Karyotypic differentiation through chromosome fusion and number reduction in Imparfinis hollandi (Ostariophysi, Heptapteridae)

Margarido, Vladimir Pavan; Moreira‐Filho, Orlando

doi:10.1590/s1415-47572008000200012

Cited by 50 publications

(29 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…in Imparfinis hollandi, Margarido & Moreira-Filho, 2008). The hybridizations with telomeric sequences marked terminal regions of all chromosomes, additionally detecting an interstitial site on the short arm of the metacentric pair 1 near the centromere ( Fig.…”

Section: Discussionmentioning

confidence: 93%

“…It is notable that only if the latter explanation occurs it would be possible to maintain the structure of the telomeric sequence in an interstitial region (ITS). These rearrangements are among the most important events in karyotype evolution of mammals (Holmquist & Dancis, 1979), and some interesting examples are found in the evolution of fish groups (Giuliano-Caetano, 1998;Margarido & Moreira-Filho, 2008). In Auchenipteridae, only Ageneiosus has species with diploid number different from 58 (i.e., 54 or 56), thus it is possible that Robertsonian rearrangements may be involved with the chromosomal evolution of the group.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The role of chromosomal fusion in the karyotypic evolution of the genus Ageneiosus (Siluriformes: Auchenipteridae)

et al. 2013

Self Cite

View full text Add to dashboard Cite

Ageneiosus is the most widely distributed genus of the family Auchenipteridae among South American river basins. Although chromosome studies in the family are scarce, this genus has the largest number of analyzed species, with 2n = 54 to 56 chromosomes, differing from the rest of the family (2n = 58). This study aimed to analyze Ageneiosus inermis from the Araguaia River basin. The diploid number found was of 56 chromosomes. Heterochromatin was allocated in terminal region of most chromosomes, plus a pericentromeric heterochromatic block in pair 1, a pair distinguished by size in relation to other chromosomes pairs. AgNORs were detected in only one submetacentric chromosome pair, which was confirmed by FISH. 5S rDNA was present in only one metacentric chromosome pair. Hybridization with [TTAGGG] n sequence marked the telomeres of all chromosomes, in addition to an ITS in the proximal region of the short arm of pair 1. The repetitive [GATA] n sequence was dispersed, with preferential location in terminal region of the chromosomes. Ageneiosus has a genomic organization somewhat different when compared to other Auchenipteridae species. Evidences indicate that a chromosomal fusion originated the first metacentric chromosome pair in A. inermis, rearrangement which may be a basal event for the genus.Ageneiosus é o gênero da família Auchenipteridae mais amplamente distribuído em bacias da América do Sul. Apesar dos estudos cromossômicos nesta família serem escassos, este gênero tem o maior número de espécies analisadas, com número diploide variando de 54 a 56 cromossomos, o que difere do restante da família (2n = 58). Este estudo objetivou analisar Ageneiosus inermis da bacia do rio Araguaia. O número diploide encontrado foi de 56 cromossomos. A heterocromatina se mostrou localizada na região terminal da maioria dos cromossomos, além de um bloco heterocromático pericentromérico no par 1, um par facilmente distinguível no cariótipo pelo seu maior tamanho quando comparado aos outros pares do complemento. AgRONs foram detectadas em somente um par de cromossomos submetacêntricos, que foi confirmado pela FISH. 5S rDNA se mostrou presente em somente um par de cromossomos metacêntricos. A hibridização com a sequência [TTAGGG] n marcou os telômeros de todos os cromossomos, além de um ITS (sequência telomérica intersticial) na região proximal do braço curto do par 1. A sequência repetitiva [GATA] n se mostrou dispersa, com localização preferencial na região terminal dos cromossomos. Ageneiosus apresenta uma organização genômica um pouco diferente quando comparada a outras espécies de Auchenipteridae. As evidências indicam que uma fusão cromossômica originou o primeiro par de cromossomos metacêntricos de A. inermis, rearranjo que parece ser um evento basal para o gênero.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

The role of chromosomal fusion in the karyotypic evolution of the genus Ageneiosus (Siluriformes: Auchenipteridae)

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…cf. I. piperatus from the Juquiá River (VISSOTTO et al 2001, FENOCCHIO et al 2003, I borodini (MARGARIDO & MOREIRA-FILHO 2008) and I. hollandi (VISSOTTO et al 1999). This hypothesis is corroborated by the repositioning of the nucleolar organizing region in these species from chromosome pair 1 to other pairs in these species.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetics of Imparfinis schubarti (Siluriformes: Heptapteridae) from the Piumhi drainage, a diverted river in Minas Gerais State, Brazil

Kantek¹,

Peres²,

Buckup³

et al. 2009

Zoologia (Curitiba, Impr.)

