Evaluation of the performance of the modified direct agglutination test (MAT) for detection of Toxoplasma gondii antibodies in dogs

Cañón-Franco, William Alberto; Bergamaschi, Denise Pimentel; Richtzenhain, Leonardo José; Nogueira, Yeda L.; Camargo, Luís Marcelo Aranha; Souza, S. L. P; Gennari, Solange Maria

doi:10.1590/s1413-95962003000600009

Cited by 6 publications

(2 citation statements)

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“…Although the sensitivity of the MAT was lower than that of ELISA and IFAT, its accuracy was higher and very similar to that of IHAT. In addition, the MAT is a simple, low-cost method that does not use a specific conjugate, whereas IFAT and ELISA require the use of a species-specific conjugate and the interpretation of the IFAT result is subjective (Ca ñón-Franco et al, 2003;Millar et al, 2012). Therefore, the MAT is the most indicated method among those tested for the diagnosis of T. gondii infection in chickens in epidemiological surveys designed to detect areas of environmental contamination.…”

Section: Discussionmentioning

confidence: 99%

Sensitivity and specificity of serological tests, histopathology and immunohistochemistry for detection of Toxoplasma gondii infection in domestic chickens

Alves

Boechat

Macedo-Couto

et al. 2014

Veterinary Parasitology

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Section: Discussionmentioning

confidence: 99%

Sensitivity and specificity of serological tests, histopathology and immunohistochemistry for detection of Toxoplasma gondii infection in domestic chickens

Alves

Boechat

Macedo-Couto

et al. 2014

Veterinary Parasitology

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“…The sensitivity and specificity of the IFAT changes based on the cutoff values established (Björkman & Uggla, 1999). However, some authors have used this test as the gold standard to evaluate the accuracy of other tests for infection by both parasites (Cañón Franco et al, 2003). Meireles et al used negative and positive T. gondii controls determined by the IFAT to standardize the enzyme-linked immunosorbent assay (ELISA) (Meireles et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

Seroprevalence and incidence of Toxoplasma gondii and Neospora caninum infection in naturally exposed domestic dogs from a rural area of São Paulo state, Brazil

Sevá

Chiebáo

Brandão

et al. 2020

Rev. Bras. Parasitol. Vet.

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Dogs are hosts of the protozoans Toxoplasma gondii, which causes an important public health disease, and Neospora caninum. Studies that have evaluated toxoplasmosis and neosporosis for prolonged periods in dog populations are rare. We analyzed infection by both parasites in a domestic dog population over three consecutive years in São Paulo state, Brazil. In the 1st, 2nd and 3rd years of collection, 181, 193 and 172 domiciles were visited, and blood samples of 331, 371 and 348 dogs were collected for antibody serology, respectively. The seroprevalence of T. gondii in each year was 27.2%, 22.5% and 43.9%, respectively, and that of N. caninum was 7.8%, 4.8% and 6.8%, respectively. The incidence rates for T. gondii in the 2nd and 3rd collections were 13.2% and 30.0%, and those for N. caninum were 3.3% and 4.4%, respectively. Positive and negative serological conversions for both agents occurred at high frequencies during the study period. This study reveals the canine population’s serological profile and demonstrates the constant exposure of dogs to the investigated pathogens, indicating the need for prevention and control measures in the region.

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Prevalência de anticorpos anti - Toxoplasma gondii em avestruzes (Struthio camelus) de criatórios comerciais no estado de São Paulo

Contente

Domingues

Silva

2009

Braz. J. Vet. Res. Anim. Sci.

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A toxoplasmose é uma zoonose cosmopolita causada pelo protozoário Toxoplasma gondii, podendo acometer mamíferos e aves. O presente estudo teve como objetivo estimar a prevalência do Toxoplasma gondii em avestruzes (Struthio camelus) de criatórios comerciais do estado de São Paulo, como forma de auxiliar no conhecimento do comportamento e importância do parasito nesta espécie animal. Foram colhidas 195 amostras de soro de avestruzes, provenientes de Sorocaba, Campinas, São Carlos, Araçatuba, São Paulo, Vale do Ribeira, Botucatu e São José do Rio Preto, estado de São Paulo. As amostras foram analisadas pela Técnica de Aglutinação Direta Modificada (MAT), para a pesquisa de anticorpos anti - Toxoplasma gondii. Os exames sorológicos revelaram 14,36% de animais sororreagentes ao T. gondii. A titulação mínima considerada foi a diluição maior ou igual a 1:16, e a maior diluição encontrada foi 1:16384. Não foi constatada diferença significativa entre os sexos. Apenas duas regiões (São Paulo e São José do Rio Preto) não apresentaram animais sororreagentes. Esses resultados salientam a importância de um estudo mais aprofundado sobre a infecção em avestruzes, e também sobre as práticas de manejo que venham a minimizar o risco de transmissão da toxoplasmose para essas aves e, por conseqüência, para o consumidor final.

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Evaluation of the performance of the modified direct agglutination test (MAT) for detection of Toxoplasma gondii antibodies in dogs

Cited by 6 publications

References 0 publications

Sensitivity and specificity of serological tests, histopathology and immunohistochemistry for detection of Toxoplasma gondii infection in domestic chickens

Sensitivity and specificity of serological tests, histopathology and immunohistochemistry for detection of Toxoplasma gondii infection in domestic chickens

Seroprevalence and incidence of Toxoplasma gondii and Neospora caninum infection in naturally exposed domestic dogs from a rural area of São Paulo state, Brazil

Prevalência de anticorpos anti - Toxoplasma gondii em avestruzes (Struthio camelus) de criatórios comerciais no estado de São Paulo

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