Modelo experimental de lesão medular e protocolo de avaliação motora em ratos wistar

Abstract

Objectives: To determine a standardized spinal cord contusion model and a method for motor assessment in rats with paraplegia. Methods: This study used 20 Wistar rats divided into 4 groups according to level of severity of spinal cord injury; standardized intermediate lesions were made through system MASCIS IMPACTOR (Multicenter Animal Spinal Cord Injury Study): group 1, 12.5mm (mild injury); group 2,25mm (moderate injury); group 3,50mm (severe injury; in the group 4 the animals suffered no injury (control group). Motor function was assessed after 48 hours, using the scale proposed by Basso, Beattie and Bresnahan. Results: Using the model, we observed that the mild contusions (12.5mm height) were effective, and the animals presented acute urinary tract infection one week after the injury. Moderate contusions (25mm height) were effective, and the animals presented urinary infection until 2 weeks after injury. The severe contusions (50mm height) were effective, and the animals presented urinary infection for 3 to 4 weeks and autophagy. Conclusion: The model of spinal cord injury using the system MASCIS IMPACTOR and the functional assessment proposed by Basso, Beattie and Bresnahan is reproducible and can be used, enabling information exchange among different researchers.

A computerized system for the application of Basso, Beattie and Bresnahan scale in Wistar rats

“…In this study, the two heads of the laboratory of FMUSP, with 15 years of experience in the application of the scale and who published sereval papers in this line of research, were considered the gold standard in the evaluation. 15 , 16 The BBB scale involves difficulty in the assignment of scores. 14 A computer program was developed to help reduce the discrepancy of scores assigned to the same rat by different observers, in an attempt to bring the evaluation of a researcher with limited experience in the scale closer to that of researchers experienced in its application.…”

“…The two heads of the laboratory (AFC and GBS), with 15 years of experience in the application of the scale, initially evaluated the images of the 12 rats and, in mutual agreement, assigned a single score to each animal, based on the side of greater motor deficit or the side with the lowest score. The results of the two more experienced evaluators, with papers published on the subject of spinal cord injury, 15 , 16 were considered the gold standard in the evaluation and used as a reference.…”

Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury

“…For local anesthesia, we used lidocaine hydrochloride with epinephrine. Anesthesia was confirmed by the absence of corneal and tail reflexes and hind paw reactions at compression 34. The anesthetic agents took effect after 5 min and lasted for at least 2 h. For MEP evaluations, we administered pentobarbital (55-75 mg/kg) intraperitoneally and used the lethal dose of 140 mg/kg, followed by potassium chloride intravenously for euthanasia, on day 42.…”

Estudo dos efeitos do monossialogangliosídeo (GM1) administrado pela via transdérmica por laser a baixa temperatura, após lesão medular experimental em ratos

“…Há necessidade de novas pesquisas científicas rigorosas, pois não existem bases sólidas para a elucidação do complexo sistema de reações que ocorrem após a lesão espinal (Amador e Guest, 2005;Rossignol et al, 2007 (Rodrigues, 1999;Vialle et al, 1999;Young, 2002;Tebet et al, 2003;Marcon, 2006Marcon, , 2009Rodrigues et al, 2010;Galvão et al, 2011;Santos et al, 2011).…”