Padronização da lesão de medula espinal em ratos Wistar

Rodrigues, Nilson Rodnei; Letaif, Olavo Biraghi; Cristante, Alexandre Fogaça; Marcon, Raphael Martus; Oliveira, Reginaldo Perilo; Filho, Tarcísio Eloy Pessoa de Barros

doi:10.1590/s1413-78522010000400001

Cited by 19 publications

(18 citation statements)

References 17 publications

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“…[1][2][3][4][5][6] In our protocol we opted for the "MASCIS IMPACTOR" model, which uses weight drop from a standardized height causing spinal cord contusion in rats, after which they are assessed in their locomotor function. 1,2,7 In this experimental study we used Wistar rats due to the low cost and ease in their obtainment.…”

Section: Discussionmentioning

confidence: 99%

“…(Figures 11 and 12) A dose of 25mg/kg of cephalothin was administered subcutaneously. 4 The animals were kept in cages (60x40cm) in groups of 3 or 4, and provided with feed and water ad libitum. The massages for vesicle emptying were performed daily, in addition to the evaluation of possible complications, such as urinary infection.…”

Section: Spinal Cord Contusionmentioning

confidence: 99%

“…[1][2][3] This knowledge applied to clinical and surgical treatments is used to reduce morbidity and mortality in patients with spinal cord injury. 4 It is important to have a standardized assessment applied in experimental studies to determine the effects of each therapeutic intervention that one wishes to study. Some assessment criteria have been presented in literature, and the scale proposed by Basso, Beattie and Bresnahan, BBB, is that used in this study.…”

Section: Introductionmentioning

confidence: 99%

“…Some assessment criteria have been presented in literature, and the scale proposed by Basso, Beattie and Bresnahan, BBB, is that used in this study. 1,2,4 Defined as a scale of 21 points that observes recovery of locomotion in the hind limb after thoracic spinal cord injury in rats, this scale represents a detailed and organized categorization of the recovery of movements in the lower limb following the spinal cord lesion. Each index represents a single and sequential stage of behavioral recovery.…”

Section: Introductionmentioning

confidence: 99%

“…5 The objective of this study is to evaluate, using the BBB scale, the severity of lesions at different levels of height made using a standardized experimental spinal cord injury model known as a the "Mascis Impactor". 4 The study subjects were 20 young male adult Wistar rats, with mean age 20 of weeks, weighing around 350g, from the Centro de Bioterismo da Faculdade de Medicinada USP, divided into four groups of five animals. The group divisions were performed as follows: Control group: we performed only approach and laminectomy at the level of T9 Group 2: 12.5 mm representing mild injury Group 3: 15 mm representing moderate injury Group 4: 50 mm representing severe injury.…”

Section: Introductionmentioning

confidence: 99%

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Modelo experimental de lesão medular e protocolo de avaliação motora em ratos wistar

Santos¹,

Cristante²,

Marcon³

et al. 2011

Acta ortop. bras.

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Objectives: To determine a standardized spinal cord contusion model and a method for motor assessment in rats with paraplegia. Methods: This study used 20 Wistar rats divided into 4 groups according to level of severity of spinal cord injury; standardized intermediate lesions were made through system MASCIS IMPACTOR (Multicenter Animal Spinal Cord Injury Study): group 1, 12.5mm (mild injury); group 2,25mm (moderate injury); group 3,50mm (severe injury; in the group 4 the animals suffered no injury (control group). Motor function was assessed after 48 hours, using the scale proposed by Basso, Beattie and Bresnahan. Results: Using the model, we observed that the mild contusions (12.5mm height) were effective, and the animals presented acute urinary tract infection one week after the injury. Moderate contusions (25mm height) were effective, and the animals presented urinary infection until 2 weeks after injury. The severe contusions (50mm height) were effective, and the animals presented urinary infection for 3 to 4 weeks and autophagy. Conclusion: The model of spinal cord injury using the system MASCIS IMPACTOR and the functional assessment proposed by Basso, Beattie and Bresnahan is reproducible and can be used, enabling information exchange among different researchers.

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Section: Discussionmentioning

confidence: 99%

Section: Spinal Cord Contusionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Modelo experimental de lesão medular e protocolo de avaliação motora em ratos wistar

Santos¹,

Cristante²,

Marcon³

et al. 2011

Acta ortop. bras.

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“Low-intensity laser therapy effect on the recovery of traumatic spinal cord injury”

et al. 2014

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Scientific advances have been made to optimize the healing process in spinal cord injury. Studies have been developed to obtain effective treatments in controlling the secondary injury that occurs after spinal cord injury, which substantially changes the prognosis. Low-intensity laser therapy (LILT) has been applied in neuroscience due to its anti-inflammatory effects on biological tissue in the repairing process. Few studies have been made associating LILT to the spinal cord injury. The objective of this study was to investigate the effect of the LILT (GaAlAs laser-780 nm) on the locomotor functional recovery, histomorphometric, and histopathological changes of the spinal cord after moderate traumatic injury in rats (spinal cord injury at T9 and T10). Thirty-one adult Wistar rats were used, which were divided into seven groups: control without surgery (n = 3), control surgery (n = 3), laser 6 h after surgery (n = 5), laser 48 h after surgery (n = 5), medullar lesion (n = 5) without phototherapy, medullar lesion + laser 6 h after surgery (n = 5), and medullar lesion + laser 48 h after surgery (n = 5). The assessment of the motor function was performed using Basso, Beattie, and Bresnahan (BBB) scale and adapted Sciatic Functional Index (aSFI). The assessment of urinary dysfunction was clinically performed. After 21 days postoperative, the animals were euthanized for histological and histomorphometric analysis of the spinal cord. The results showed faster motor evolution in rats with spinal contusion treated with LILT, maintenance of the effectiveness of the urinary system, and preservation of nerve tissue in the lesion area, with a notorious inflammation control and increased number of nerve cells and connections. In conclusion, positive effects on spinal cord recovery after moderate traumatic spinal cord injury were shown after LILT.

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The locomotor system as seen in Brazilian medical journals: a restricted collection of papers

Patel¹,

Caramelli²,

Silva³

2011

Revista da Associação Médica Brasileira

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Padronização da lesão de medula espinal em ratos Wistar

Cited by 19 publications

References 17 publications

Modelo experimental de lesão medular e protocolo de avaliação motora em ratos wistar

Modelo experimental de lesão medular e protocolo de avaliação motora em ratos wistar

“Low-intensity laser therapy effect on the recovery of traumatic spinal cord injury”

The locomotor system as seen in Brazilian medical journals: a restricted collection of papers

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