Análise tecidual e celular das brânquias de Oreochromis niloticus L. tratadas com extrato etanólico bruto e frações das folhas da pitanga (Eugenia uniflora L.) - Myrtaceae

Fiuza, Tatiana de Sousa; Silva, P.C.; Paula, José Realino de; Tresvenzol, Leonice Manrique Faustino; Souto, M.E.D.; Sabóia‐Morais, Simone Maria Teixeira de

doi:10.1590/s1516-05722011000400003

Cited by 7 publications

(6 citation statements)

References 15 publications

(7 reference statements)

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“…Therefore, these structural alterations to the gills of C. macropomum were caused by the toxicity of the essential oil of L. alba and were boosted by the diluent of this essential oil. Similar action by different diluents has been described in relation to Heteropneustes fossilis exposed to an extract from Azadirachta indica (Kumar et al, 2010) and in relation to O. niloticus exposed to an extract from Eugenia uniflora (Fiuza et al, 2011).…”

Section: Discussionsupporting

confidence: 68%

“…In this study, fish exposed to 100 and 150 mg/L of the essential oil of L. alba for 30 min showed low efficacy against monogenoideans but also Table 5 Mean alteration value (MAV) and histopathological alteration index (HAI) of the gills of Colossoma macropomum exposed to the essential oil of Lippia alba. there were severe structural alterations to the gills, which are organs that participate in respiration, osmoregulation and excretion in fish (Fiuza et al, 2011;Kumar et al, 2010). Moreover, intense parasitism by monogenoideans and by I. multifiliis was observed in all the treatments, which also influenced the tissue integrity of the gills.…”

Section: Discussionmentioning

confidence: 99%

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Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

Soares

Neves²,

Oliveira

et al. 2016

Aquaculture

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This study investigated the in vivo and in vitro antiparasitic effects of the essential oil of Lippia alba and the bloodrelated and histopathological alterations that it causes in Colossoma macropomum. In the in vitro trial, the anthelminthic effects of 160, 320, 640, 1280 and 2560 mg/L of the essential oil were tested against monogenoideans (Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri) of the gills of this fish, which are its natural parasites. The concentrations of 1280 mg/L and 2560 mg/L showed 100% efficacy after 20 min of exposure to the essential oil, while at lower concentrations this efficacy against the gill monogenoideans only occurred after 2-3 h of in vitro exposure. However, in the controls, mortality of all of these monogenoideans only occurred after 9 h. A total of 240 fry were distributed into four treatments (20 fish per repetition) and three repetitions were used in the in vivo trial for baths with 100 and 150 mg/L of the essential oil of L. alba, for 30 min. The efficacy in this trial against Ichthyophthirius multifiliis in fish exposed to 100 and 150 mg/L of the essential oil was 40.7% and 50.3%, respectively. However, for monogenoideans, there was efficacy of 14.0% only in the fish exposed to 100 mg/L of the essential oil used. Moreover, the fish exposed to these concentrations of the essential oil presented increased plasma glucose levels, thus denoting signs of stress. Severe lesions such as hyperplasia, fusion of the lamellar epithelium, capillary dilatation, epithelial detachment, lamellar aneurysm, epithelial rupture with hemorrhage, congestion, edema, necrosis, mucous cell proliferation, chloride cells and lamellar hypertrophy were observed in the gills of the fish exposed to 100 and 150 mg/L of the essential oil of L. alba. Alterations to total protein levels, hemoglobin, hematocrit, red blood cell number, thrombocytes number, white blood cell number, lymphocytes, eosinophils and blood neutrophil number was also observed in these fish. The essential oil of L. alba showed great potential for antiparasitic treatment, given that it had high in vitro efficacy against monogenoideans and in vivo efficacy against the protozoon I. multifiliis. Because of the low concentrations of the essential oil (100 and 150 mg/L) that were tolerated by the fish and thus could be used in the therapeutic baths, the efficacy against monogenoideans was low. This indicates that there is a need for additional strategies for using this essential oil in antiparasitic treatments, since the concentrations that eliminate these ectoparasites are toxic for the hosts. Lastly, this was the first study on the antiparasitic activity of L. alba. Statement of Relevance The manuscript entitled "Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects", represents original article on use of the essential oil of Lippia alba on ectoparasites of tambaqui, an important finfish of Amazon regio...

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

Soares

Neves²,

Oliveira

et al. 2016

Aquaculture

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“…Fish gills are organs responsible for the breathing, osmoregulation and excretion in fish (Fiuza et al, 2011;Kumar et al, 2010), (C) Monogenoidean (*) and regions with epithelium hyperplasia with lamellar fusion (arrows), in the gill from the control treatment with water. (D) Epithelium hyperplasia generalized with regions of lamellar fusion and detachment of the lamellar epithelium in the gills of fish exposed to 20 mg/L.…”

Section: Discussionmentioning

confidence: 99%

Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae)

