TaqI B1/B2 and -629A/C cholesteryl ester transfer protein (CETP) gene polymorphisms and their association with CETP activity and high-density lipoprotein cholesterol levels in a Tehranian population. Part of the Tehran Lipid and Glucose Study (TLGS)

Abstract

We examined the cholesteryl ester transfer protein (CETP) gene TaqI intron 1 B1/B2 polymorphism and the -629A/C CETP promoter polymorphism in respect to high-density lipoprotein cholesterol (HDL-C) in a healthy Iranian population taken from the Tehran Lipid and Glucose Study (TLGS). The relationship between CETP activity and HDL-C level was also determined along with body mass index, blood pressure and tobacco smoking status. PCR-RFLP used to amplify a segment of the CETP intron 1 TaqI (B2/B1) polymorphism from 1021 individuals and we selected 345 individuals from the lowest, middle and highest HDL-C deciles and investigated the -629A/C polymorphism. We also evaluated the CETP activity of 103 of these individuals, each with at least one homozygous allele. The presence of the TaqI B2 and -629A/C A alleles were significantly associated with increased HDL-C levels (B2B2 = 1.19 ± 0.31 mmolL -1 vs. B1B1 = 1.01 ± 0.2 mmol L -1 for p < 0.001; AA = 1.15 ± 0.41 mmol L -1 vs. CC = 0.95 ± 0.28 mmol L -1 for p < 0.001) and decreased the CETP activity (B1B1 = 67.8 ± 8.9 pmol L -1 vs. B2B2 = 62.6 ± 9.6 pmol L -1 for p < 0.01; CC = 68.6 ± 8.4 pmol L -1 vs. AA = 62.7 ± 9.7 pmol L -1 for p < 0.002). The frequencies were 0.382 for the TaqI B2 allele and 0.462 for the -629A/C A allele, with linkage disequilibrium analysis giving D = 0.0965 and D' = 0.4695. We demonstrated that the TaqI B1 and B2 alleles and the -629A/C A and C alleles were in linkage disequilibrium in our population and that there was a significant association between the B2 and A alleles and high HDL-C levels and low CETP activity. Linkage disequilibrium between the TaqI A and B2 alleles also detected.

Association between CETP Taq1B and LIPC -514C/T polymorphisms with the serum lipid levels in a group of Tehran's population: a cross sectional study

“…In comparison to B1B1 genotype, B2 hetero and homozygote subjects had respectively 2.26% and 7.36% higher levels of HDL-C. This positive role is in agreement with the results of the two other studies carried in Iran [43,45]. Thompson et al in their meta- analysis study, that covers the results of 92 studies in different ethnic groups with more than 113,000 subjects, have also found a 4.5% increase in HDL-C levels and a 0.9% and 2% decrease in LDL-C and TG levels respectively for each inherited B2 allele[44].…”

“…The frequency of the CETP "B2" allele in this study was 46.1% which is higher than the 38.21% previously found by Daneshpour et al[43] in a group of Tehran's residents. However, it is in accordance with the prevalence of 42% that was reported in White and East Asian populations[44].…”

Screening for Intestinal Parasites in Elective Surgery Patients in Endemic Areas: How Relevant Is It?

“…17 These observations are significantly important because low plasma HDL-C levels are associated with an increased risk of cardiovascular disease. Clinical evidence documents that an increase of 1% in the plasma HDL-C levels is associated with a reduction of 2% to 3% in cardiovascular morbidity and mortality.…”

Association of Apo E gene polymorphism with HDL level in Tehranian population

“…This could be attributed to industrialization of the country, modification of life style, unhealthy diet, decreased physical activity, and increased prevalence of hypertriacylglycerolmia, obesity and smoking [16]. Association of some genetic factors with low HDL-C levels has been studied in Iranians [30,31]. These results showed that there is a relation between low HDL-C level and some genetic factors.…”