Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae)

Rocha, Marília de França; Melo, Natoniel Franklin de; Souza, Maria José de

doi:10.1590/s1415-47572011000200008

Cited by 9 publications

(9 citation statements)

References 29 publications

(51 reference statements)

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“…The position of the 18S rDNA site in the proximal region of chromosome pair 9 in C. borelli and Amblytropidia sp corresponds to that observed in five other gomphocerine species and in 16 species of the family Acrididae Loreto et al, 2008;Rocha et al, 2011;Bueno et al, 2013), all with a karyotype of 2n = 23,X0. This finding indicates that the ancestral site was located on S9 bivalent in of Neotropical gomphocerine grasshoppers (Loreto et al, 2008).…”

Section: Discussionsupporting

confidence: 71%

“…Molecular cytogenetic studies using fluorescence in situ hybridization (FISH) with repetitive DNA probes, such as rDNA and histone genes, have been carried out on many grasshopper species, particularly of the family Acrididae Loreto et al, 2008;Cabrero et al, 2009;Cabral-de-Mello et al, 2011a;Oliveira et al, 2011;Rocha et al, 2011;Jetybayev et al, 2012;Bueno et al, 2013;Palacios-Gimenez et al, 2013). The objective of physical mapping of these and other repetitive DNA sequences is to provide information on chromosome structure and gene distributions in a range of species that would serve as a basis for phylogenetic studies Cabrero et al, 2009;Cabral-de-Mello et al, 2011a,b;Jetybayev et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Physical mapping of 18S and 5S rDNA loci and histone H3 gene in grasshopper species of the subfamily Gomphocerinae (Acrididae)

Silva-Neto¹,

Bernardino²,

Loreto³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. In this study, fluorescence in situ hybridization (FISH) analysis was used to determine and compare the numbers and chromosomal locations of two multigene families (rDNA and histone H3) in four Neotropical species of gomphocerine grasshoppers. FISH using the 18S rDNA probe identified a single site on the S9 chromosome of Amblytropidia sp and Cauratettix borelli, a single site on chromosome M6 of Compsacris pulcher, and two sites (chromosomes L1 and L2) in Orphulella punctata. By contrast, FISH with a 5S rDNA probe identified dispersion of this sequence in the genomes of the four species, with evidence of intraspecific variations. Amblytropidia sp had six to eight FISH signals on autosomal chromosomes, while C. pulcher exhibited a signal only on the M5 bivalent. The histone H3 gene was less variable and was restricted to a single pair in all species. The conservation of the numbers and locations of 18S rDNA and H3 genes in conjunction with data from the literature was useful 15009 Physical mapping in Gomphocerinae species ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 15008-15015 (2015) for evaluating karyotype evolution in this subfamily. The variation in the number and sizes of 5S rDNA sites indicates a process of recent dispersion that might have been mediated by transposition.

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Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Physical mapping of 18S and 5S rDNA loci and histone H3 gene in grasshopper species of the subfamily Gomphocerinae (Acrididae)

Silva-Neto¹,

Bernardino²,

Loreto³

et al. 2015

Genet. Mol. Res.

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“…The occurrence of DAPI + heterochromatin (AT-rich) in Tropidacris cristata grandis also differentiates it from Tropidacris collaris , since the karyotype of the latter species does not have AT-rich regions. The same is true for the various members of this family studied to date (Loreto et al 2008, Rocha et al 2011, Bueno et al 2013). Likewise, the DAPI + blocks of the chromosomes M 3 and M 5 of Tropidacris cristata grandis were syntenic with CMA 3 + blocks.…”

Section: Discussionmentioning

confidence: 59%

Spreading of heterochromatin and karyotype differentiation in two Tropidacris Scudder, 1869 species (Orthoptera, Romaleidae)

