Mitochondrial D-loop sequence variation among Hucul horse

Czerneková, V.; Kott, T.; Majzlík, I.

doi:10.17221/6992-cjas

“…MtDNA D-loop sequence variation was used to discover the genetic relationships among different horse breeds and population around the world. Czerneková et al [7] analyzed 165 Hucul horses mtDNA and the result revealed 38 polymorphic sites resulting in 14 haplotypes belonging to six haplogroups. This genetic information is the most important information used for breed conservations.…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial DNA genetic variations among four horse populations in Egypt

Othman

¹

,

Mahrous

²

,

Shafey

³

2017

Journal of Genetic Engineering and Biotechnology

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Horses are one of the early domesticated animals in the world that changed societies and civilizations on a continent-wide scale. Due to the rare information about the genetic characterization of different horse populations in Egypt, this study aimed to identify the genetic biodiversity and relationships between four horse populations reared in Egypt. Genomic DNA was extracted and mtDNA region was amplified using polymerase chain reaction (PCR). The alignment of 384-bp amplified fragments showed the presence of 41 polymorphic sites resulting in 29 haplotypes which their sequences were submitted to GenBank under the accession numbers: KX909898-KX909926.The phylogeny tree for tested horses declared the presence of mixing maternal lineages between the four tested populations but still there are some separated lineages especially for Arabian and Thoroughbred horses. The sequences of 72 tested sequences were aligned with 13 published sequences as references, 11 of them for different Equus caballus whereas the other two reference sequences for Equus burchellii and Equus asinus. The results showed that all tested horses from the four populations are grouped with reference sequences of Equus caballus and separated from the other two reference sequences of Equus burchellii and Equus asinus.It is concluded that sequence analysis of mtDNA control region is still the most informative tool for the identification of genetic biodiversity and phylogeny of different horse breeds and populations. The horse populations reared in Egypt possess low genetic diversity and all of them are belonged to Equus caballus breed.

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“…A determination of phylogenetic relationships and the verification of stud book accuracy could be the first steps in the maintenance of the genetic pool ( Bodó, Alderson & Langlois, 2005 ). To date, contrary to the situation with the Hucul, which is another, but well-studied native Hungarian horse breed ( Czerneková, Kott & Majzlík, 2013 ; Georgescu et al, 2011 ; Kusza et al, 2013 ), no data are available on the genetic structure and diversity of the Gidran. In this regard, through DNA sequence comparisons of CYTB and the D-loop, we investigated matrilineal diversity for Gidran horses in Hungary.…”

Section: Discussionmentioning

confidence: 96%

Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers

Sziszkosz

¹

,

Mihok

²

,

Jávor

³

et al. 2016

PeerJ

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The Gidran is a native Hungarian horse breed that has approached extinction several times. Phylogenetic analysis of two mitochondrial markers (D-loop and cytochrome-b) was performed to determine the genetic characterization of the Gidran for the first time as well as to detect errors in the management of the Gidran stud book. Sequencing of 686 bp of CYTB and 202 bp of the D-loop in 260 mares revealed 24 and 32 haplotypes, respectively, among 31 mare families. BLAST analysis revealed six novel CYTB and four D-loop haplotypes that have not been previously reported. The Gidran mares showed high haplotype (CYTB: 0.8735 ± 0.011; D-loop: 0.9136 ± 0.008) and moderate nucleotide (CYTB: 0.00472 ± 0.00017; D-loop: 0.02091 ± 0.00068) diversity. Of the 31 Gidran mare families, only 15 CYTB (48.4%) and 17 D-loop (54.8%) distinct haplotypes were formed using the two markers separately. Merged markers created 24 (77.4%) mare families, which were in agreement with the mare families in the stud book. Our key finding was that the Gidran breed still possesses high genetic diversity despite its history. The obtained haplotypes are mostly consistent with known mare families, particularly when the two mtDNA markers were merged. Our results could facilitate conservation efforts for preserving the genetic diversity of the Gidran.

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“…A determination of phylogenetic relationships and the verification of stud book accuracy could be the first steps in the maintenance of the genetic pool (Bodó et al 2005). To date, contrary to the situation with the Hucul, which is another, but well-studied native Hungarian horse breed (Czerneková et al 2013;Georgescu et al 2011;Kusza et al 2013) (Cothran et al 2005;Kusza et al 2013).…”

Section: Resultsmentioning

confidence: 92%

Peer Review #1 of "Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers (v0.2)"

2016

0

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The Gidran is a native Hungarian horse breed that has approached extinction several times. Phylogenetic analysis of two mitochondrial markers (D-loop and cytochrome-b) was performed to determine the genetic characterization of the Gidran for the first time as well as to detect errors in the management of the Gidran stud book. Sequencing of 686 bp of CYTB and 202 bp of the D-loop in 260 mares revealed 24 and 32 haplotypes, respectively, among 31 mare families. BLAST analysis revealed six novel CYTB and four D-loop haplotypes that have not been previously reported. The Gidran mares showed high haplotype (CYTB: 0.8735 ± 0.011; D-loop: 0.9136 ± 0.008) and moderate nucleotide (CYTB: 0.00472 ± 0.00017; D-loop: 0.02091 ± 0.00068) diversity. Of the 31 Gidran mare families, only 15 CYTB (48.4%) and 17 D-loop (54.8%) distinct haplotypes were formed using the two markers separately. Merged markers created 24 (77.4%) mare families, which were in agreement with the mare families in the stud book. Our key finding was that the Gidran breed still possesses high genetic diversity despite its history. The obtained haplotypes are mostly consistent with known mare families, particularly when the two mtDNA markers were merged. Our results could facilitate conservation efforts for preserving the genetic diversity of the Gidran.

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Mitochondrial D-loop sequence variation among Hucul horse

Cited by 8 publications

References 18 publications

Mitochondrial DNA genetic variations among four horse populations in Egypt

Mitochondrial DNA genetic variations among four horse populations in Egypt

Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers

Peer Review #1 of "Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers (v0.2)"

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