Effect of gamma-radiation and sodium azide on quantitative characters in rice (Oryza sativa L.)

Abstract

Seeds of rice cultivar IAC-1246 received single and combined treatments of 10 or 20 Krad gamma-rays and 0.5 mM sodium azide (SA). The experiments were carried out to assess the effect of treatments on the mean and variance in second generation plants of the following quantitative traits: number of days to flowering (NDF), culm length (CL) and tiller number (TN). In general, the mutagenic treatments increased variance, but did not change the mean for the characters NDF and CL in the M2 generation. There was no increase in the mean or variance of TN. The combined treatments of gamma-rays and SA resulted in larger variance in CL than for the treatments with gamma-rays alone, but not higher than obtained with SA alone. Combined treatments with gamma-rays and SA did not increase the variance of NDF and TN when compared with the corresponding single treatments.<br>Sementes do cultivar de arroz IAC-1246 receberam tratamento individual e combinado de 10 e 20 Krad de raios-gama e 0,5 de azida sódica (SA). O experimento foi realizado para avaliar o efeito dos tratamentos sobre a média e a variância de plantas M2 nos seguintes caracteres quantitativos: número de dias para a maturação (NDF), comprimento de colmo (CL) e número de perfílhos (TN). Em geral, os tratamentos mutagênicos incrementaram a variância, sem mudar a média para os caracteres NDF e CL na geração M2. Todavia, não foi encontrado nenhum incremento seja para a média ou para a variância no caráter TN. Para o caráter CL, os tratamentos combinados de raios-gama e SA mostraram maior variância que aquela observada no tratamento individual com raios-gama, não sendo superior que aquela correspondente ao tratamento individual com SA. Para os caracteres NDF e TN, os tratamentos combinados de raios-gama e SA não incrementaram a variância, quando comparados aos correspondentes tratamentos individuais

Effect of Sodium Azide on Quantitative and Qualitative Stem Traits in the M2 Generation of Ethiopian Sesame (Sesamum indicum L.) Genotypes

“…The short internode length and dwarfing trait in mutant dw607 is reported to be controlled by a recessive gene allele (Sidwf1) [ 42 ]. Previous studies [ 60 , 61 ] have reported the role of chemical mutagens in improving genetic diversity in higher plants. Mutants such as barley, cotton, rice, peanuts, wheat, and beans produced facilitate the identification, isolation, and cloning of genes used in designing crops with improved quality and yield traits [ 62 ].…”

Induction of maize chromosome altered plants by seeds irradiation pre-soaked in metronidazole

“…PATRA et al (1998) using the combination of EMS and gamma rays in Papaver somniferum also reported the efficiency in the combination of physical and chemical mutagenics in the production of mutants. Other effects of physical and chemical mutagenics were obtained by MONTALVAN and ANDO (1998), that reported the increase of variability for stem length and number of days for maturation in rice (Oriza sativa), after the treatment of seeds with gamma rays and AS.…”