Thrombospondin in protein malnutrition induced hypoplasia

Abstract

OBJECTIVE: The objective of the present study was to measure the concentration of bone marrow extracellular matrix thrombospondin in mice, following hypoplasia induced by protein malnutrition. METHODS: Two-month-old male Swiss mice were submitted to protein malnutrition by way of a low-protein diet containing 4.0% casein until they lost 20.0% of their original body weight, while the control group mice were fed 14.0% casein for 15 days. The bone marrows of the animals were aspirated and transferred to phosphate-buffered saline tubes for extraction. The extracellular matrix protein was analyzed by 7.5% SDS-PAGE and thrombospondin by Enhanced Chemiluminescence Light Western blotting. RESULTS: The amount of thrombospondin was 30% higher in the undernourished samples when compared to the control samples. CONCLUSION: This study suggests that the hypoplasia induced by protein malnutrition probably alters the functioning of the bone marrow microenvironment resulting in a higher thrombospondin concentration.

Pesquisa de isoformas da fibronectina em culturas de longa duração de estroma medular de camundongos submetidos à desnutrição proteica

“…Isso porque quando as células tronco/progenitoras hematopoéticas dos animais desnutrido foram plaqueadas em uma matriz integra de fibronectina, estas células responderam de forma adequada, uma vez que estas proliferam de forma similar às células dos animais controles. (VITURI, et al ., 2000(VITURI, et al ., , 2005XAVIER, et al ., 2007). Em experimentos futuros serão realizados o seqüenciamento das proteínas presentes no gel bidimensional das culturas de longa duração, para identificação das proteínas da matriz extracelular e também a investigação da presença de metalaproteinases que estão envolvidas com o remodelamento da matriz, bem como os inibidores de metalaproteinases.…”