Evaluation of polymorphism in ten microsatellite loci in Uruguayan sheep flocks

Abstract

The allele frequencies of 10 microsatellite loci previously described for sheep as BM1314, BM6526, OarFCB128, OarHH64, OarCP20, OarHH47, OarFCB48, OarHH35, OarHH72 and BM2508 were estimated for the Uruguayan flocks. A representative sample of 101 individuals composed by the two predominant breeds (76% Corriedale and 24% Australian Merino) was used. The sample did not show a significant tendency towards substructuring, in spite of presenting some significantly different allele frequencies between races. The Corriedale sample presents three loci in which the presence of null alleles is possible. The markers were highly variable, showing between 7 and 15 alleles each. The Polymorphism Information Content Index ranged from 0.63 to 0.87 and the Exclusion Probability from 0.39 to 0.75 for a cumulative Exclusion Probability of 99.98%. These results suggest the effectiveness of this set of loci for testing genetic relatedness. This is the first report of microsatellite variation in Corriedale.

Genetic characterization of the autochthonous sheep populations from Chiapas, Mexico

“…For breeds under conservation plans, the observed mean heterozygosity could be as low as 0.52 (Gutiérrez-Espeleta et al, 2000). The expected heterozygosities were similar to the Corriedale and Merino sheep from Uruguay (Tomasco et al, 2002).…”

“…Microsatellite markers have been shown to be useful tools for the analysis of genetic differentiation among sheep populations as well as aid in conservation decisions for genetic resources (Gutiérrez-Espeleta et al, 2000;Saitbekova et al, 2001;Tomasco et al, 2002;Rendo et al, 2004;Peter et al, 2005;Paiva et al, 2005), Here we describe microsatellite marker analyses that were carried out to help to clarify the genetic structure of the native sheep populations from Chiapas and its relationships with some Spanish breeds.…”

Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil

“…The microsatellite panel sanctioned by MAPA in 2004 for sheep and goat parentage verification in Brazil was based on available literature at the time (Luikart et al , 1999; Arranz et al , 2001; Crawford et al , 2000; Farid et al , 2000; Stahlberger-Saitbekova et al , 2001; Tomasco et al , 2002; Rychlik et al , 2003) and did not consider updates developed by the International Society of Animal Genetics and the genetic diversity of Brazilian breeds. Souza et al (2012) evaluated this panel in Santa Inês sheep and found that some of the used markers were not very informative due to the low number of observed alleles, PIC, and consequently, the low individual and combined probability of exclusion of the markers in the panel.…”

Genetic diversity and assessment of 23 microsatellite markers for parentage testing of Santa Inês hair sheep in Brazil

“…No ISAG-recommended loci were yet available. These eight markers were chosen based on available information from the literature on microsatellites used for genotyping related species such as goats (Luikart et al, 1999), the relatively few reports for wool sheep breeds (Diez-Tascon et al, 2000;Farid et al, 2000;Arranz et al, 2001;Stahlberger-Saitbekova et al, 2001;Tomasco et al, 2002;Rychlik et al, 2003) and scant genotype data for some Brazilian animals. Currently, however, the majority of Brazilian animals belong to hair breeds, meaning that this set of markers may not be the best choice as far as information content for identity and parentage testing.…”