Association of Apo E gene polymorphism with HDL level in Tehranian population

Abstract

The prevalence of cardiovascular risk factors, especially low level of high density lipoprotein cholesterol (HDL-C), is very high in Iran. The associations of apolipoprotein E (Apo E) polymorphism with lipid profile, especially high density lipoprotein (HDL) subfractions, were examined in Iranian population. A cross-sectional study of 1030 subjects (452 men and 578 women) from the Tehran Lipid and Glucose Study (TLGS) was performed. Serum triacylglycerol, cholesterol, FBS, HDL-C levels and its subfractions, Apo B and Apo A1 were determined and body mass index and blood pressure were measured. A segment of the mentioned gene was amplified by PCR and the polymorphisms were revealed by RFLP using HhaI restriction enzyme. Allele frequencies obtained for APOE Ã 2, APOE Ã 3, and APOE Ã 4 were 5.77, 85.92, and 8.3%, respectively. The presence of the e2 allele was significantly associated with increased serum HDL-C levels and its subfractions both in men and women except HDL3 in men. The LDL/HDL ratio was significantly lower in female. The relations were significant even after adjustment for age, sex, and BMI but hypertension, smoking, and diabetes status decreased the effect of Apo E2 on HDL-C and HDL subfractions. The observed genotype and allele frequencies were similar to those reported for other Caucasians samples. Apo E2 increased the level of HDL-C, HDL subfractions, and decreased the LDL/HDL ratio. These findings highlight the important effect of variation in this gene on lipid levels.

Assessment of SNP Interactions Affecting Total Cholesterol Over Time Using Logic Mixed Model: TLGS Study

“…Although association of main effects of these variables with total cholesterol have previously been shown in several studies (20,23,24), investigation for interaction effects among them on longitudinal total cholesterol level using logic mixed model approach was done for first time in this study.…”

ApoB (XbaI) polymorphism and lipid variation in Teharnian population

“…The detail of the biochemical analysis procedure presented in ref. 23. Serum glucose, total cholesterol, HDL-C, and triglyceride levels were measured as described previously [24].…”

Association ofCD36Gene Variants and Metabolic Syndrome in Iranians

“…The protocols for collection of clinical data have been described previously (Azizi et al, 2003;Daneshpour et al, 2010). Fasting serum glucose, triglycerides (TG), total cholesterol, and HDL-cholesterol (HDL-C) were measured using the enzymatic colorimetric method (Pars Azmon) by a Selectra 2 auto-analyzer (Vital Scientific).…”

“…For the present study, cases and controls were randomly selected from the TLGS participants with and without MetS (based on participant information and MetS definition), respectively. A detailed description of the methodology, rationale, and design of this study has been published elsewhere in detail (Azizi et al, 2002).This study was approved by the Medical Ethics Committee of the Research Institute for Endocrine Sciences at Shahid Beheshti University of Medical Science and informed consent was given by each participant.The protocols for collection of clinical data have been described previously (Azizi et al, 2003;Daneshpour et al, 2010). Fasting serum glucose, triglycerides (TG), total cholesterol, and HDL-cholesterol (HDL-C) were measured using the enzymatic colorimetric method (Pars Azmon) by a Selectra 2 auto-analyzer (Vital Scientific).…”