Polymorphism of exon 2-3 of bovine major histocompatibility complex class I BoLa-A gene

Abstract

The exon 2-3 region of bovine major histocompatibility complex (MHC) class I BoLa-A gene was investigated for polymorphisms in three breeds of cattle originated in the Indian subcontinent namely Sahiwal, Tharparkar, Hariana, as well as crossbred (Bos taurus x Bos indicus) cattle and Jersey, the exotic breed (Bos taurus). The PCR amplified fragment of 714 bp showed distinct DdeI-, TaqI-and HinfI-RFLP patterns, thus confirming a higher degree of polymorphism in this region. To our knowledge this is the first report of HinfI restriction patterns for BoLa-A exon 2-3. The sequencing results revealed a number of nucleotide substitutions in this region, which resulted in amino acid changes. The present investigation confirmed that MHC class I BoLa-A exon 2-3 is highly polymorphic in cattle.

RNA Sequencing (RNA-Seq) Based Transcriptome Analysis in Immune Response of Holstein Cattle to Killed Vaccine against Bovine Viral Diarrhea Virus Type I

“…Among identified immune-related genes, the bovine lymphocyte antigen (BoLA) caught the attention of the researcher, as it was directly involved in antigen presentation. The BoLA gene located on chromosomes BTA 23 [30], and generally known as the MHC of cattle, was reported to play an integral role in immune responsiveness and susceptibility to the diseases of the host animal [31]. MHC is a cell surface glycoprotein molecule, having the binding ability to foreign peptides, such as viral proteins, and provides context for the recognition of T-lymphocytes responsible for cell-mediated immunity [32,33].…”