Soares

Neves²,

Ferreira³

et al. 2017

Veterinary Parasitology

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In vivo and in vitro antiparasitic activity of the essential oil of Lippia sidoides and blood and histological alterations were assessed in Colossoma macropomum (tambaqui). Essential oil concentrations of 10, 20, 40, 80, 160 and 320mg/L were assayed in vitro against monogenoideans Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri from fish gills. Lippia sidoides essential oil concentrations of 320 and 160mg/L were 100% effective against monogenoideans in 10min and 1h of exposure, respectively. However, the effectiveness of 100% concentrations of 80mg/L and 40mg/L occurred in 3 and 6h, respectively. In the in vivo tests, juvenile fish were submitted to 60min of baths with 10mg/L and 15min of baths with 20mg/L of the essential oil of L. sidoides. These therapeutic baths were not efficient against Ichthyophthirius multifiliis, and monogenoideans present in the gills of C. macropomum. In addition, 10 and 20mg/L of the essential oil of L. sidoides caused an anesthetic effect on the fish and did not influence total glucose and protein plasma levels; however, it decreased the number of total erythrocytes in fish exposed to the higher concentration of this essential oil. Severe alterations and irreversible damage were observed in the fish gills just after L. sidoides essential oil baths and after 24h of recovery. The most recurrent lesions found were hyperplasia and fusion of the lamellar epithelium, vasodilation, detachment of the gill epithelium and lamellar aneurism, epithelial breakdown with hemorrhage, congestion, edema and necrosis, proliferation of the mucous cells and chloride cells and lamellar hypertrophy. Therefore, since the essential oil of L. sidoides has in vitro antiparasitic activity and low concentrations of it have shown toxic effects, the bioactive potential of its main chemical components should be investigated, as well as more efficient forms of its administration in therapeutic baths in order to eliminate fish parasites.

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“…Lesões semelhantes foram observadas quando as tilápias foram expostas a agentes tóxicos, como a amônia (Benli & Köksal, 2005;Benli et al, 2008), o cádmio (Garcia-Santos et al, 2007), quando em deficiência de vitamina C (Leonardo et al, 2001), e quando tratadas com extrato e frações da Eugenia uniflora L. (Fiuza et al, 2011).…”

Section: Discussionunclassified

Efeito do extrato etanólico bruto e das frações da Hyptidendron canum (Pohl ex Benth.) Harley em brânquias de Oreochromis niloticus L.

Fiuza

Silva

Paula

et al. 2015

Rev. bras. plantas med.

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RESUMO:Alguns problemas relacionados à criação intensiva de peixes estão mais evidentes devido ao aumento da aquicultura em todo o mundo, destacando-se os distúrbios nutricionais e o aumento de doenças nos sistemas de produção. Com isso as pesquisas envolvendo produtos derivados de plantas vem ganhando espaço nessa área. A Hyptidendron canum é uma planta utilizada na medicina popular como antimalárica, anti-inflamatória, antiulcerativa e anti-hepatotóxica. Objetivou-se, neste trabalho, avaliar os efeitos celulares e teciduais do extrato etanólico bruto e das frações hexano, clorofórmio e acetato de etila da H. canum nas brânquias de Oreochromis niloticus L. Para isso, o extrato etanólico e as frações foram administrados no peixe através da ração. Após 24 horas da ingestão da ração, os peixes foram sacrificados e o segundo arco branquial de cada um foi processado histologicamente e corado com tricômico de Masson e Hematoxilina e Eosina (HE). Pela análise qualitativa das brânquias ao microscópio de luz, observou-se que o extrato bruto e as três frações promoveram, em diferentes intensidades nas lamelas, descamação e destacamento do epitélio respiratório, alteração da curvatura, desorganização do eixo, hiperplasia celular do tecido epitelial interlamelar e vasodilatação nas lamelas e no vaso central do filamento. Os resultados deste experimento mostraram que o extrato etanólico bruto e as três frações de H. canum promoveram processos inflamatórios e/ou lesões sistêmicas, dose dependente para O. niloticus. Palavras-chave:Cerrado, plantas medicinais, tilápia, toxicidade. ABSTRACT: Effect of the crude ethanol extract and fractions of Hyptidendron canum(Pohl ex Benth.) Harley on the gills of Oreochromis niloticus L. Some problems related to intensive fish farming are more evident because of the increased aquaculture worldwide, and we highlight the nutritional disorders and the increase of diseases in the production systems. Thus, research involving plant-derived products has been increasing in this area. The Hyptidendron canum is a plant used in folk medicine as an antimalarial, anti-inflammatory, anti-ulcer and antihepatotoxic agent. This study proposes to evaluate the cellular and tissue effects of the crude ethanol extract and the hexane, chloroform and ethyl acetate fractions of H. canum on the gills of Oreochromis niloticus L. The ethanol extract and fractions were administered through the feed of the fish. Twenty-four hours later, the fish were sacrificed and their second gill arch dissected. Histological analyses were performed using Masson's trichrome and Haematoxylin and Eosin (H&E). The qualitative analysis of the material showed that the crude extract and the three fractions caused varying degrees of peeling and detachment of the lamellar respiratory epithelium, alterations in the curvature and axis of the gill, cell hyperplasia in the interlamellar epithelium and lamellar vasodilation, as well as vasodilation in the central vessel of the filament. The results of this experiment showed that the crude ethanol...

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Análise tecidual e celular das brânquias de Oreochromis niloticus L. tratadas com extrato etanólico bruto e frações das folhas da pitanga (Eugenia uniflora L.) - Myrtaceae

Cited by 7 publications

References 15 publications

Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae)

Efeito do extrato etanólico bruto e das frações da Hyptidendron canum (Pohl ex Benth.) Harley em brânquias de Oreochromis niloticus L.

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