 wound healing, 20 and anticancer treatment. 3 12 However, the inhibitory effects of NTP composed of different gases on cancer migration and invasion have not been directly compared until now.…”

A simple and robust method for pre-wetting poly (lactic-co-glycolic) acid microspheres

“…PLGA membranes modified by oxygen and nitrogen plasma have also been shown to present increased surface rugosity that supported attachment of Vero cells. 6 , 30 …”

“…A number of approaches including ethanol immersion, 3 , 4 chemical modification with alkaline solutions (e.g. NaOH) 5 and plasma oxygen 6 , 7 are demonstrated as viable techniques for improving the hydrophilicity, pre-wetting or hydrophilisation of PLGA. The ethanol-mediated pre-wetting method involves the exchange of ethanol for culture medium in microsphere pores to eventually submerge these constructs and enable contact with cells, whereas hydrophilisation involves hydrolysis of the microsphere surface with strong acids or alkalis ( Figure 1 ).…”

Osteoconduction of Unrestricted Somatic Stem Cells on an Electrospun Polylactic-Co-Glycolic Acid Scaffold Coated with Nanohydroxyapatite

“…Of note, synthetic polymers used for scaffold production are hydrophobic, and oxygen plasma treatment of the PLGA scaffold is applied to modify the surface for increasing their hydrophilicity and biocompatibility of PLGA samples [Esposito et al, 2013;Intranuovo et al, 2016]. Oxygen plasma treatment could modify the surface of polymers without changing their mechanical and physical properties [Pappa et al, 2015].…”