Self Cite

View full text Add to dashboard Cite

Specimens of Imparfinis schubarti (Gomes, 1956) collected in the Piumhi river drainage, state of Minas Gerais, Brazil, were studied cytogenetically. The river was diverted from the Rio Grande Basin into the São Francisco basin in the early 1960s. All individuals presented 2n = 58 chromosomes, including 18 metacentric, 34 submetacentric and six subtelocentric chromosomes. A secondary constriction was observed in the interstitial region of the long arm of the largest chromosome pair, coinciding with the NOR. A single conspicuous heterochromatic block located in the largest pair of metacentric chromosomes was observed, adjacent to the secondary constriction. A detectable 18S rDNA probe hybridization region occurs in only one chromosome pair and is synthenic with the marking obtained with 5S rDNA probe. These results fit the cytogenetic pattern previously described for the genus Imparfinis Eigenmann & Norris, 1900

show abstract

“…Chromosome types were classified as metacentric (m), submetacentric (sm), subtelocentric (st) or acrocentric (a) based on the arm relationship criteria proposed by Levan et al (1964). Physical mapping of the 5S and 18S rDNA genes in the chromosomes was performed using fluorescence in situ hybridization (FISH) (Pinkel et al 1986, with modifications; Margarido and Moreira-Filho 2008), with probes obtained from the fish species Prochilodus argenteus (Hatanaka and Galetti 2004) and Leporinus elongatus (Martins and Galetti 1999), respectively. The probes were marked through nick translation, with digoxigenin-11-dUTP (5S rDNA) and biotin-16-dUTP (18S rDNA) (Roche, Basel, BS, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Karyotypic diversity and chromosome evolution in the armored catfishes Callichthyinae (Siluriformes, Callichthyidae)

et al. 2014

View full text Add to dashboard Cite

The Callichthyinae subfamily is composed of five genera with a small number of described species. Molecular cytogenetics studies show the scarcity of records for this subfamily. The aim of the present study was to employ cytogenetic parameters in the analysis of three species belonging to the subfamily Callichthyinae sampled from the Paraná River (Brazil). Callichthys callichthys had 2n = 56 chromosomes (26 m-sm + 30 st-a); Lepthoplosternum pectorale had 2n = 64 chromosomes (8 m-sm + 56 st-a); and Hoplosternum littorale had 2n = 60 chromosomes (8 m-sm + 52 st-a). Regarding the location of the nucleolar organizer regions (NORs) (Ag-staining and 18S rDNA-FISH), different situations were found: single NORs in H. littorale and C. callichthys; multiple NORs in L. pectorale, with intra-individual and inter-individual variation. Heterochromatin was observed in the centromeric region in the chromosomes of the three species. Equilocal, interstitial bands were also found in H. littorale. Physical mapping of 5S ribosomal genes by FISH shows eight 5S rDNA sites in C. callichthys; four 5S rDNA sites in H. littorale and six 5S rDNA sites in L. pectorale. Using cytogenetic markers (diploid number, chromosome formula, NORs, heterochromatin distribution pattern and 5S and 18S rDNA sites), the chromosomal evolution in Callichthyinae is presented: Callichthys shows mostly basal chromosomal conditions, with mostly derived chromosomal conditions verified for the Hoplosternum-Dianema clade and Megalechis-Lepthoplosternum clade.

show abstract

Karyotypic differentiation through chromosome fusion and number reduction in Imparfinis hollandi (Ostariophysi, Heptapteridae)

Cited by 50 publications

References 21 publications

The role of chromosomal fusion in the karyotypic evolution of the genus Ageneiosus (Siluriformes: Auchenipteridae)

The role of chromosomal fusion in the karyotypic evolution of the genus Ageneiosus (Siluriformes: Auchenipteridae)

Cytogenetics of Imparfinis schubarti (Siluriformes: Heptapteridae) from the Piumhi drainage, a diverted river in Minas Gerais State, Brazil

Karyotypic diversity and chromosome evolution in the armored catfishes Callichthyinae (Siluriformes, Callichthyidae)

Contact Info

Product

Resources

About