The acrocentric macro B chromosomes of Rhammatocerus brasiliensis (Acrididae, Gomphocerinae) and Xyleus discoideus angulatus (Romaleidae, Romaleinae) are highly similar to the X chromosome in each species in terms of morphology, size, and pycnosis. However, the results of FISH experiments using 45S and 5S rDNA probes suggest that in both species the B chromosomes are most likely of autosomal origin. In R. brasiliensis, the B chromosome presented 5S rDNA but not 45S rDNA, in resemblance to the L(2), L(3), M(5) and S(11) autosomes, but the X chromosome lacks both rDNA families. In X. d. angulatus, 45S rDNAs is absent from the B chromosome, whereas the X chromosome contains one of the two 45S rDNA clusters in the genome. The occurrence of B chromosomes in all nine R. brasiliensis populations analyzed indicates that they are widely distributed in Northeastern Brazil, and the small amount of interpopulation variation found for B chromosome prevalence suggests the existence of high gene flow, presumably due to the abundance of this grasshopper species on several types of vegetation and its relatively high flight capability.
BackgroundSupernumerary B chromosomes occur in addition to standard karyotype and have been described in about 15% of eukaryotes, being the repetitive DNAs the major component of these chromosomes, including in some cases the presence of multigene families. To advance in the understanding of chromosomal organization of multigene families and B chromosome structure and evolution, the distribution of rRNA and H3 histone genes were analyzed in the standard karyotype and B chromosome of three populations of the grasshopper Rhammatocerus brasiliensis.ResultsThe location of major rDNA was coincident with the previous analysis for this species. On the other hand, the 5S rDNA mapped in almost all chromosomes of the standard complement (except in the pair 11) and in the B chromosome, showing a distinct result from other populations previously analyzed. Besides the spreading of 5S rDNA in the genome of R. brasiliensis it was also observed multiple sites for H3 histone genes, being located in the same chromosomal regions of 5S rDNAs, including the presence of the H3 gene in the B chromosome.ConclusionsDue to the intense spreading of 5S rRNA and H3 histone genes in the genome of R. brasiliensis, their chromosomal distribution was not informative in the clarification of the origin of B elements. Our results indicate a linked organization for the 5S rRNA and H3 histone multigene families investigated in R. brasiliensis, reinforcing previous data concerning the association of both genes in some insect groups. The present findings contribute to understanding the organization/evolution of multigene families in the insect genomes.
Several techniques including C-banding, fluorochromes and silver staining were used to obtain information about heterochromatin patterns in the grasshopper B. coccineipes. Conventional staining showed a karyotype with 2n = 23 chromosomes in males and 2n = 24 in females, as well as XO:XX sex determination and acrotelocentric chromosomes. The medium-sized X chromosome was heteropycnotic positive at the beginning of prophase I and negative in metaphase I. C-banding revealed heterochromatic blocks in the pericentromeric regions of all chromosomes. Silver nitrate staining in this species showed three small bivalents (S9-S11) as nucleolar organizers with NORs located in the pericentromeric regions. CMA3-positive blocks were seen in pericentromeric regions of pairs M6, S9, S10 and S11. Sequential staining with CMA3/AgNO3 revealed homology between the CMA3-positive bands and NORs of the bivalents S9, S10 and S11. The CMA3-positive block of the bivalent M6 could represent a latent secondary NOR. The results obtained permit us to distinguish two categories of the constitutive heterochromatin in B. coccineipes. Algumas técnicas incluindo bandeamento C, fluorocromos e coloração com nitrato de prata foram utilizadas visando obter informações sobre os padrões de heterocromatina no gafanhoto B. coccineipes. A análise convencional mostrou um cariótipo com 2n = 23 cromossomos nos machos e 2n = 24 nas fêmeas, sistema XO de determinação do sexo e cromossomos acro-telocêntricos. O cromossomo X de tamanho médio mostrou-se heteropicnótico positivo no início da prófase I e negativo em metáfase I. O bandeamento C revelou blocos positivos nas regiões pericentroméricas de todos os cromossomos. A coloração com nitrato de prata nesta espécie evidenciou 3 bivalentes pequenos (S9-S11) portadores de nucléolos com as NORs localizadas nas regiões pericentroméricas. Blocos CMA3-positivos foram visualizados nas regiões pericentroméricas dos pares M6, S9, S10 e S11. Pela coloração seqüencial CMA3/AgNO3 observamos homologia entre as bandas CMA3-positivas e as NORs dos bivalentes S9, S10 e S11. A marcação CMA3-positiva do bivalente M6 poderia representar uma NOR secundária latente. Os resultados obtidos permitiram distinguir duas categorias de heterocromatina constitutiva em B. coccineipes
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