Rocha¹,

Pine²,

Oliveira³

et al. 2015

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Tropidacris Scudder, 1869 is a genus widely distributed throughout the Neotropical region where speciation was probably promoted by forest reduction during the glacial and interglacial periods. There are no cytogenetic studies of Tropidacris, and information allowing inference or confirmation of the evolutionary events involved in speciation within the group is insufficient. In this paper, we used cytogenetic markers in two species, Tropidacris collaris (Stoll, 1813) and Tropidacris cristata grandis (Thunberg, 1824), collected in different Brazilian biomes. Both species exhibited 2n=24,XX for females and 2n=23,X0 for males. All chromosomes were acrocentric. There were some differences in the karyotype macrostructure, e.g. in the chromosome size. A wide interspecific variation in the chromosome banding (C-banding and CMA3/DAPI staining) indicated strong differences in the distribution of repetitive DNA sequences. Specifically, Tropidacris cristata grandis had a higher number of bands in relation to Tropidacris collaris. FISH with 18S rDNA revealed two markings coinciding with the NORs in both species. However, two analyzed samples of Tropidacris collaris revealed a heterozygous condition for the rDNA site of S10 pair. In Tropidacris collaris, the histone H3 genes were distributed on three chromosome pairs, whereas in Tropidacris cristata grandis, these genes were observed on 14 autosomes and on the X chromosome, always in terminal regions. Our results demonstrate that, although the chromosome number and morphology are conserved in the genus, Tropidacris cristata grandis substantially differs from Tropidacris collaris in terms of the distribution of repetitive sequences. The devastation and fragmentation of the Brazilian rainforest may have led to isolation between these species, and the spreading of these repetitive sequences could contribute to speciation within the genus.

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“…The sex-determination pattern considered the ancestor of grasshoppers was present in specimens of Abracris flavolineata , with males with the X0 and females with the XX sex chromosome system. In Acrididae, some species, such as Rhammatocerus brasiliensis (Bruner, 1904), R. brunneri (Giglio-Tos, 1895), R. palustris Carbonell, 1988, R. pictus (Bruner, 1900), Orthoscapheus rufipes (Thunberg, 1824), among others, show a karyotype similar to that of Abracris flavolineata , with 2n = 23,X0 and 24,XX, being considered the ancestral pattern of the family (White 1973; Loreto et al 2008b; Rocha et al 2011). …”

Section: Resultsmentioning

confidence: 99%

“…Specific C-banding techniques for the identification and classification of chromosomes have been applied more often in Abracris flavolineata species (De Geer, 1773) (Cella and Ferreira 1991, Rocha et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Chromosome mapping in Abracris flavolineata (De Geer, 1773) (Orthoptera) from the Iguaçu National Park – Foz do Iguaçu, Paraná, Brazil

Pine¹,

Gallo²,

Silva³

et al. 2017

CCG

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In this paper, we present the cytomolecular analysis of a population of Abracris flavolineata collected in the largest fragment of the Brazilian Atlantic forest, the Iguaçu National Park. The diploid number in males was 23 (22+X0), with two large pairs (1–2), 7 medium (3–9), 2 small (10–11) and the X chromosome of medium size. Heterochromatic blocks were evident in the pericentromeric regions of all chromosomes. Heterogeneity in the distribution of heterochromatin was observed, with a predominance of DAPI+ blocks. However, some chromosomes showed CMA3+ blocks and other DAPI+/CMA3+ blocks. The 18S rDNA sites were distributed on the short arms of 5 pairs. In two of these pairs, such sites were in the same chromosome bearing 5S rDNA, and one of the bivalents, they were co-located. Histone H3 genes were found on one bivalent. The results added to the existing cytogenetic studies provided evidence of great karyotypic plasticity in the species. This pliancy may be the result of vicariant events related to the geographical distribution of different populations of A. flavolineata.

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Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae)

Cited by 9 publications

References 29 publications

Physical mapping of 18S and 5S rDNA loci and histone H3 gene in grasshopper species of the subfamily Gomphocerinae (Acrididae)

Physical mapping of 18S and 5S rDNA loci and histone H3 gene in grasshopper species of the subfamily Gomphocerinae (Acrididae)

Spreading of heterochromatin and karyotype differentiation in two Tropidacris Scudder, 1869 species (Orthoptera, Romaleidae)

Chromosome mapping in Abracris flavolineata (De Geer, 1773) (Orthoptera) from the Iguaçu National Park – Foz do Iguaçu, Paraná, Brazil

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