Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis

Abstract

A fragment of 466 base pairs from a highly variable peripheral region of the mitochondrial D-loop of horses was amplified and analyzed by single stranded conformational polymorphism (SSCP). Fourteen distinct SSCP variants were detected in 100 horses belonging to four breeds (Arabian, ARB; Thoroughbred, TB; Argentinian Creole, ARC; and Peruvian Paso from Argentina, PPA). Each breed showed four to eight SSCP variants, many of which were shared between two or three of the studied breeds. Arabian horses were the most variable (eigth variants), with three variants unique to the breed. PPA and ARC showed two and one characteristic SSCP variants, respectively, while TB shared all its variants with at least one of the other breeds. An analysis based on the presence/absence of the variants revealed a closer relationship between PPA and TB, which was not completely unexpected considering the mixed ancestry of the PPA mares. The results also confirm the efficiency of SSCP to detect variability in horse mitochondrial DNA.

Mitochondrial D-loop sequence variation among Hucul horse

“…Figure 2 compares the present results with 13 sequences, as taken from GenBank database, of the Hucul horse. It is a well-known fact (Bowling et al, 2002;Hill et al, 2002;Kavar et al, 2002;Mirol et al, 2002;Cozzi et al, 2004;McGahern et al, Priskin et al, 2010) that the mtDNA D-loop region is very polymorphic. This also holds for the Huculs, as their genetic lines are not isolated (see Cieslak et al, 2010 for the most recent discussion of this topic).…”

“…The D-loop hypervariable region of the mtDNA is of particular interest because, unlike the protein-coding gene regions, it has a high level of sequence variation (Aquadro and Greenberg, 1983). Thus, the D-loop sequence variation combined with the lack of recombinations produce a highly informative tool for matrilineal relationship studies in horses (Bowling et al, 2000;Hill et al, 2002;Kavar et al, 2002;Mirol et al, 2002;Cozzi et al, 2004;Priskin et al, 2010). Here the D-loop hypervariable region of the mtDNA is investigated in as many as 15 maternal lines of the Hucul breed, for the total of 165 horses.…”

Analisis Genetika Molekuler Kuda Sumba Berdasarkan Urutan D-Loop Mitokondria

“…Cozzi et al (2004) telah berhasil membuat pohon filogenetika di antara galur kuda di Italia berdasarkan urutan daerah bukan penyandi protein (D-loop). Mirol et al (2002) melaporkan keberhasilan menganalisis keragaman genetika dan hubungan kekerabatan di antara empat galur kuda di Argentina (kuda Argentina Creole, kuda Peruvian Paso, kuda Arabian, dan kuda Thoroughbred) berdasarkan sequence D-loop. Cothran (2005) juga melaporkan keberhasilan dalam melakukan analisis genetika beberapa kuda di Lithuania berdasarkan sequence control region D-loop mitochondria dengan primer daerah bukan pengode protein (D-loop) ECLOOPF 5'-CGCACATTACCCTGGTCTTG-3' dan ECLOOPR 5'-GAACCAGATGCCAGGTATG-3'.…”

Sequence and analysis of the mitochondrial DNA control region in the sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

“…The molecular characterization of this insect is important for the development of different analyses such as the phylogenetic studies. Concerning the assessment of intra and interspecific variations, the analysis of the mitochondrial (mt) noncoding segment called Control Region (CR) has proven to be a powerful tool due to the high variability than other mitochondrial genome regions (Harrison, 1989;Mirol et al, 2002). The mitochondrial genome of several insect species has been sequenced (http://amiga.cbmeg.unicamp.br).…”